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Flow cytometry - Select Topics is a collection of chapters that illustrate the constantly evolving application of flow cytometry to diverse areas of research or clinical investigations. It includes chapters on the utilization of flow cytometry in the fields of human reproduction and fertility, platelet function, apoptosis, inflammation research, leukemia immunophenotyping, and transplantation.
Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Medical genetics
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Flow cytometry is a method to conduct a multiparameter analysis of cells suspended in liquid and passing through a laser beam. As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. In this book, the authors discuss the principles, methodology and applications of flow cytometry. Topics include flow cytometry and epifluorescence analyses of freshwater bacterio- and virioplankton communities; application of flow cytometry in phenotyping, analysis and functional characte
Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry
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Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry
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Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Citometria de fluxe
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Flow cytometry's informative potential has been underestimated for many years because of a lack of adequate instruments, automation, reagents, and know-how to approach, integrate, and also substitute other techniques giving single information per assay. In the last decade, flow cytometers have become capable of performing high-throughput screening and high content analysis, evaluating tens of different samples' features in a single run up to 1536 formats on multiple cell populations. The introduction of imaging flow cytometry has filled the gap between flow cytometry and conventional high content imaging screening, putting flow cytometry at the center of many laboratories, which can now cover with a single instrument the vast majority of needs in research programs. The flow cytometry community is a multidisciplinary and diversified group with many different interests and fields of action. These characteristics have prompted the evolution of the techniques, applications, and instruments that allow the use of complex, sophisticated, and standardized and reliable flow cytometric assays in academic and industrial programs.
Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Medicine --- Health Sciences --- Cell Biology
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Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity and high throughput informatics with applications in various fields.
Flow cytometry. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry
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Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced. In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies. In basic research, analysis and isolation of cells according to the correlated expression of up to 10 gene products, expressed within, on or around the cell have become possible.
DIF (directe immunofluorescentie) --- Histology. Cytology --- DNA (deoxyribonucleic acid) --- flowcytometrie --- immunologie --- Immunology. Immunopathology --- Flow cytometry. --- Flow cytometry --- moleculaire biologie --- IMMUNOFLUORESCENCE --- CELL SORTING --- Deoxypentose Nucleic Acid DNA --- Monograph --- Immunofluorescence. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Fluorescence --- Immunocytochemistry --- Immunoglobulins --- Immunology --- Technique
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Cytodiagnosis. --- Flow Cytometry. --- Cytofluorometry, Flow --- Cytometry, Flow --- Flow Microfluorimetry --- Fluorescence-Activated Cell Sorting --- Microfluorometry, Flow --- Cell Sorting, Fluorescence-Activated --- Cell Sortings, Fluorescence-Activated --- Cytofluorometries, Flow --- Cytometries, Flow --- Flow Cytofluorometries --- Flow Cytofluorometry --- Flow Cytometries --- Flow Microfluorometries --- Flow Microfluorometry --- Fluorescence Activated Cell Sorting --- Fluorescence-Activated Cell Sortings --- Microfluorimetry, Flow --- Microfluorometries, Flow --- Sorting, Fluorescence-Activated Cell --- Sortings, Fluorescence-Activated Cell --- Cytodiagnoses --- Cytological Techniques --- Periodicals --- Health Sciences --- Life Sciences --- Pathology --- Biology --- Cytology, Cell Biology --- Molecular biology --- Genetics --- moleculaire biologie --- Cytodiagnosis --- Flow Cytometry
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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Flow cytometry. --- Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Separation --- Cell separation --- Cytodiagnosis --- Flow cytometry --- Cell Separation --- Flow Cytometry --- Cytologic diagnosis --- Diagnosis, Cytologic --- Diagnostic cytopathology --- Diagnosis, Laboratory --- Pathology, Cellular --- methods
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Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly experienced in laboratory hematopathology and FCM offer a unique systematic approach to FCM data analysis and interpretation based on the visual inspection of dual parameter FCM graphics. This step-by-step approach to optimal FCM data analysis is demonstrated by means of numerous FCM graphics derived from actual well-documented clinical cases. In this second edition of this well received book, the authors have revised and expanded the text and added more than 100 figures to reflect the recent advances in the field. The focus of the additional material is on the TCR-Vb eight-tube kit which has greatly facilitated the evaluation mature T-cell disorders, and on the DNA dye DRAQ5 for improved grading of malignant lymphoma. The authors also include notes on "tricks of the trade" and pitfalls to avoid. The discussion, covering leukemias, lymphomas, and other conditions, moves from simple to complex specimens, with an emphasis on visual pattern analysis. Richly illustrated and highly instructive, Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation offers clinical pathologists, hematopathologists, and specialists in laboratory medicine a much-needed guide with a practical and logical approach for sharpening their FCM data analysis skills on a wide spectrum of hematologic disorders.
Blood --- Flow cytometry --- Hematopoietic stem cell disorders --- Immunophenotyping --- Pathophysiology. --- Methodology. --- Diagnosis. --- Lymphocyte immunophenotyping --- Lymphocyte subtyping --- Lymphocytes --- Hematopoietic stem cell diseases --- Hematopoietic stem cells --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Body fluids --- Fear of blood --- Classification --- Diseases
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