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An ever-increasing number of genes have been sequenced but are of unknown function. The ability to determine the location of gene products within the cell is a vital step towards understanding them. This guide provides practical advice.

Proteins --- Fluorescence microscopy. --- Microscopy --- Analysis. --- Green fluorescent protein. --- Immunofluorescence.

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Since scientists began experimenting with green fluorescent proteins in the middle of the 1990s, these proteins have become one of the most important tools available to researchers in modern medicine and biology. By using them to illuminate other proteins that were previously invisible even under microscope, scientists are now able to observe facets of disease that would have otherwise gone undetected. Green fluorescent proteins are a part of over three million experiments a year, and are invaluable for tasks such as tracking HIV, breeding bird flu-resistant chickens, and confirming the existe

Green fluorescent protein. --- GFP (Protein) --- Green jellyfish protein --- Fluorescent polymers --- Proteins

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Saccharomyces cerevisiae --- Saccharomyces cerevisiae --- protein synthesis --- protein synthesis --- genetic code --- genetic code --- genetic engineering --- genetic engineering --- Metabolism --- Metabolism --- Biologie cellulaire --- Green fluorescent protein --- Site d'encrage --- Biologie cellulaire --- Green fluorescent protein --- Site d'encrage

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Proteins --- Fluorescence microscopy. --- Green fluorescent protein. --- Immunofluorescence. --- Protéines --- Microscopie de fluorescence --- Protéine fluorescente verte --- Immunofluorescence --- Analysis. --- Analyse --- LABORATORY MANUALS --- FLUORESCENCE MICROSCOPY --- PROTEINS --- HANDBOOKS --- LOCALIZATION

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Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.

Analytical biochemistry --- Green fluorescent protein --- Protéine fluorescente verte --- Laboratory manuals. --- Manuels de laboratoire --- GFP (Protein) --- Green jellyfish protein --- Fluorescent polymers --- Proteins --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists