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Biological techniques --- Molecular biology --- Biotechnology --- Molecular cloning --- Recombinant DNA --- Genetic engineering --- Clonage moléculaire --- ADN recombinant --- Génie génétique --- DNA, Recombinant --- Cloning, Molecular --- methods --- 575.113.1 --- #WSCH:MONO --- methods. --- Inheritance information. Nature of gene. Genetic code --- 575.113.1 Inheritance information. Nature of gene. Genetic code --- Clonage moléculaire --- Génie génétique --- Molecular cloning. --- DNA. --- DNA --- Cloning, Molecular - methods --- Bacteria --- Eukaryotic cells --- Genetic recombination --- Mutation --- Nuclease, restriction endodeoxyribo --- -Plant --- Plant --- Plasmids --- Prokaryotic cells --- Recombination, genetic --- Yeasts

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DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.

DNA-protein interactions --- DNA-protein interactions. --- Electronic books. -- local. --- Protein binding. --- Nuclease Protection Assays --- Proteins --- Genetic Techniques --- Amino Acids, Peptides, and Proteins --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- DNA Footprinting --- DNA-Binding Proteins --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology.

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Ornamental crops account for more than US $42 billion globally. With the exception of few floral species, limited genetic, genomic, and breeding information is publicly available, owing to the fact that the majority of breeding work is performed by the private sector. Public research programs are increasingly participating in ornamental cultivar development and genetic studies. With lower sequencing costs, genomic information of non-model species including ornamental crops is continuously becoming available. Ornamental breeding utilizes a wide array of breeding strategies ranging from traditional crossing and selection methods to the use of next-generation sequencing in genomics and transcriptomics for gene identification and trait development. A continuing search of new species for the ornamentals industry has resulted in the utilization of tools that increase diversity and in the development of alternative methods for obtaining new crops by achieving interspecific and intergeneric crosses. This Special Issue aimed to present papers on new breeding methods, novel cultivars and species entering the ornamental industry, the identification of genes conferring novel traits, technological developments in ornamentals research, and the use of next-generation sequencing to improve ornamental plants.

Technology: general issues --- Biotechnology --- Acer buergerianum --- Acer ginnala --- Acer platanoides --- Acer tataricum ssp. ginnala --- ploidy manipulation --- sterility --- Hydrangea macrophylla --- SSR --- SNP --- linkage map --- flower senescence --- nuclease --- nutrient deficiency --- petals --- programmed cell death --- virus induced gene silencing --- bolting --- cut flower --- germinal pore --- ornamental plant --- polyploidy periclinal chimera --- Plumbaginaceae --- polyploidy breeding --- cold hardiness --- LT50 --- sucrose --- oligosaccharides --- soluble sugar metabolism --- gene expression --- breeding --- geophytes --- interspecific cross --- plant hormone treatment --- pollen-pistil interaction --- pre-zygotic barrier --- Ranunculaceae --- n/a

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Ornamental crops account for more than US $42 billion globally. With the exception of few floral species, limited genetic, genomic, and breeding information is publicly available, owing to the fact that the majority of breeding work is performed by the private sector. Public research programs are increasingly participating in ornamental cultivar development and genetic studies. With lower sequencing costs, genomic information of non-model species including ornamental crops is continuously becoming available. Ornamental breeding utilizes a wide array of breeding strategies ranging from traditional crossing and selection methods to the use of next-generation sequencing in genomics and transcriptomics for gene identification and trait development. A continuing search of new species for the ornamentals industry has resulted in the utilization of tools that increase diversity and in the development of alternative methods for obtaining new crops by achieving interspecific and intergeneric crosses. This Special Issue aimed to present papers on new breeding methods, novel cultivars and species entering the ornamental industry, the identification of genes conferring novel traits, technological developments in ornamentals research, and the use of next-generation sequencing to improve ornamental plants.

Technology: general issues --- Biotechnology --- Acer buergerianum --- Acer ginnala --- Acer platanoides --- Acer tataricum ssp. ginnala --- ploidy manipulation --- sterility --- Hydrangea macrophylla --- SSR --- SNP --- linkage map --- flower senescence --- nuclease --- nutrient deficiency --- petals --- programmed cell death --- virus induced gene silencing --- bolting --- cut flower --- germinal pore --- ornamental plant --- polyploidy periclinal chimera --- Plumbaginaceae --- polyploidy breeding --- cold hardiness --- LT50 --- sucrose --- oligosaccharides --- soluble sugar metabolism --- gene expression --- breeding --- geophytes --- interspecific cross --- plant hormone treatment --- pollen-pistil interaction --- pre-zygotic barrier --- Ranunculaceae

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Polypharmacy is a necessary and important aspect of drug treatment; however, it becomes a challenge when the medication risks outweigh the benefits for an individual patient. Drug–drug interactions and the introduction of prescribing cascades are common features of polypharmacy, which can lead to ineffectiveness and increased risk of adverse drug reactions (ADR). Genes encoding CYP450 isozymes and other drug-related biomarkers have attracted considerable attention as targets for pharmacogenetic (PGx) testing due to their impact on drug metabolism and response. This Special Issue is devoted to explore the status and initiatives taken to circumvent ineffectiveness and to improve medication safety for polypharmacy patients. Specific areas include drug–drug interactions and consequences thereof in therapeutic management, including PK- and PD-profiling; the application of PGx-based guidance and/or decision tools for drug–gene and drug–drug gene interactions; medication reviews; development and application of deprescribing tools; and drivers and barriers to overcome for successful implementation in the healthcare system.

Medicine --- Pharmaceutical industries --- acute kidney injury --- early biomarker --- plasma neutrophil gelatinase-associated lipocalin --- soluble urokinase plasminogen activator receptor --- medication optimization --- older patients --- emergency department --- multimorbidity --- polypharmacy --- potentially inappropriate medication use --- older adults --- prevalence --- determinants --- chronic --- outpatient --- 2019 Beers criteria --- Ethiopia --- pharmacogenomics --- persons with diabetes --- drug–drug interactions --- drug–gene interactions --- cytochrome P450 --- SLCO1B1 --- drug interaction checkers --- adverse drug reactions --- pharmacogenetics --- personalized medicine --- phenprocoumon --- DOACs --- bleeding --- thromboembolism --- HLA --- drug hypersensitivity --- abacavir --- allopurinol --- flucloxacillin --- antiepileptic drugs --- cost-effectiveness --- shared medication record --- medication reconciliation --- drug information service --- hospital pharmacy service --- electronic prescribing --- electronic medical record --- clinical pharmacist --- CYP2D6 --- CYP2D7P --- CYP2D8P --- copy number variation --- CNV --- genotyping --- 5’nuclease assay --- HRM --- high resolution melting --- drug metabolization --- extracellular vesicles --- exosomes --- microvesicles --- pharmacogene expression --- medication review --- deprescriptions --- quality of life --- aged --- aged, 80 and over --- nursing homes --- deprescribing --- medication-based risk score --- health outcomes --- cytochromes --- CYP1A2 --- adverse drug reaction --- antipsychotics --- olanzapine --- clozapine --- loxapine --- children --- youth --- digital decision-support --- health services research --- general practice --- process evaluation --- antidepressants --- utility --- population-based --- appropriateness --- medication adherence --- digital health --- acute kidney injury --- early biomarker --- plasma neutrophil gelatinase-associated lipocalin --- soluble urokinase plasminogen activator receptor --- medication optimization --- older patients --- emergency department --- multimorbidity --- polypharmacy --- potentially inappropriate medication use --- older adults --- prevalence --- determinants --- chronic --- outpatient --- 2019 Beers criteria --- Ethiopia --- pharmacogenomics --- persons with diabetes --- drug–drug interactions --- drug–gene interactions --- cytochrome P450 --- SLCO1B1 --- drug interaction checkers --- adverse drug reactions --- pharmacogenetics --- personalized medicine --- phenprocoumon --- DOACs --- bleeding --- thromboembolism --- HLA --- drug hypersensitivity --- abacavir --- allopurinol --- flucloxacillin --- antiepileptic drugs --- cost-effectiveness --- shared medication record --- medication reconciliation --- drug information service --- hospital pharmacy service --- electronic prescribing --- electronic medical record --- clinical pharmacist --- CYP2D6 --- CYP2D7P --- CYP2D8P --- copy number variation --- CNV --- genotyping --- 5’nuclease assay --- HRM --- high resolution melting --- drug metabolization --- extracellular vesicles --- exosomes --- microvesicles --- pharmacogene expression --- medication review --- deprescriptions --- quality of life --- aged --- aged, 80 and over --- nursing homes --- deprescribing --- medication-based risk score --- health outcomes --- cytochromes --- CYP1A2 --- adverse drug reaction --- antipsychotics --- olanzapine --- clozapine --- loxapine --- children --- youth --- digital decision-support --- health services research --- general practice --- process evaluation --- antidepressants --- utility --- population-based --- appropriateness --- medication adherence --- digital health