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The proteinuria od cadmium poisoning : analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isolation of a new low molecular weight component

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Abstract

A low molecular weight β2-globulin which goes not correspond to any known protein has been isolated from urine of a cadmium-exposed worker with proteinuria. The isolation procedure included chromatography on Sephadex G-75 and DEAE-cellulose, followed by preparative agarose gel electrophoresis.
The purified protein was homogeneous in ultracentrifugation and in electrophoresis in agarose gel. Electrophoresis in polyacrylamide gel containing sodium dodecyl sulphate confirmed the homogeneity of the preparation and suggested a molecular weight of about 21.000. No carbohydrate was detected by the anthrone reaction. Absorbance was maximal at 279 nm. This protein is apparently devoid of cysteine, methionine and histidne. Trace amounts of the protein have been found in normal human urine


Dissertation
Noninvasive measuring methods for the investigation of irritant patch test reactions : a study of patients with hand eczema, atopic dermatitis and controls
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Year: 1992 Publisher: Trondheim : Almqvist & Wiksell,

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Gel Electrophoresis of Nucleic Acids : A Practical Approach
Authors: ---
ISBN: 0199630828 0199630836 Year: 1990 Publisher: Oxford IRL press

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Now in a new second edition, this popular book includes a wealth of practical information on the much-used techniques of gel electrophoresis. Authoritative and completely current, the volume provides readers with an up-to-date, "how-to" guide to this growing field.

Keywords

Nucleic acids --- Gel electrophoresis --- Acides nucléiques --- Electrophorèse en gel --- Analysis --- Analyse --- DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- -577.113 --- 57.088.3 --- 543.545 --- Elektroforese --- Polynucleotides --- Biomolecules --- Electrophoresis --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Methods and techniques for separation,fractionation and purification. --- Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- Gel electrophoresis. --- Basic Sciences. Chemistry --- Analysis. --- Analytical Chemistry --- Analytical Chemistry. --- 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- 577.113 Nucleic acids --- Acides nucléiques --- Electrophorèse en gel --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- 577.113 --- Methods and techniques for separation,fractionation and purification

Gel electrophoresis of proteins : a practical approach
Authors: ---
ISBN: 0904147223 9780904147223 Year: 1985 Publisher: London IRL press

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Gel electrophoresis of proteins
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ISBN: 0191565636 1280375450 9786610375455 0585483515 9780191565632 0199636419 9780199636419 0199636400 9780199636402 6610375453 9780585483511 9781280375453 1383049270 Year: 1998 Volume: 197 Publisher: Oxford New York Oxford University Press

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This text presents the best methods, hints and tips on core procedures for running protein gels that will be widely applicable in the laboratory situation. The book's contributors are researchers writing primarily for researchers.

Keywords

Proteins --- Gel electrophoresis. --- Gel electrophoresis --- Electrophoresis --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Electrochemical Techniques --- Chemistry Techniques, Analytical --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Electrophoresis, Polyacrylamide Gel --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Gene Products, Protein --- Gene Proteins --- Protein --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Electrochemical Technics --- Electrochemical Technic --- Electrochemical Technique --- Technic, Electrochemical --- Technics, Electrochemical --- Technique, Electrochemical --- Techniques, Electrochemical --- Electrophoreses --- Analysis. --- Analysis --- Electrophoresis.


Book
Modern Grinding Technology and Systems
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ISBN: 3038429376 3038429384 Year: 2019 Publisher: MDPI - Multidisciplinary Digital Publishing Institute

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This specialist edition features key innovations in the science and engineering of new grinding processes, abrasives, tools, machines, and systems for a range of important industrial applications. Topics written by invited, internationally recognized authors review the advances and present results of research over a range of well-known grinding processes. A significant introductory review chapter explores innovations to achieve high productivity and very high precision in grinding. The reviewed applications range from grinding systems for very large lenses and reflectors, through to medium size grinding machine processes, and down to grinding very small components used in MEMS . Early research chapters explore the influence of grinding wheel topography on surface integrity and wheel wear. A novel chapter on abrasive processes also addresses the finishing of parts produced by additive manufacturing through mass finishing. Materials to be ground range from conventional engineering steels to aerospace materials, ceramics, and composites. The research findings highlight important new results for avoiding material sub-surface damage. The papers compiled in this book include references to many source publications which will be found invaluable for further research, such as new features introduced into control systems to improve process efficiency. The papers also reflect significant improvements and research findings relating to many aspects of grinding processes, including machines, materials, abrasives, wheel preparation, coolants, lubricants, and fluid delivery. Finally, a definitive chapter summarizes the optimal settings for high precision and the achievement of centerless grinding stability.


Book
Current Trends and Perspectives in the Application of Polymeric Materials for Wastewater Treatment
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Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Water is indispensable to the functioning of most known life forms, and good water quality is essential to human health, social and economic development, and ecosystem functioning. Nonetheless, population growth has been leading to the degradation and depletion of fresh water resources. Under these circumstances, ensuring sufficient and safe water supplies for everyone is one of the Sustainable Development Goals (SDGs) set by the United Nations General Assembly in 2015 for the year 2030. For this goal to be achieved, the development and implementation of appropriate and efficient wastewater treatments that allow us to reduce water pollution is a major challenge.In view of the relevant contribution that polymers and polymeric materials may have in the conservation of the aquatic environment, namely by their application in wastewater treatment, original research and review papers on “Current trends and perspectives in the application of polymeric materials for wastewater treatment” were here brought together. For sure, this set of papers will be helpful and inspiring for readers interested in this topic.


Book
Current Trends and Perspectives in the Application of Polymeric Materials for Wastewater Treatment
Authors: ---
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Water is indispensable to the functioning of most known life forms, and good water quality is essential to human health, social and economic development, and ecosystem functioning. Nonetheless, population growth has been leading to the degradation and depletion of fresh water resources. Under these circumstances, ensuring sufficient and safe water supplies for everyone is one of the Sustainable Development Goals (SDGs) set by the United Nations General Assembly in 2015 for the year 2030. For this goal to be achieved, the development and implementation of appropriate and efficient wastewater treatments that allow us to reduce water pollution is a major challenge.In view of the relevant contribution that polymers and polymeric materials may have in the conservation of the aquatic environment, namely by their application in wastewater treatment, original research and review papers on “Current trends and perspectives in the application of polymeric materials for wastewater treatment” were here brought together. For sure, this set of papers will be helpful and inspiring for readers interested in this topic.

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