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Microarrays emerges more and more to be an inevitable tool for molecular biology research. Indeed, the technological progresses of these few years make possible cells whole genome studies in a single experiment. It explains why genes-expression profiling studies are more frequently used in medical research as well as in the clinical reality.
In 2006, some researchers form the Massachusset Institute of Technology described a new microarray application : the Connectivity Map. It consists in a collection of gene-expression profiles that allows to connect biological process, diseases and small molecules.
This innovating microarray application held our mind. It’s why we decided to learn more about the fonctionnality and the uses of this tool. So we used the data from some UCL researchers to improve the Connectivity Map Les microarrays s’imposent de plus en plus comme un outil incontournable pour la recherche en biologie moléculaire. En effet, avec les progrès technologiques de ces dernières années, il est désormais possible d’étudier l’expression de l’ensemble des gènes d’une lignée cellulaire en une seule manipulation. C’est ainsi que les études de profils d’expression génomique prennent une place grandissante dans la recherche médicale ainsi que dans la réalité clinique.
En 2006, des chercheurs du Massachusset Institute of Technology ont décrit une nouvelle application de ces microarrays : le Connectivity Map. Il s’agit d’une banque de données permettant de relier des états biologiques et des molécules pharmacologiques, en se basant sur les profils d’expression génomique.
Cette utilisation innovante des microarrays a retenu toute notre attention. C’est pourquoi nous avons essayé de comprendre le principe de fonctionnement et les intérêts de cet outil. Des expériences effectuées par des chercheurs de l’UCL nous ont servi de base afin de tester ce Connectivity Map

Oligonucleotide Array Sequence Analysis --- Pharmacogenetics

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OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS --- GENE EXPRESSION PROFILING --- MICROCHEMISTRY --- METHODS

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DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. Such biochips enable the use of advanced fabrication, detection, and data mining technologies that allow data gathering at an unprecedented rate. The first chapter is an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips. The next two chapters describe the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. Chapter 4 describes assay optimization for enzymatic assays and chapter 5 antisense scanning arrays. This is followed by a chapter on the manufacture of molecular arrays using ink-jet printing technology. Chapter 7 describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics. Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology in pharmacogenomics. This Practical Approach book is a comprehensive overview of the new and expanding field of DNA microarray technology and will be invaluable to any researcher interested in the use of biochips.

micro-elektronica --- Molecular biology --- DNA (deoxyribonucleic acid) --- moleculaire biologie --- Puces à ADN --- Polymorphisme --- Polymorphism --- DNA microarrays. --- Escherichia coli --- Saccharomyces cerevisiae --- ADN --- DNA --- ARN --- RNA --- gene expression --- Puces à ADN --- DNA. --- RNA. --- bio-informatica --- klonen --- parallel processing --- oligonucleotides --- microarrays --- enzymen --- DNA microarrays --- Oligonucleotide Array Sequence Analysis --- 575.3 --- 575.3 Molecular genetics --- Molecular genetics --- DNA Arrays --- DNA Chips --- DNA Microchips --- Gene Chips --- Oligodeoxyribonucleotide Array Sequence Analysis --- Oligonucleotide Microarrays --- Sequence Analysis, Oligonucleotide Array --- cDNA Arrays --- DNA Microarrays --- Oligonucleotide Arrays --- cDNA Microarrays --- Array, DNA --- Array, Oligonucleotide --- Array, cDNA --- Arrays, DNA --- Arrays, Oligonucleotide --- Arrays, cDNA --- Chip, DNA --- Chip, Gene --- Chips, DNA --- Chips, Gene --- DNA Array --- DNA Chip --- DNA Microarray --- DNA Microchip --- Gene Chip --- Microarray, DNA --- Microarray, Oligonucleotide --- Microarray, cDNA --- Microarrays, DNA --- Microarrays, Oligonucleotide --- Microarrays, cDNA --- Microchip, DNA --- Microchips, DNA --- Oligonucleotide Array --- Oligonucleotide Microarray --- cDNA Array --- cDNA Microarray --- Oligonucleotide Probes --- Combinatorial Chemistry Techniques --- Gene Expression Profiling --- DNA biochips --- Biochips --- Immobilized nucleic acids --- instrumentation --- Gene Expression Microarray Analysis

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In situ hybridization --- Laboratory manuals. --- In situ hybridization - Laboratory manuals. --- Oligonucleotide probes

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DNA microarrays. --- Oligonucleotide Array Sequence Analysis --- Data Interpretation, Statistical. --- Gene Expression Profiling --- Research Design.