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Flow cytometry : select topics
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ISBN: 9535125516 9535154451 9535125508 Year: 2016 Publisher: IntechOpen

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Flow cytometry - Select Topics is a collection of chapters that illustrate the constantly evolving application of flow cytometry to diverse areas of research or clinical investigations. It includes chapters on the utilization of flow cytometry in the fields of human reproduction and fertility, platelet function, apoptosis, inflammation research, leukemia immunophenotyping, and transplantation.


Book
Flow cytometry
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ISBN: 1628087102 9781628087109 9781628087093 1628087099 Year: 2013 Publisher: New York

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Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characte


Book
Clinical flow cytometry
Authors: ---
ISBN: 1536146447 9781536146448 1536146439 9781536146431 Year: 2019 Publisher: New York

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Flow cytometry today : everything you need to know about flow cytometry
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ISBN: 9783031108365 Year: 2023 Publisher: Cham, Switzerland : Springer,


Book
Multidimensional flow cytometry techniques for novel highly informative assays
Authors: ---
ISBN: 1789233453 1789233445 1838813616 Year: 2018 Publisher: IntechOpen

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Flow cytometry's informative potential has been underestimated for many years because of a lack of adequate instruments, automation, reagents, and know-how to approach, integrate, and also substitute other techniques giving single information per assay. In the last decade, flow cytometers have become capable of performing high-throughput screening and high content analysis, evaluating tens of different samples' features in a single run up to 1536 formats on multiple cell populations. The introduction of imaging flow cytometry has filled the gap between flow cytometry and conventional high content imaging screening, putting flow cytometry at the center of many laboratories, which can now cover with a single instrument the vast majority of needs in research programs. The flow cytometry community is a multidisciplinary and diversified group with many different interests and fields of action. These characteristics have prompted the evolution of the techniques, applications, and instruments that allow the use of complex, sophisticated, and standardized and reliable flow cytometric assays in academic and industrial programs.

Flow cytometry for biotechnology
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ISBN: 0197561896 0190293039 1280428627 1423735021 0198034784 1602565694 9780198034780 9781423735021 9781602565692 9781280428623 9786610428625 661042862X 0195183142 0195152344 9780195152340 9780195183146 Year: 2005 Publisher: New York Oxford University Press

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Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity and high throughput informatics with applications in various fields.

Flow cytometry and cell sorting
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ISBN: 3540555943 0387555943 3662027879 3662027852 9783540555940 9780387555942 Year: 1993 Publisher: Berlin Springer

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Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced. &#13;&#13;In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.

Flow cytometry : principles and applications.
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ISBN: 1280972165 9786610972166 1597454516 1588296911 Year: 2007 Publisher: Totowa Humana press

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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

Flow cytometry in hematopathology : a visual approach to data analysis and interpretation
Authors: --- ---
ISBN: 1281339563 9786611339562 1597451622 1588298558 Year: 2007 Publisher: Totowa, N.J. : Humana Press,

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Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. In this second edition of this well received book, the authors have revised and expanded the text and added more than 100 figures to reflect the recent advances in the field. The focus of the additional material is on the TCR-Vb eight-tube kit which has greatly facilitated the evaluation mature T-cell disorders, and on the DNA dye DRAQ5 for improved grading of malignant lymphoma. The authors also include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis. Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening their FCM data analysis skills on a wide spectrum of hematologic disorders.

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