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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

Civil engineering, surveying & building --- Biotechnology --- synthetic biology --- genome engineering --- metabolic engineering --- evolution engineering --- cell-free systems --- bionanoengineering

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next-generation sequencing --- genome engineering --- imaging technology --- biotechnology --- cellular biology --- synthetic biology --- Cell Physiological Phenomena --- Cell Biology --- Cytology --- Cytology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cellular Biology --- Biologies, Cell --- Biologies, Cellular --- Biology, Cell --- Biology, Cellular --- Cell Biologies --- Cellular Biologies --- Cell Physiological Phenomenon --- Cell Physiological Process --- Physiology, Cell --- Cell Physiological Processes --- Cell Physiology --- Phenomena, Cell Physiological --- Phenomenon, Cell Physiological --- Physiological Process, Cell --- Physiological Processes, Cell --- Process, Cell Physiological --- Processes, Cell Physiological --- physiology

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Viruses are microscopic agents that exist worldwide and are present in humans, animals, plants, and other living organisms in which they can cause devastating diseases. However, the advances of biotechnology and next-generation sequencing technologies have accelerated novel virus discovery, identification, sequencing, and manipulation, showing that they present unique characteristics that place them as valuable tools for a wide variety of biotechnological applications. Many applications of viruses have been used for agricultural purposes, namely concerning plant breeding and plant protection. Nevertheless, it is interesting to mention that plants have also many advantages to be used in vaccine production, such as the low cost and low risks they entail, showing once more the versatility of the use of viruses in biotechnology. Although it will obviously never be ignored that viruses are responsible for devastating diseases, it is clear that the more they are studied, the more possibilities they offer to us. They are now on the front line of the most revolutionizing techniques in several fields, providing advances that would not be possible without their existence. In this book there are presented studies that demonstrate the work developed using viruses in biotechnology. These studies were brought by experts that focus on the development and applications of many viruses in several fields, such as agriculture, the pharmaceutical industry, and medicine.

Technology: general issues --- Bacteriophage --- Salmonella --- biocontrol --- comparative genomics --- phage diversity --- grapevine --- apple latent spherical virus vector --- virus-induced flowering --- reduced generation time --- breeding of grapevine --- virus elimination --- Newcastle disease virus --- reverse genetics --- vaccines --- infectious diseases --- cancer --- porcine epidemic diarrhea virus --- VLP --- chemokines --- pig --- vaccine --- SARS-CoV-2 --- COVID-19 --- phages --- CRISPR --- viruses --- prevention --- diagnosis --- treatment --- adeno-associated virus (AAV) vector --- jaagsiekte sheep retrovirus (JSRV) --- LTR --- enhancer --- transduction --- viral vaccines --- cancers --- COVID-19 vaccines --- self-replicating RNA vectors --- DNA-based vaccines --- RNA-based vaccines --- plant virus --- viroid --- viral vector --- virus-induced gene silencing (VIGS) --- CRISPR/Cas9 --- genome editing --- carotenoid biosynthesis --- circular RNA --- infectious bursal disease virus --- immunization --- recombinant Lactococcus lactis --- variant strain --- baculovirus --- insect cells --- bacmid --- Tn7 --- genome stability --- protein expression --- chikungunya virus --- VLPs --- bioreactor --- CRISPR/Cas systems --- viral vectors --- gene editing --- plant genome engineering --- viral resistance --- adeno-associated virus --- AAV --- cancer gene therapy --- prophage --- hydrothermal vent --- Hypnocyclicus thermotrophus --- lytic cassette --- Escherichia coli --- heterologous expression --- codon optimization --- codon harmonization --- expression vectors --- aspect ratio --- VNPs --- TMV --- PVX --- CPMV --- geminivirus --- theranostics --- CRISPR-cas9 --- clodronate --- macrophage --- gene therapy --- gene expression --- nanotechnology

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• Reference Manager
• EndNote
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Viruses are microscopic agents that exist worldwide and are present in humans, animals, plants, and other living organisms in which they can cause devastating diseases. However, the advances of biotechnology and next-generation sequencing technologies have accelerated novel virus discovery, identification, sequencing, and manipulation, showing that they present unique characteristics that place them as valuable tools for a wide variety of biotechnological applications. Many applications of viruses have been used for agricultural purposes, namely concerning plant breeding and plant protection. Nevertheless, it is interesting to mention that plants have also many advantages to be used in vaccine production, such as the low cost and low risks they entail, showing once more the versatility of the use of viruses in biotechnology. Although it will obviously never be ignored that viruses are responsible for devastating diseases, it is clear that the more they are studied, the more possibilities they offer to us. They are now on the front line of the most revolutionizing techniques in several fields, providing advances that would not be possible without their existence. In this book there are presented studies that demonstrate the work developed using viruses in biotechnology. These studies were brought by experts that focus on the development and applications of many viruses in several fields, such as agriculture, the pharmaceutical industry, and medicine.

Technology: general issues --- Bacteriophage --- Salmonella --- biocontrol --- comparative genomics --- phage diversity --- grapevine --- apple latent spherical virus vector --- virus-induced flowering --- reduced generation time --- breeding of grapevine --- virus elimination --- Newcastle disease virus --- reverse genetics --- vaccines --- infectious diseases --- cancer --- porcine epidemic diarrhea virus --- VLP --- chemokines --- pig --- vaccine --- SARS-CoV-2 --- COVID-19 --- phages --- CRISPR --- viruses --- prevention --- diagnosis --- treatment --- adeno-associated virus (AAV) vector --- jaagsiekte sheep retrovirus (JSRV) --- LTR --- enhancer --- transduction --- viral vaccines --- cancers --- COVID-19 vaccines --- self-replicating RNA vectors --- DNA-based vaccines --- RNA-based vaccines --- plant virus --- viroid --- viral vector --- virus-induced gene silencing (VIGS) --- CRISPR/Cas9 --- genome editing --- carotenoid biosynthesis --- circular RNA --- infectious bursal disease virus --- immunization --- recombinant Lactococcus lactis --- variant strain --- baculovirus --- insect cells --- bacmid --- Tn7 --- genome stability --- protein expression --- chikungunya virus --- VLPs --- bioreactor --- CRISPR/Cas systems --- viral vectors --- gene editing --- plant genome engineering --- viral resistance --- adeno-associated virus --- AAV --- cancer gene therapy --- prophage --- hydrothermal vent --- Hypnocyclicus thermotrophus --- lytic cassette --- Escherichia coli --- heterologous expression --- codon optimization --- codon harmonization --- expression vectors --- aspect ratio --- VNPs --- TMV --- PVX --- CPMV --- geminivirus --- theranostics --- CRISPR-cas9 --- clodronate --- macrophage --- gene therapy --- gene expression --- nanotechnology