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Multidisciplinary resource for graduate studies and the biotechnology industry Knowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts, and new bioproducts from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes, and heart disease, or products for industry and agriculture, the science and technology of bioseparations are the keys to delivering these products in a purified form suitable for use by people. The methods, theory, and materials that reduce the science of bioseparations to practice, whether in the laboratory or the plant, are the subjects of Bioseparations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for: * Isocratic and gradient chromatography * Sedimentation, centrifugation, and filtration * Membrane systems * Precipitation and crystallization Topics addressed within this framework are: stationary phase selection; separations development; modeling of ion exchange, size exclusion, reversed phase, hydrophobic interaction, and affinity chromatography; the impact of regulatory issues on chromatography process design; organization of separation strategies into logical sequences of purification steps; and bridges between molecular biology, combinatorial methods, and separations science. A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research associates in the pharmaceutical, food, and life sciences industries.
General biochemistry --- Biotechnology --- size exclusion chromatography --- zware metalen --- ionen --- biodegradatie --- metaalionen --- biochemie --- biotechnologie --- afval
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size exclusion chromatography --- Organic chemistry --- fysicochemie --- organische chemie --- chromatografie --- Physicochemistry --- Macromolecules --- Fysicochemical separation methods --- Gel permeation chromatography --- Chemistry --- Physical Sciences & Mathematics --- Organic Chemistry --- Congresses --- Chromatographie sur gel. (Congrès) --- Gelchromatografie. (Congres)
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Gel permeation chromatography --- Chromatographie sur gel --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Gel permeation chromatography.
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The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses.These developments in princi
Gel permeation chromatography --- Chromatographie sur gel --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Gel permeation chromatography. --- Chromatographic analysis. --- Analysis, Chromatographic --- Chromatography --- Analytical chemistry --- Phase partition
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Gel permeation chromatography --- Chromatographie sur gel --- Congresses --- Congrès --- Congrès --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis
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Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Key Features * Evaluate and select columns with confidence for specific applications * Optimize separations and improve the ruggedness of analytical methods * Extend the service time of a column * Establish a quality-control program to ensure consistency in column performance * Avoid the expense of column damage or purchases that do not give the expected results
Gel permeation chromatography. --- Gel permeation chromatography --- Handbooks, manuals, etc. --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis
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Gel permeation chromatography --- Chromatographie sur gel --- Chromatography, Gel. --- 543.544 --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Chromatography, Size Exclusion --- Exclusion Chromatography --- Gel Chromatography --- Gel Permeation Chromatography --- Molecular Sieve Chromatography --- Chromatography, Exclusion --- Chromatography, Gel Permeation --- Chromatography, Molecular Sieve --- Gel Filtration --- Gel Filtration Chromatography --- Chromatography, Gel Filtration --- Exclusion Chromatography, Size --- Filtration Chromatography, Gel --- Filtration, Gel --- Sieve Chromatography, Molecular --- Size Exclusion Chromatography --- Chromatographic analysis. Chromatography --- 543.544 Chromatographic analysis. Chromatography --- Chromatography, Gel --- Gel permeation chromatography. --- Instrumentation
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General biochemistry --- Chromatography, Gel. --- 661.18 --- -Gel permeation chromatography --- -Molecular biochemistry --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatography, Size Exclusion --- Exclusion Chromatography --- Gel Chromatography --- Gel Permeation Chromatography --- Molecular Sieve Chromatography --- Chromatography, Exclusion --- Chromatography, Gel Permeation --- Chromatography, Molecular Sieve --- Gel Filtration --- Gel Filtration Chromatography --- Chromatography, Gel Filtration --- Exclusion Chromatography, Size --- Filtration Chromatography, Gel --- Filtration, Gel --- Sieve Chromatography, Molecular --- Size Exclusion Chromatography --- Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- -Chromatographic analysis. Chromatography --- 661.18 Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- -Chromatography, Gel permeation --- Molecular biology --- Gel permeation chromatography. --- Biochemistry --- Technique.
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Extracellular vesicles (EVs) are particles wrapped in a lipid bilayer membrane and are naturally released from cells. This kind of cargo vessel is a nanostructure that mainly transfers lipids, proteins, various nucleic acid fragments, and metabolic components to neighboring cells or distant parts of the body through the circulatory system. EVs are of great significance to the communication mechanism between cells. This book collects feature articles to enhance our understanding of the biological characteristics of EVs and their potential applications.
Medicine --- extracellular vesicle --- precision oncology --- cancer biomarker --- prostate cancer --- drug delivery --- extracellular vesicles --- lysosome --- nanocarriers --- ultrasound --- size exclusion chromatography --- differential ultracentrifugation --- head and neck squamous cell carcinoma (HNSCC) --- exosomes --- cancer --- biomarker --- diagnostic --- therapy --- liquid biopsy --- small extracellular vesicles (sEV) --- tumor-derived exosomes (TEX) --- melanoma cell-derived exosomes (MTEX) --- proteomics --- tumor microenvironment --- biomarkers --- ectosomes --- neoplasia --- microvesicles --- small extracellular vesicles --- isolation --- purification --- size-exclusion chromatography --- ultracentrifugation --- sucrose density cushion --- lymph node --- spleen --- solid tissue --- microvesicle --- exosome --- cancer therapeutic --- drug carrier --- flow cytometry --- immunophenotyping --- swarm detection --- tumor-associated macrophages --- macrophage polarization --- mannose receptor --- HIV-1 Nef --- glioblastoma --- microRNA --- immunoprecipitation --- CD44 --- human milk --- nutrient --- microbiota --- microRNAs --- nanocommunicator --- diagnostic biomarker --- drug delivery vehicle --- personalized cancer immunotherapy --- therapeutic agents --- cell-to-cell communication --- ionising radiation --- non-targeted effects --- signalling --- imaging flow cytometry --- biomarker reservoirs --- cancer diagnostics --- disease monitoring --- large EVs --- ovarian cancer cells --- ES-2 --- OAW-42 --- adipose tissue origin mesenchymal stem cells --- n/a
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