Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Cassava root rot disease was first reported in Kinshasa and Kongo Central in the Democratic Republic of Congo (Mahungu et al., 2003). Currently, no study has been able to find the etiology of the symptoms by identifying one or more responsible pathogen(s).
The main objective of this study was to contribute to the etiology of cassava root necrosis disease in western DR Congo. To this end, the first specific objective was to reanalyze HTS data from (Bakelana et al., 2020) to identify viruses present in the samples and the second to isolate symptomatic plants and characterize fungal microorganisms potentially involved in CRND.
The results obtained after rechecking the HTS data of the Bakelana team showed no contigs of viral origin. Moreover, some contigs did not show homologies in the NCBI database, nor did they show any prediction of their ORF with a start codon and a stop codon.
A total of 20 pure strains of fungi were isolated. One from the Mputa variety and 19 from the Mvuazi variety respectively sensitive and tolerant to CRND). The results obtained after morphological and molecular study identified 17 isolates as strains belonging to Fusarium solani and two isolates belonging to the genus Trichoderma.
The phylogenetic analyses consisted in comparing, after multiple alignments in the cristalW program integrated in MEGA X 11.0.13, ITS sequences of isolates obtained after PCR and sequencing with those of species contained in the NCBI nt database using the BLASTn A maximum likelihood phylogenetic tree reconstructed according to the GTR + GI model based on the alignment performed previously confirmed 16 isolates close to Fusarium solani and 2 isolates close to the genus Trichoderma. Two isolates could not be molecularly characterized because of their poor quality. La maladie des nécroses racinaires de manioc a été reportée pour la première fois à Kinshasa et au Kongo Central en République Démocratique du Congo (Mahungu et al., 2003). Actuellement, aucune étude n’est parvenue à trouver l’étiologie des symptômes en identifiant un ou plusieurs agent(s) pathogène(s) responsable(s).
L’objectif principal de cette étude était la contribution à l’étiologie de la maladie des nécroses racinaires de manioc à l’ouest de la RD Congo. Pour ce faire, le premier objectif spécifique a consisté à réanalyser des données HTS de (Bakelana et al., 2020) en vue d’identifier des virus présents dans les échantillons et le second à isoler de plantes symptomatiques et à caractériser des microorganismes fongiques potentiellement impliqués dans la CRND.
Les résultats obtenus après re-vérification des données HTS de l’équipe de Bakelana n’ont montré aucun contig d’origine virale. Par ailleurs, certains contigs n’ont pas présenté d’homologies dans la base des données NCBI, ni de prédiction d’ORF avec codon start et un codon stop. 
Au total, vingt souches pures de champignons ont été isolées. Un sur la variété Mputa et 19 sur la variété Mvuazi respectivement sensible et tolérante à la CRND. Les résultats obtenus après l’étude morphologique et moléculaire ont identifié 17 isolats comme de souches appartenant au Fusarium solani et deux isolats appartenant au genre Trichoderma. 
Les analyses phylogénétiques effectuées consistaient à comparer, après alignements multiples dans le programme cristalW intégré dans MEGA X 11.0.13, des séquences ITS des isolats obtenus après PCR et séquençage avec celles des espèces contenues dans la base des données nt du NCBI en utilisant le programme BLASTn. 
Un arbre phylogénétique à vraisemblance maximale (maximum likelihood) reconstruit selon le modèle GTR + GI sur base de l’alignement réalisé précédemment a confirmé 16 isolats proches du Fusarium solani et 2 isolats proches du genre Trichoderma . Deux isolats n’ont pu être caractérisé sur le plan moléculaire car étant de mauvaise qualité.

Cassava, fungus, CRND, isolation, molecular characterization, bioinformatics --- Manioc, champignon, CRND, isolement, caractérisation moléculaire, bio-informatique --- Sciences du vivant > Agriculture & agronomie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Numerous pathogens affect animal health and wellbeing and production efficiency. These pathogens also have a considerable impact on social economics, food safety and security, and human health. Infectious diseases that originate from both domesticated animals and wildlife represent one of the greatest threats to human health. Recent studies show that domesticated species harbor approximately 84 times more zoonotic viruses than wild species. Eight of the top 10 mammalian species with the highest number of zoonotic viruses are domestic, such as pigs, cattle, and horses. Many animal parasites are also zoonotic, constituting an additional burden on human health. Furthermore, the rapid emergence and spread of drug-resistant pathogen strains pose new threats to animal and human health. Climate changes will undoubtedly alter the interactions between animals and between animals and humans, which will have a huge impact on the transmission rate of existing pathogens and the emergence of new pathogens or the reemergence of old pathogens. In this special collection, interactions of all major pathogen types, including viruses, bacteria, mites and flies, protozoans, and helminths, and their hosts, such as wild and companion animals and livestock species, are discussed. Further, anthelmintic activities of natural products are evaluated. The relevance and utility of cutting-edge tools, such as immunology, genomics and genetics, microbiome studies and metabolomics, and molecular epidemiology, in dissecting host-pathogen interactions are also discussed. This special collection provides a broad knowledge base that encourages dialogue across a wide distribution of the research community in veterinary microbiology and parasitology.

Medicine --- dietary treatments --- plant bioactive compounds --- egg counts --- UHRMS --- Haemonchus contortus --- Crotalus ravus --- Crotalus triseriatus --- venom --- antibacterial activity --- Pseudomonas aeruginosa --- hemolytic activity --- maedi-visna virus --- ovine progressive pneumonia --- small ruminant lentivirus --- dairy sheep --- horse --- colic --- gastrointestinal disease --- Strongylus vulgaris --- Anoplocephala perfoliata --- cyathostominae --- D. gallinae --- hematophagous ectoparasite --- poultry red mite --- antibody titre --- lymphocyte subpopulation --- Sarcocystis spp. --- COX1 --- Camelus dromedarius --- post-mortem microbiology --- veterinary forensic pathology --- sudden death --- young dogs --- bovine digital dermatitis --- cattle lameness --- microbiome --- Treponema spp --- Ancylostoma ceylanicum --- community dogs --- ITS region --- cox1 --- Thailand --- population diversity --- Sarcoptes scabiei --- host-parasite interaction --- molecular characterization --- lipid profile --- antioxidant --- rodent --- helminth --- cestode --- trematode --- nematode --- Middle East --- meta-analysis --- Brugia pahangi --- Dirofilaria immitis --- PCR-RFLP --- spatial distribution --- altitude --- myiasis --- prevalence --- Oestrus ovis --- mtCOI --- sheep --- goats --- Saudi Arabia --- anoplocephala perfoliata --- equine --- gut microbiome --- volatile organic compounds (VOCs) --- omics integration --- tick-borne fever --- Anaplasma phagocytophilum-like 1 --- small ruminant