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A single source of authoritative information on all aspects of the practice of modern liquid chromatography suitable for advanced students and professionals working in a laboratory or managerial capacity Chapters written by authoritative and visionary experts in the field provide an overview and focused treatment of a single topicComprehensive coverage of modern liquid chromatography from theory, to methods, to selected applicationsThorough selected references and tables with commonly used data to facilitate research, practical work, comparison of result
Chemistry. --- Hydrophilic interaction liquid chromatography. --- Liquid chromatography. --- Liquid chromatography --- Chemistry, Analytic --- Chemistry --- Human Anatomy & Physiology --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Animal Biochemistry --- Analytical Chemistry --- Chromatographic analysis. --- Equipment and supplies. --- Analysis, Chromatographic --- Chromatography --- Liquid-liquid partition chromatography --- Phase partition --- Chromatographic analysis --- Analytical chemistry --- Chemistry, Analytic. --- Analytical chemistry.
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In this Topical Collection, ten articles (one review and nine research articles) are published in a time span of 2021–2022. All articles are written by experts in the field of Separation Techniques who were invited to contribute to the presentation of the current status in separation science. The authors were invited to answer the questions: What is the state-of-the-art in Separation Sciences? What advances have been reported recently? Last but not least, what are the future perspectives? The Editor and authors hope that the readers will find valuable information in the topic.
Research & information: general --- Chemistry --- protein-based chiral stationary phase --- alpha 1-acid glycoprotein --- chiral recognition mechanism --- molecular docking --- proteomics --- high-performance liquid chromatography --- mass spectrometry --- gout --- uric acid --- Salvia miltiorrhiza --- HPLC-MS/MS --- pharmacokinetics --- wine-processed --- hydrophilic interaction liquid chromatography --- chromatography --- oligopeptides --- acetyl hexapeptide-8 --- acetyl hexapeptide-3 --- Argireline --- cosmetics --- SARS-CoV-2 --- virus particles --- air filtering --- aerosols --- probability distributions --- rotational particle separator --- air separation --- steam methane reforming --- water gas shift --- alternative fluids --- gas turbine --- Senecio anteuphorbium --- response factors --- true quantitation --- allelopathy --- herbicide --- chlorpropham --- potato --- μQuEChERS/UHPLC-PDA --- validation --- cooking --- methyl nicotinate --- methyl salicylate --- ethyl salicylate --- 2-hydroxyethyl salicylate --- pain relief spray --- Tulasnellaceae sp. --- Gymnadenia orchidis --- mdium-pressure liquid chromatography --- reversed-phase liquid chromatography --- ergosterol
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Food safety is a matter of great significance for the global population. Therefore, researchers have been developing and validating analytical methods to extract, separate and quantitate a variety of hazardous and nutritional analytes in various food commodities. Due to the complexity of food components, a suitable pretreatment method is required to eliminate matrix effects and lower the detection limit. Afterward, chromatography and mass spectrometry are powerful tools in the guarantee of food safety and quality. This book is the reprint of a Special Issue of Separations, “Advances of Accurate Quantification Methods in Food Analysis”, and provides an overview of recent trends in food analytical methods. Both novel sample pretreatment and detection techniques are covered, with the aim of accurate quantification. This Special Issue received nine contributions that covered the latest analytical methods, and focused on pesticides, mycotoxin, antibiotics, metal ions, organic selenium and anthocyanins.
Research & information: general --- Chemistry --- Analytical chemistry --- extract-dilute-shoot --- fosetyl-aluminum --- hydrophilic interaction liquid chromatography --- wheat flour --- broiler tissue --- orbitrap high resolution mass spectrometry --- mycotoxins --- rapid screening --- solid phase extraction --- selenium --- speciation --- enzymatic hydrolysis --- HPLC-ICP-MS --- egg --- aquaculture products --- antibiotic residues --- liquid chromatography tandem mass spectrometry (LC/MS/MS) --- sample pre-treatment --- matrix effects --- QuEChERS --- gas-chromatography high resolution mass spectrometry --- liquid-chromatography high resolution mass spectrometry --- pesticide residues --- cottonseed hull --- raw milk 1 --- pesticides 2 --- screening 3 --- QuEChERS 4 --- high-throughput 5 --- mini solid-phase extraction --- multiclass pesticide residues --- tobacco --- gas chromatography quadrupole time-of-flight mass spectrometry --- arsenic speciation --- rice flour --- ultrasound-assisted extraction --- anthocyanins --- grapes --- UPLC-Q-TOFMS --- QAMS --- n/a
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Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees.
Medicine --- metabolomics --- sample preparation --- hydrophilic interaction liquid chromatography --- ion mobility spectrometry --- high resolution mass spectrometry --- design of experiments --- AMOPLS --- metabonomics --- metabolic profiling --- NMR --- nuclear magnetic resonance spectroscopy --- cell line --- human cell line --- MiaPaCa-2 --- Panc-1 --- AsPC-1 --- extracellular vesicles --- exosomes --- microvesicles --- biomarkers --- diagnostics --- metabolic pathways --- plant metabolomics --- forestry --- trees --- mass spectrometry --- metabolite extraction --- GC-MS --- LC-MS --- metadata standardization --- databases --- multicellular tumor spheroids --- metallodrugs --- oxaliplatin --- KP1339 --- method development --- IT-139 --- 20% FCS --- harvesting --- extraction --- metabolites --- normalization --- electromembrane extraction --- cardiovascular disease --- multi-segment injection --- capillary electrophoresis–mass spectrometry --- liquid chromatography–mass spectrometry --- plant natural products --- drug discovery --- liquid chromatography --- gas chromatography --- human milk --- metabolome --- sampling --- liquid chromatography–mass spectrometry (LC-MS) --- nuclear magnetic resonance (NMR) --- gas chromatography–mass spectrometry (GC-MS) --- capillary electrophoresis—mass spectrometry (CE-MS) --- high-resolution magic angle spinning --- microscopic samples --- lipidomics --- LC-MS/MS --- human plasma
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Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees.
metabolomics --- sample preparation --- hydrophilic interaction liquid chromatography --- ion mobility spectrometry --- high resolution mass spectrometry --- design of experiments --- AMOPLS --- metabonomics --- metabolic profiling --- NMR --- nuclear magnetic resonance spectroscopy --- cell line --- human cell line --- MiaPaCa-2 --- Panc-1 --- AsPC-1 --- extracellular vesicles --- exosomes --- microvesicles --- biomarkers --- diagnostics --- metabolic pathways --- plant metabolomics --- forestry --- trees --- mass spectrometry --- metabolite extraction --- GC-MS --- LC-MS --- metadata standardization --- databases --- multicellular tumor spheroids --- metallodrugs --- oxaliplatin --- KP1339 --- method development --- IT-139 --- 20% FCS --- harvesting --- extraction --- metabolites --- normalization --- electromembrane extraction --- cardiovascular disease --- multi-segment injection --- capillary electrophoresis–mass spectrometry --- liquid chromatography–mass spectrometry --- plant natural products --- drug discovery --- liquid chromatography --- gas chromatography --- human milk --- metabolome --- sampling --- liquid chromatography–mass spectrometry (LC-MS) --- nuclear magnetic resonance (NMR) --- gas chromatography–mass spectrometry (GC-MS) --- capillary electrophoresis—mass spectrometry (CE-MS) --- high-resolution magic angle spinning --- microscopic samples --- lipidomics --- LC-MS/MS --- human plasma
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