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004.453 --- PLC programmable logic controllers --- automatisering --- bussystemen --- combinatorische schakelingen --- digitale techniek --- handboeken --- informatieverwerking --- meettechnieken --- oefeningen --- powerpoint --- programmeertechnologie --- sequentie-analyse --- programmabesturingsprogrammatuur --- Contains audio-visual material --- Informatica --- Elektrische installaties --- Toegepaste informatica --- 3e graad secundair onderwijs --- Technisch secundair onderwijs
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The structure of a protein is essential for its function. Therefore, a variety of methods for analyzing protein structure have been developed recently; the most advanced and sensitive techniques have been selected for this manual. The presented procedures are well established and have been found useful in many laboratories; they are easy to perform and thus also suitable for beginners. Enclosed are protocols on the separation of proteins and peptides, manual and automated microsequencing, electrophoresis and blotting, analysis of amino acids, identification of cysteine residues and lipids as well as on mass spectrometry crystallization of macromolecules.
proteins --- peptides --- Chromatography --- genetic engineering --- Electrophoresis --- Amino acids --- Identification --- identification --- Spectrometry --- recombination --- Crystallization --- HPLC --- 543.64 --- 57.085 --- 612.08 --- massaspectrometrie --- sequentie-analyse --- Proteins --- Structure --- Laboratory manuals. --- identification. --- 57.088 --- aminozuren --- analytische chemie (scheikunde) --- biochemie --- biologie --- blotting --- elektroforese --- HPLC high performance liquid chromatography --- laboratoriumbiochemie --- peptiden --- proteïnen --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Structure&delete& --- Laboratory manuals --- Biochemistry. --- Cell biology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Macromolecule
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biotechnologie --- Biotechnology --- pharmacology --- farmacologie --- Pharmacognosy --- Pharmacosynthesis. Pharmacochemistry --- Pharmacology. Therapy --- 57.08 --- 576.3 --- 579.6 --- 579.63 --- 579.66 --- ELISA enzyme linked immunosorbent assays --- PCR polymerase chain reaction --- absorptie --- antistoffen --- biochemie --- biochemische ingenieurstechnieken --- biofarmacie --- biologie --- blotting --- celfysiologie --- centrifugeren --- chromatografie --- downstream processing --- elektroforese --- farmacokinetica --- filtratie --- geneesmiddelen --- genen --- genetische manipulatie --- gentherapie --- hormonen --- hybridisatie --- immunoassays --- lever --- massaspectrometrie --- microbiologie --- moleculaire biologie --- mutagenese --- nieren --- procestechnologie --- proteïnen --- recombinant DNA --- sequentie-analyse --- transcriptie --- translatie --- vaccins --- voeding --- biologische technieken, experimentele methoden en uitrusting in het algemeen --- toegepaste microbiologie --- hygiënische microbiologie --- industriële en chemische microbiologie --- biogeneesmiddelen --- interferon --- vaccinatie
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