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Catheter ablation is a major treatment for atrial tachycardias. Hereby, the precise monitoring of the lesion formation is an important success factor. This book presents computational, wet-lab, and clinical studies with the aim of evaluating the signal characteristics of the intracardiac electrograms (IEGMs) recorded around ablation lesions from different perspectives. The detailed analysis of the IEGMs can optimize the description of durable and complex lesions during the ablation procedure.
Akute Ablationsläsionen --- electrophysiology --- Computermodell --- intracardiac electrograms --- Intrakardiale Elektrogramme --- Elektrophysiologie --- computational modeling --- ex vivo experiments --- Ex vivo Experimente --- acute ablation lesions
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poultry --- Viroses --- Immunosuppression --- Pathogenicity --- Disease resistance --- Measurement --- blood --- lymphocytes --- In vitro experimentation --- Bursa fabricii --- Experimentation ex vivo --- Splenocyte --- Experimentation ex vivo --- Splenocyte
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The purpose this thesis is to study the effect of fermentable feed ingredients on the intestinal health of weaned piglets via an in vitro fermentation model combined with an ex vivo model on intestinal explants. This work will focus on two different pools of ingredients. The first pool will focus on chicory, it’s corresponding by-products and purified fractions. The same principle will be applied to the second pool that is composed out of oat and barley. All the feed ingredients are going to be tested by an in-vitro model combined with an ex-vivo model. The aim of those experiments is to determine whether barley, oat, chicory or by-products could have a close effect on intestinal health corresponding to their purified fraction. First, an in vitro fermentation will be performed using weaned piglets' feces. The aim is to measure the resulting gas production and to collect fermentation fluids. Based on these fermentation juices, we can measure the concentration in volatile fatty acids by HPLC but also estimate the relative amount of butyrogenic species by PCR. These fermentation juices will also be used during the ex vivo procedure. The ex vivo model will consist in inducing an inflammation (via LPS of E. Coli) in weaned piglets' intestinal explants and see if the applied fermentation juices are able reduce inflammation. For that, cytokines, tight junctions and mucins mRNA levels will be analysed by qRT-PCR. Based on the in vitro - ex vivo model analyses, we can thereafter select which fermentable feed ingredients are the most suitable for the in vivo trial on weaned piglets.
Fibers --- Intestinal health --- weaned piglets --- in vitro model --- ex vivo model --- Sciences du vivant > Productions animales & zootechnie
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The book "Green Synthesis of Nanomaterials and Their Biological Applications" present novel advances about the green synthesis of nanomaterials, as well as on the possible biological applications, including studies dealing with entomology, parasitology, biomedicine, and environmental research.
Medicine --- metamaterial --- multiple resonances --- biochemical sensing --- environment sensor --- net formulations --- stored product insects --- knockdown --- mortality --- long-term effect --- European grapevine moth --- green pesticide --- insect pest --- Integrated Pest Management --- Larvicide --- nano-insecticide --- Tortricidae --- hydatid cyst --- protoscoleces --- nanomedicine --- in vitro --- in vivo --- ex vivo --- zinc oxide --- nanoparticles --- black tea extract --- gallic acid --- green synthesis --- antioxidants --- antimicrobial activity --- silver nanoparticles --- Diospyros malabarica --- antibacterial --- anticancer --- catalyst --- 4-nitrophenol --- electromechanically --- tunability --- metamaterials --- multi-functionalities --- terahertz --- refraction index sensor --- metamaterial --- multiple resonances --- biochemical sensing --- environment sensor --- net formulations --- stored product insects --- knockdown --- mortality --- long-term effect --- European grapevine moth --- green pesticide --- insect pest --- Integrated Pest Management --- Larvicide --- nano-insecticide --- Tortricidae --- hydatid cyst --- protoscoleces --- nanomedicine --- in vitro --- in vivo --- ex vivo --- zinc oxide --- nanoparticles --- black tea extract --- gallic acid --- green synthesis --- antioxidants --- antimicrobial activity --- silver nanoparticles --- Diospyros malabarica --- antibacterial --- anticancer --- catalyst --- 4-nitrophenol --- electromechanically --- tunability --- metamaterials --- multi-functionalities --- terahertz --- refraction index sensor
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The book "Green Synthesis of Nanomaterials and Their Biological Applications" present novel advances about the green synthesis of nanomaterials, as well as on the possible biological applications, including studies dealing with entomology, parasitology, biomedicine, and environmental research.
Medicine --- metamaterial --- multiple resonances --- biochemical sensing --- environment sensor --- net formulations --- stored product insects --- knockdown --- mortality --- long-term effect --- European grapevine moth --- green pesticide --- insect pest --- Integrated Pest Management --- Larvicide --- nano-insecticide --- Tortricidae --- hydatid cyst --- protoscoleces --- nanomedicine --- in vitro --- in vivo --- ex vivo --- zinc oxide --- nanoparticles --- black tea extract --- gallic acid --- green synthesis --- antioxidants --- antimicrobial activity --- silver nanoparticles --- Diospyros malabarica --- antibacterial --- anticancer --- catalyst --- 4-nitrophenol --- electromechanically --- tunability --- metamaterials --- multi-functionalities --- terahertz --- refraction index sensor --- n/a
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The book "Green Synthesis of Nanomaterials and Their Biological Applications" present novel advances about the green synthesis of nanomaterials, as well as on the possible biological applications, including studies dealing with entomology, parasitology, biomedicine, and environmental research.
metamaterial --- multiple resonances --- biochemical sensing --- environment sensor --- net formulations --- stored product insects --- knockdown --- mortality --- long-term effect --- European grapevine moth --- green pesticide --- insect pest --- Integrated Pest Management --- Larvicide --- nano-insecticide --- Tortricidae --- hydatid cyst --- protoscoleces --- nanomedicine --- in vitro --- in vivo --- ex vivo --- zinc oxide --- nanoparticles --- black tea extract --- gallic acid --- green synthesis --- antioxidants --- antimicrobial activity --- silver nanoparticles --- Diospyros malabarica --- antibacterial --- anticancer --- catalyst --- 4-nitrophenol --- electromechanically --- tunability --- metamaterials --- multi-functionalities --- terahertz --- refraction index sensor --- n/a
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The use of lipid-based nanosystems, including lipid nanoparticles (solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC)), nanoemulsions, and liposomes, among others, is widespread. Several researchers have described the advantages of different applications of these nanosystems. For instance, they can increase the targeting and bioavailability of drugs, improving therapeutic effects. Their use in the cosmetic field is also promising, owing to their moisturizing properties and ability to protect labile cosmetic actives. Thus, it is surprising that only a few lipid-based nanosystems have reached the market. This can be explained by the strict regulatory requirements of medicines and the occurrence of unexpected in vivo failure, which highlights the need to conduct more preclinical studies.Current research is focused on testing the in vitro, ex vivo, and in vivo efficacy of lipid-based nanosystems to predict their clinical performance. However, there is a lack of method validation, which compromises the comparison between different studies.This book brings together the latest research and reviews that report on in vitro, ex vivo, and in vivo preclinical studies using lipid-based nanosystems. Readers can find up-to-date information on the most common experiments performed to predict the clinical behavior of lipid-based nanosystems. A series of 15 research articles and a review are presented, with authors from 15 different countries, which demonstrates the universality of the investigations that have been carried out in this area.
Technology: general issues --- nanostructured lipid carriers (NLC) --- formulation optimization --- rivastigmine --- quality by design (QbD) --- nasal route --- nose-to-brain --- N-alkylisatin --- liposome --- urokinase plasminogen activator --- PAI-2 --- SerpinB2 --- breast cancer --- liposomes --- target delivery nanosystem --- FZD10 protein --- colon cancer therapy --- supersaturation --- silica-lipid hybrid --- spray drying --- lipolysis --- lipid-based formulation --- fenofibrate --- mesoporous silica --- oral drug delivery --- hyaluronic acid --- drug release --- light activation --- stability --- mobility --- biocorona --- dissolution enhancement --- phospholipids --- solid dosage forms --- porous microparticles --- nanoemulsion(s) --- phase-behavior --- DoE --- D-optimal design --- vegetable oils --- non-ionic surfactants --- efavirenz --- flaxseed oil --- nanostructured lipid carriers --- nanocarrier --- docohexaenoic acid --- neuroprotection --- neuroinflammation --- fluconazole --- Box‒Behnken design --- nanotransfersome --- ulcer index --- zone of inhibition --- rheological behavior --- ex vivo permeation --- nanomedicine --- cancer --- doxorubicin --- melanoma --- drug delivery --- ultrasound contrast agents --- phospholipid coating --- ligand distribution --- cholesterol --- acoustic response --- microbubble --- lipid phase --- dialysis --- ammonia --- intoxication --- cyanocobalamin --- vitamin B12 --- atopic dermatitis --- psoriasis --- transferosomes --- lipid vesicles --- skin topical delivery --- oligonucleotide --- self-emulsifying drug delivery systems --- hydrophobic ion pairing --- intestinal permeation enhancers --- Caco-2 monolayer --- clarithromycin --- solid lipid nanoparticles --- optimization --- permeation --- pharmacokinetics --- follicular targeting --- dexamethasone --- alopecia areata --- lipomers --- lipid polymer hybrid nanocapsules --- biodistribution --- skin --- ethyl cellulose --- nanostructured lipid carriers (NLC) --- formulation optimization --- rivastigmine --- quality by design (QbD) --- nasal route --- nose-to-brain --- N-alkylisatin --- liposome --- urokinase plasminogen activator --- PAI-2 --- SerpinB2 --- breast cancer --- liposomes --- target delivery nanosystem --- FZD10 protein --- colon cancer therapy --- supersaturation --- silica-lipid hybrid --- spray drying --- lipolysis --- lipid-based formulation --- fenofibrate --- mesoporous silica --- oral drug delivery --- hyaluronic acid --- drug release --- light activation --- stability --- mobility --- biocorona --- dissolution enhancement --- phospholipids --- solid dosage forms --- porous microparticles --- nanoemulsion(s) --- phase-behavior --- DoE --- D-optimal design --- vegetable oils --- non-ionic surfactants --- efavirenz --- flaxseed oil --- nanostructured lipid carriers --- nanocarrier --- docohexaenoic acid --- neuroprotection --- neuroinflammation --- fluconazole --- Box‒Behnken design --- nanotransfersome --- ulcer index --- zone of inhibition --- rheological behavior --- ex vivo permeation --- nanomedicine --- cancer --- doxorubicin --- melanoma --- drug delivery --- ultrasound contrast agents --- phospholipid coating --- ligand distribution --- cholesterol --- acoustic response --- microbubble --- lipid phase --- dialysis --- ammonia --- intoxication --- cyanocobalamin --- vitamin B12 --- atopic dermatitis --- psoriasis --- transferosomes --- lipid vesicles --- skin topical delivery --- oligonucleotide --- self-emulsifying drug delivery systems --- hydrophobic ion pairing --- intestinal permeation enhancers --- Caco-2 monolayer --- clarithromycin --- solid lipid nanoparticles --- optimization --- permeation --- pharmacokinetics --- follicular targeting --- dexamethasone --- alopecia areata --- lipomers --- lipid polymer hybrid nanocapsules --- biodistribution --- skin --- ethyl cellulose
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In the search for a healthier diet and facing the uncertainties about the consumption of artificial additives, the scientific community has explored in the last years the potential of natural compounds, such as polyphenols, carotenoids, chlorophylls, essential oils, tocopherols, vitamins, polysaccharides, among others, obtained from the most diverse sources, such as plants and fungi, for the replacement of the artificial additives in food, which has made these compounds recognized as “natural additives”. In addition to being considered less harmful to health, and giving a certain functional property to a foodstuff, natural additives can also provide a beneficial synergistic effect in the food matrix. For the consumers, diverse studies have been showed that regular ingestion of bioactive compounds, that can be applied as natural additives, can be helpful for the prevention/treatment of several chronic and aging illnesses, such as cardiovascular diseases and neurological disorders. However, there is much to be researched on the viable use of bioactive compounds and to overcome their chemical limitations so that it can actually be used as natural additives in foods. This book addresses, through 18 scientific articles, some natural compounds with potential for use as preservatives, nutritional additives, coloring agents, flavorings, and various agents.
Technology: general issues --- trisodium citrate --- microbial transglutaminase --- casein gels --- textural properties --- microstructures --- Urtica spp. --- bioactive compounds --- antioxidant activity --- antimicrobial activity --- traditional medicine --- food industry --- animal breeding --- beta cyclodextrin --- ewe’s milk --- cheese --- Manchego --- lipids --- cholesterol --- anthocyanin --- natural extract --- tetraethyl orthosilicate --- black carrot --- black rice flour --- anthocyanins --- low gluten muffins --- added value products --- soymilk --- enzyme modified soymilk --- skim milk --- rennet induced gelation --- rheological properties --- egg yolk --- cholesterol extraction --- granules extraction --- anionic chelating biopolymers --- ADME --- absorption --- chlorophylls --- chlorophyllin --- green colorant --- zinc-chlorophylls --- copper-chlorophyll --- coloring foodstuff --- natural colorants --- food colors --- pomelo peel --- essential oil --- anti-oxidant --- anti-melanogenic --- B16 melanoma cell --- oxygen-containing sulfur flavor molecules --- β-carotene --- bis(2-methyl-3-furyl) disulfide (BMFDS) --- oxidation products --- beetroot --- betalains --- semi-preparative RP-HPLC --- in vitro human gastrointestinal digestion --- ex vivo colon fermentation --- antioxidant ability --- malonildialdehyde --- extra virgin olive oil --- leaf addition --- Tunisian varieties --- phenolics --- tocopherols --- Prunus spinosa L. fruit epicarp --- wild fruit valorization --- cyanidin 3-rutinoside --- peonidin 3-rutinoside --- heat and ultrasound assisted extraction --- response surface methodology --- Ocimum basilicum var. purpurascens leaves --- red rubin basil --- Heat-Assisted Extraction --- extraction optimization --- polyphenols --- tiger nut --- by-products --- solvent extraction --- horchata de chufa --- triple TOF-LC-MS-MS --- Caenorhabditis elegans --- antioxidants --- bergenin --- stress resistance --- lifespan --- Huntington --- uxi --- Endopleura uchi --- Allium cepa red cv. --- Citrus limon --- pickling --- volatiles --- SPME --- chemometrics --- byproducts --- food additives --- antimicrobial --- antioxidant --- colorants --- texturizing agents --- foaming capacity and emulsifiers --- trisodium citrate --- microbial transglutaminase --- casein gels --- textural properties --- microstructures --- Urtica spp. --- bioactive compounds --- antioxidant activity --- antimicrobial activity --- traditional medicine --- food industry --- animal breeding --- beta cyclodextrin --- ewe’s milk --- cheese --- Manchego --- lipids --- cholesterol --- anthocyanin --- natural extract --- tetraethyl orthosilicate --- black carrot --- black rice flour --- anthocyanins --- low gluten muffins --- added value products --- soymilk --- enzyme modified soymilk --- skim milk --- rennet induced gelation --- rheological properties --- egg yolk --- cholesterol extraction --- granules extraction --- anionic chelating biopolymers --- ADME --- absorption --- chlorophylls --- chlorophyllin --- green colorant --- zinc-chlorophylls --- copper-chlorophyll --- coloring foodstuff --- natural colorants --- food colors --- pomelo peel --- essential oil --- anti-oxidant --- anti-melanogenic --- B16 melanoma cell --- oxygen-containing sulfur flavor molecules --- β-carotene --- bis(2-methyl-3-furyl) disulfide (BMFDS) --- oxidation products --- beetroot --- betalains --- semi-preparative RP-HPLC --- in vitro human gastrointestinal digestion --- ex vivo colon fermentation --- antioxidant ability --- malonildialdehyde --- extra virgin olive oil --- leaf addition --- Tunisian varieties --- phenolics --- tocopherols --- Prunus spinosa L. fruit epicarp --- wild fruit valorization --- cyanidin 3-rutinoside --- peonidin 3-rutinoside --- heat and ultrasound assisted extraction --- response surface methodology --- Ocimum basilicum var. purpurascens leaves --- red rubin basil --- Heat-Assisted Extraction --- extraction optimization --- polyphenols --- tiger nut --- by-products --- solvent extraction --- horchata de chufa --- triple TOF-LC-MS-MS --- Caenorhabditis elegans --- antioxidants --- bergenin --- stress resistance --- lifespan --- Huntington --- uxi --- Endopleura uchi --- Allium cepa red cv. --- Citrus limon --- pickling --- volatiles --- SPME --- chemometrics --- byproducts --- food additives --- antimicrobial --- antioxidant --- colorants --- texturizing agents --- foaming capacity and emulsifiers
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The analysis of circulating tumor cells (CTCs) as a real-time liquid biopsy approach can be used to obtain new insights into metastasis biology, and as companion diagnostics to improve the stratification of therapies and to obtain insights into the therapy-induced selection of cancer cells. In this book, we will cover all the different facets of CTCs to assemble a huge corpus of knowledge on cancer dissemination: technologies for their enrichment, detection, and characterization; their analysis at the single-cell level; their journey as CTC microemboli; their clinical relevance; their biology with the epithelial-to-mesenchymal transition (EMT); their stem-cell properties; their potential to initiate metastasis at distant sites; their ex vivo expansion; and their escape from the immune system.
n/a --- FOLFIRINOX --- immunofluorescence imaging --- AR-V7 --- circulating tumour cells --- chemoradioresistance --- CTC-based treatment decisions --- rVAR2 --- immunophenotyping --- immune system --- CellSearch® --- flow cytometry --- clinical trials --- circulating tumor DNA --- synaptophysin --- stem cells --- colorectal cancer --- melanoma --- CTC biology --- platelets --- AR --- CTC capture technology --- castration resistant prostate cancer (CRPC) --- PD-L1 expression --- rovalpituzumab tesirine --- HMB-45 --- thymidylate synthase --- ctDNA --- tumor cell dissemination --- solid cancers --- metastasis --- locally advanced rectal cancer --- miRNA --- epithelial-to-mesenchymal transition (EMT) --- NSCLC --- tumor biomarkers --- tumor stem cells --- circulating tumor cells --- major histocompatibility complex class I (MHCI) --- bone marrow --- heterogeneity --- cerebrospinal liquid biopsy --- fish --- glioma --- in vivo flow cytometry --- colorectal surgery --- CellSearch --- single-cell analysis --- disseminated tumor cells --- EasyCount slides --- microsatellite instability --- circulating plasma cells --- circulating leukemia cells --- ARV7 --- SLUG --- androgen receptor --- metastatic colorectal cancer --- leukocyte-derived extracellular vesicles --- prostate cancer (PCa) --- neutrophils --- liquid biopsy --- enzalutamide --- CD133 --- enrichment and detection technologies --- biomarkers --- immune checkpoint inhibitors --- biomarker --- RAD23B --- microbiome --- integrin B1 --- ACCEPT --- emboli --- small-cell lung carcinoma --- EPISPOT --- microfluidics --- early breast cancer --- circulating tumor cells (CTC) --- tumor-initiating cells (TICs) --- immunomodulation --- xenograft models --- CTC-derived xenografts --- malaria --- circulating tumor cells (CTCs) --- clinical utility --- exosomes --- liquid surgery --- ctRNA --- CTCs --- epithelial–mesenchymal transition --- targeted therapy --- hematological cells --- gene expression analysis --- hepatocellular carcinoma (HCC) --- breast cancer --- EpCAM enrichment --- prostate cancer --- CTC --- abiraterone --- fibronectin --- CTC-derived ex vivo models --- CTMat --- chromogranin A --- CTM --- exosome --- epithelial-mesenchymal transition
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Recently, microfluidic, nanofluidic and lab-on-a-chip devices have gained particular attention in biomedical applications. Due to their advantages, such as miniaturization, versatility, ease of use, cost-effectiveness, and the potential to replace animal models for drug development and testing, these devices hold tremendous potential to revolutionize the research of more effective treatments for several diseases that threaten human life. With integrated biosensors, these devices allow the development and design of micro- and nanoparticles to be studied in detail, modelling human physiology, investigating the molecular and cellular mechanisms underlying disease formation and progression, and gaining insights into the performance and long-term effects of responsive drug delivery nanocarriers. This Special Issue gathered research papers, and review articles focusing on novel microfluidic, nanofluidic and lab-on-a-chip devices for biomedical applications, addressing all steps related to fabrication, biosensor integration and development, characterization, numerical simulations and validation of the devices, optimization and, the translation of these devices from research labs to industry settings.
Medicine --- protein biomarker --- microarray --- microfluidic cassette --- multiplex measurement --- immunoassay --- point-of-care testing --- microfluidic device --- small intestine --- ex vivo --- histology --- embedded resin --- sectioning --- peptide biosensor --- lab-on-a-chip --- label-free detection --- peptide aptamers --- protein biomarkers --- microfluidic biochip --- troponin T --- computational simulations --- drug discovery --- organ-on-a-chip --- microfluidic devices --- preclinical models --- numerical simulations --- automation --- non-enzymatic --- DNA amplification --- L-DNA --- microfluidic --- fluorescence --- paper microfluidics --- sweat --- sensing --- hydrogels --- lactate --- osmotic pumping --- evaporation --- capillary --- wicking --- biochemical assay --- microfluidics --- cell trap --- RBC --- evolutionary algorithm --- generative design --- artificial intelligence --- organ-on-chip --- liver-on-chip --- liver disease --- multi-level microfluidic device --- live cell imaging --- long-term microscopy imaging --- focus drifting --- immersion oil viscosity --- bacterial population dynamics --- single-cell studies --- E. coli --- mother machine --- computational fluid dynamics --- cancer-on-chip --- xenograft --- colorectal cancer --- pharmacodynamics --- pharmacokinetics --- drug efficacy --- oxaliplatin --- microfabrication --- microphysiological system --- biophysical stimuli --- biochemical stimuli --- in vitro cell culture --- cortical neurons --- hippocampal neurons --- electrical stimulation --- Micro-Electrode Arrays --- engineered neuronal networks --- polydimethylsiloxane --- microchannels --- in vivo micro bioreactor --- additive manufacturing --- poly-(ethylene glycol)-diacrylate --- biocompatibility --- COVID-19 --- diagnosis --- image analysis --- PCR --- SARS-CoV-2 --- n/a
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