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 This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Pseudomonas aeruginosa

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Emerging antibiotic resistance of bacteria is driving us to catastrophic failure of the healthcare system. Pseudomonas aeruginosa is one such pathogen that is responsible for causing nosocomial infections, urinary tract infections, cystic fibrosis, otitis media, and infections of wounds and burns. P. aeruginosa is a critical pathogen as defined by the World Health Organization (WHO) due to its intrinsic and adaptive competence to defy antibiotics and persist as a superbug. This book covers a wide array of subjects relevant to bacterial biofilms specifically focusing on P. aeruginosa and associated infections. It provides readers with a clear and comprehensive overview of biofilm formation and associated detrimental impacts. In addition, this book also examines topics related to biosynthesis virulence factors by P. aeruginosa to facilitate biofilm formation, antibiotic resistance mechanisms, and infections.

Pseudomonas aeruginosa infections.

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Pseudomonas aeruginosa, though unfamiliar as an aggressive invader, has gained importance in the scientific community due to its association with cystic fibrosis (CF) and its ability to construct biofilms resilient to host defense. The chronic nature of CF allows this bacterium to colonize, adapt, and evolve at its own pace, thereby causing further complications in CF patients. With its huge genetic repertoire and plasticity of the genome, P. aeruginosa has been able to alter its contents by way of deletions, insertions, inversions, and so on. Therefore scientists and researchers are eager to study this bacterium in diverse and unusual niches. Written by experts from around the world, this book describes and discusses the various mechanisms of adaptation and evolution displayed by P. aeruginosa.

Veterinary medicine. --- Pseudomonas aeruginosa.

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Emerging antibiotic resistance of bacteria is driving us to catastrophic failure of the healthcare system. Pseudomonas aeruginosa is one such pathogen that is responsible for causing nosocomial infections, urinary tract infections, cystic fibrosis, otitis media, and infections of wounds and burns. P. aeruginosa is a critical pathogen as defined by the World Health Organization (WHO) due to its intrinsic and adaptive competence to defy antibiotics and persist as a superbug. This book covers a wide array of subjects relevant to bacterial biofilms specifically focusing on P. aeruginosa and associated infections. It provides readers with a clear and comprehensive overview of biofilm formation and associated detrimental impacts. In addition, this book also examines topics related to biosynthesis virulence factors by P. aeruginosa to facilitate biofilm formation, antibiotic resistance mechanisms, and infections.

Pseudomonas aeruginosa infections.

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Pseudomonas aeruginosa is one of the most commonly isolated bacterial species in clinical microbiology laboratories and it causes infections in hospitalized patients with strains resistant to many antimicrobials. In addition, it can form a biofilm especially in cystic fibrosis patients and patients with medical devices. Extracellular polymeric substances (EPS) or extracellular matrixes in biofilm prevent access of antimicrobial agents, disinfectants, and host-produced immune responses. Antimicrobial resistance in Pseudomonas aeruginosa is multifactorial and can be due to mutation, transfer of genes by plasmids, or transposons, among other causes. Production of β-lactamases causes resistance to β-lactam drugs including Carbapenems.Against this background of emergence of antibiotic resistance, this book studies different types of β-lactamases routinely produced by Pseudomonas aeruginosa strains in clinical microbiology laboratories. It shows that, although molecular methods like Polymerase Chain Reaction (PCR) are the gold standard for detection of different types of β-lactamases, they are very costly and require expertise.

Pseudomonas aeruginosa infections.