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A low molecular weight β2-globulin which goes not correspond to any known protein has been isolated from urine of a cadmium-exposed worker with proteinuria. The isolation procedure included chromatography on Sephadex G-75 and DEAE-cellulose, followed by preparative agarose gel electrophoresis.
The purified protein was homogeneous in ultracentrifugation and in electrophoresis in agarose gel. Electrophoresis in polyacrylamide gel containing sodium dodecyl sulphate confirmed the homogeneity of the preparation and suggested a molecular weight of about 21.000. No carbohydrate was detected by the anthrone reaction. Absorbance was maximal at 279 nm. This protein is apparently devoid of cysteine, methionine and histidne. Trace amounts of the protein have been found in normal human urine
Cadmium --- Proteinuria --- Electrophoresis, Polyacrylamide Gel --- Sodium Dodecyl Sulfate --- Molecular Weight
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Patch tests --- Dermatitis, atopic --- Sodium dodecyl sulfate --- Irritants --- Hand --- Eczema --- Patch tests --- Dermatitis, atopic --- Sodium dodecyl sulfate --- Irritants --- Hand --- Eczema
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Analytical biochemistry --- Clinical chemistry --- Electrophoresis, Polyacrylamide gel --- Isoelectric focusing --- Congresses --- Polyacrylamide gel electrophoresis --- Electrophoresis, Polyacrylamide Gel. --- Isoelectric Focusing. --- -Polyacrylamide gel electrophoresis --- -Electrophoresis, Polyacrylamide gel --- PAGE (Biochemistry) --- Polyacrylamide disc electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Electrofocusing --- Focusing, Isoelectric --- Chemistry, Analytic --- Chemistry, Organic --- Electrophoresis --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Qualitative --- Eiwitten --- Elektroforese --- Congresses. --- Eiwitten. --- Elektroforese. --- -Congresses --- Electrophoresis, Polyacrylamide Gel --- Isoelectric Focusing --- Analytical chemistry --- Polyacrylamide gel electrophoresis - Congresses --- Isoelectric focusing - Congresses
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DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Analytical biochemistry --- Proteins --- Gel electrophoresis. --- Polyacrylamide gel electrophoresis. --- Analysis. --- Elektroforese. --- Nucleinezuren. --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- Amino acid sequence --- Rna --- GEL ELECTROPHORESIS --- NUCLEIC ACIDS --- ANALYSIS
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Now in a new second edition, this popular book includes a wealth of practical information on the much-used techniques of gel electrophoresis. Authoritative and completely current, the volume provides readers with an up-to-date, "how-to" guide to this growing field.
Nucleic acids --- Gel electrophoresis --- Acides nucléiques --- Electrophorèse en gel --- Analysis --- Analyse --- DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- -577.113 --- 57.088.3 --- 543.545 --- Elektroforese --- Polynucleotides --- Biomolecules --- Electrophoresis --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Methods and techniques for separation,fractionation and purification. --- Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- Gel electrophoresis. --- Basic Sciences. Chemistry --- Analysis. --- Analytical Chemistry --- Analytical Chemistry. --- 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- 577.113 Nucleic acids --- Acides nucléiques --- Electrophorèse en gel --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- 577.113 --- Methods and techniques for separation,fractionation and purification
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Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Electrophoresis, Polyacrylamide gel --- Immunoelectrophoresis --- Electrophoresis, Polyacrylamide Gel --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- isolation & purification --- Electrophoresis, Polyacrylamide Gel. --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Polyacrylamide gel electrophoresis. --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- Proteins. --- 577.112 --- 577.112 Proteins --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- 57.088 --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Analytical biochemistry --- Eiwitten. --- Elektroforese. --- methods --- laboratory manuals. --- Protein --- Electrophoresis, Polyacrylamide Gel - laboratory manuals --- Proteins - laboratory manuals
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This text presents the best methods, hints and tips on core procedures for running protein gels that will be widely applicable in the laboratory situation. The book's contributors are researchers writing primarily for researchers.
Proteins --- Gel electrophoresis. --- Gel electrophoresis --- Electrophoresis --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Electrochemical Techniques --- Chemistry Techniques, Analytical --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Electrophoresis, Polyacrylamide Gel --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Gene Products, Protein --- Gene Proteins --- Protein --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Electrochemical Technics --- Electrochemical Technic --- Electrochemical Technique --- Technic, Electrochemical --- Technics, Electrochemical --- Technique, Electrochemical --- Techniques, Electrochemical --- Electrophoreses --- Analysis. --- Analysis --- Electrophoresis.
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This specialist edition features key innovations in the science and engineering of new grinding processes, abrasives, tools, machines, and systems for a range of important industrial applications. Topics written by invited, internationally recognized authors review the advances and present results of research over a range of well-known grinding processes. A significant introductory review chapter explores innovations to achieve high productivity and very high precision in grinding. The reviewed applications range from grinding systems for very large lenses and reflectors, through to medium size grinding machine processes, and down to grinding very small components used in MEMS . Early research chapters explore the influence of grinding wheel topography on surface integrity and wheel wear. A novel chapter on abrasive processes also addresses the finishing of parts produced by additive manufacturing through mass finishing. Materials to be ground range from conventional engineering steels to aerospace materials, ceramics, and composites. The research findings highlight important new results for avoiding material sub-surface damage. The papers compiled in this book include references to many source publications which will be found invaluable for further research, such as new features introduced into control systems to improve process efficiency. The papers also reflect significant improvements and research findings relating to many aspects of grinding processes, including machines, materials, abrasives, wheel preparation, coolants, lubricants, and fluid delivery. Finally, a definitive chapter summarizes the optimal settings for high precision and the achievement of centerless grinding stability.
wheels --- safe operation --- precision --- lubrication --- surface roughness --- microgrinding --- high-performance dry grinding --- micro-grinding --- brittle hard materials --- glass --- micropencil grinding tools --- topography --- glass–ceramic --- cutting edge --- coolant delivery --- quality --- fusion --- materials --- metal additive manufacturing --- profile gear grinding --- grinding fluid --- coolant --- contact conditions --- metal cutting fluid --- grinding --- self-sharpening --- mass finishing --- process optimization --- centerless grinding --- grinding fluid nozzle --- laser --- productivity --- systems --- subsurface damages --- NC-form grinding --- sodium dodecyl sulfate --- grinding wheels --- processes --- jet breakup --- control --- removal rates --- wear modelling --- precision grinding --- optics manufacturing --- sensors --- bonding --- microstructures --- machines --- thermo-mechanical stress collective
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Water is indispensable to the functioning of most known life forms, and good water quality is essential to human health, social and economic development, and ecosystem functioning. Nonetheless, population growth has been leading to the degradation and depletion of fresh water resources. Under these circumstances, ensuring sufficient and safe water supplies for everyone is one of the Sustainable Development Goals (SDGs) set by the United Nations General Assembly in 2015 for the year 2030. For this goal to be achieved, the development and implementation of appropriate and efficient wastewater treatments that allow us to reduce water pollution is a major challenge.In view of the relevant contribution that polymers and polymeric materials may have in the conservation of the aquatic environment, namely by their application in wastewater treatment, original research and review papers on “Current trends and perspectives in the application of polymeric materials for wastewater treatment” were here brought together. For sure, this set of papers will be helpful and inspiring for readers interested in this topic.
Technology: general issues --- waste silk --- dopamine --- iron particles --- wastewater treatment --- activated carbon microsphere --- sodium lignosulfonate --- Cr(VI) --- adsorption --- modified polymeric resin --- t-butyl phosphate impregnation --- polymer based adsorbents --- dye adsorption --- response surface methodology --- nano-MgO --- structural modification --- permeability --- antifouling --- color rejection --- POME --- fluoroquinolones --- ultrasound radiation --- mesoporous carbon --- desirability function --- thermodynamics --- wastewater --- cost analysis --- ciprofloxacin --- Polystyrene nanocomposite --- modifications --- characterizations --- antibiotics --- emerging contaminants --- pharmaceuticals --- polymeric adsorbents --- magnetization --- silver nanoparticles --- microfiltration --- membranes --- biofouling --- sputtering --- magnetite --- co-precipitation method --- Rhodamine B --- sodium dodecyl sulfate --- selective adsorption --- dysprosium --- neodymium --- fabric adsorbent --- radiation --- graft polymerization --- molecular imprinting --- polymer --- sertraline --- cross-reactivity --- SSRI --- template --- sorbent --- n/a
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Water is indispensable to the functioning of most known life forms, and good water quality is essential to human health, social and economic development, and ecosystem functioning. Nonetheless, population growth has been leading to the degradation and depletion of fresh water resources. Under these circumstances, ensuring sufficient and safe water supplies for everyone is one of the Sustainable Development Goals (SDGs) set by the United Nations General Assembly in 2015 for the year 2030. For this goal to be achieved, the development and implementation of appropriate and efficient wastewater treatments that allow us to reduce water pollution is a major challenge.In view of the relevant contribution that polymers and polymeric materials may have in the conservation of the aquatic environment, namely by their application in wastewater treatment, original research and review papers on “Current trends and perspectives in the application of polymeric materials for wastewater treatment” were here brought together. For sure, this set of papers will be helpful and inspiring for readers interested in this topic.
waste silk --- dopamine --- iron particles --- wastewater treatment --- activated carbon microsphere --- sodium lignosulfonate --- Cr(VI) --- adsorption --- modified polymeric resin --- t-butyl phosphate impregnation --- polymer based adsorbents --- dye adsorption --- response surface methodology --- nano-MgO --- structural modification --- permeability --- antifouling --- color rejection --- POME --- fluoroquinolones --- ultrasound radiation --- mesoporous carbon --- desirability function --- thermodynamics --- wastewater --- cost analysis --- ciprofloxacin --- Polystyrene nanocomposite --- modifications --- characterizations --- antibiotics --- emerging contaminants --- pharmaceuticals --- polymeric adsorbents --- magnetization --- silver nanoparticles --- microfiltration --- membranes --- biofouling --- sputtering --- magnetite --- co-precipitation method --- Rhodamine B --- sodium dodecyl sulfate --- selective adsorption --- dysprosium --- neodymium --- fabric adsorbent --- radiation --- graft polymerization --- molecular imprinting --- polymer --- sertraline --- cross-reactivity --- SSRI --- template --- sorbent --- n/a
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