Narrow your search
Listing 1 - 5 of 5
Sort by
Affinity chromatography
Author:
ISBN: 0444416056 9786611772413 1281772410 0080858120 9780444416056 9780080858128 9781281772411 6611772413 Year: 1978 Volume: 12 Publisher: Amsterdam New York New York Elsevier Scientific Pub. Co. Distributors for the U.S. and Canada, Elsevier North-Holland

Immobilized affinity ligand techniques
Authors: --- ---
ISBN: 0123423309 Year: 1992 Publisher: San Diego (Calif.) : Academic press,

Loading...
Export citation

Choose an application

Bookmark

Abstract

This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Key Features * Matrix activation * Ligand immobilization * Binding and elution of target molecules * Enzyme catalysis on solid supports * Analytical affinity chromatography * Isolation/purification of antibodies * Preparation of antibody fragments * Immunoaffinity chromatography * Immobilization of nucleic acids * Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: * Matrices * Spacers * Methods of activation and coupling Background information and insights on: * Affinity interactions * The ease and power of affinity chromatography * Attaching molecules to insoluble supports * Matrices currently in use * Over 20 methods of activation * Spacers * Extensive References


Book
Fundamentals of SARS-CoV-2 Biosensors
Authors: --- ---
ISBN: 3036557687 3036557679 Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

Loading...
Export citation

Choose an application

Bookmark

Abstract

COVID-19 diagnostic strategies based on advanced techniques are currently essential topics of interest, with crucial roles in scientific research. This book integrates fundamental concepts and critical analyses that explore the progress of modern methods for the detection of SARS-CoV-2.

Listing 1 - 5 of 5
Sort by