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Techniques for separating cells are needed in many areas of cell biology. This text presents modern methods from the laboratories of experts in the field and includes tested, reproducible protocols, hints and tips and troubleshooting techniques.
Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Separation
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Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a ba
Magnetic properties of solids --- Fysicochemical separation methods --- Histology. Cytology --- Cell separation. --- Magnetic separation. --- Separation (Technology) --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation --- Cell separation --- Magnetic separation
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This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.
Regenerative medicine. --- Stem cells. --- Regenerative Medicine and Tissue Engineering. --- Stem Cell Biology. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Medicine --- Regeneration (Biology) --- immune response --- cell sorting --- stem cells --- cell isolation --- apoptosis --- Open Access
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
single-cell sequencing --- Single-Cell isolation --- single-cell fluorescence in situ hybridization --- Single-cell multi-omics --- Single-cell microfluidics --- single-cell experimental design --- single-cell bioinformatics --- cell cycling and cell size --- Single-cell mass spectrometry --- Cell heterogeneity
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Science: general issues --- Biology, life sciences --- single-cell sequencing --- Single-Cell isolation --- single-cell fluorescence in situ hybridization --- Single-cell multi-omics --- Single-cell microfluidics --- single-cell experimental design --- single-cell bioinformatics --- cell cycling and cell size --- Single-cell mass spectrometry --- Cell heterogeneity --- single-cell sequencing --- Single-Cell isolation --- single-cell fluorescence in situ hybridization --- Single-cell multi-omics --- Single-cell microfluidics --- single-cell experimental design --- single-cell bioinformatics --- cell cycling and cell size --- Single-cell mass spectrometry --- Cell heterogeneity
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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Flow cytometry. --- Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Separation --- Cell separation --- Cytodiagnosis --- Flow cytometry --- Cell Separation --- Flow Cytometry --- Cytologic diagnosis --- Diagnosis, Cytologic --- Diagnostic cytopathology --- Diagnosis, Laboratory --- Pathology, Cellular --- methods
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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
Science: general issues --- Biology, life sciences --- single-cell sequencing --- Single-Cell isolation --- single-cell fluorescence in situ hybridization --- Single-cell multi-omics --- Single-cell microfluidics --- single-cell experimental design --- single-cell bioinformatics --- cell cycling and cell size --- Single-cell mass spectrometry --- Cell heterogeneity
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Biological techniques --- Immunology. Immunopathology --- Immunity, Cellular. --- Cell separation --- Cellular immunity --- -Cell-mediated immunity --- Cellular immunology --- Clonal selection theory --- Immune response --- Immunity --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Cellular Immune Response --- Cell-Mediated Immunity --- Cell Mediated Immunity --- Cell-Mediated Immunities --- Cellular Immune Responses --- Cellular Immunities --- Cellular Immunity --- Immune Response, Cellular --- Immune Responses, Cellular --- Immunities, Cell-Mediated --- Immunities, Cellular --- Immunity, Cell-Mediated --- Response, Cellular Immune --- Lymphokines --- T-Lymphocytes --- Interleukin-12 --- Technique --- Separation --- Cell separation. --- Technique. --- -Technique --- Immunity, Cellular --- Cell-mediated immunity
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Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments.A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for e
Biological techniques --- Histology. Cytology --- Cell separation --- Cellules --- Séparation --- Cell Separation --- methods --- 57.088 --- 576.3 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Cell separation. --- Molecular biology --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Technique. --- Separation --- Cell Separation - methods
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This is the first volume to focus exclusively on plant cell separation processes and to link improvements in our scientific understanding with methods that may allow us to manipulate cell separation and adhesion to the benefit of the agricultural and horticultural industries. It will therefore be of interest to the experimental scientist and to those who wish to apply these techniques commercially.
Cell separation --- Cell adhesion --- Cell biology --- Cellules --- Séparation --- Adhésivité --- 576.52 --- 581.17 --- Phenomena at cell surfaces. Cell adhesion. Cell contacts --- Physiology of the cell --- Cell adhesion. --- Cell separation. --- Plant cell differentiation. --- Plant cellular control mechanisms. --- Plantes --- Adhésivité. --- Séparation. --- Cellules et tissus --- Différenciation. --- Régulation cellulaire. --- 581.17 Physiology of the cell --- 576.52 Phenomena at cell surfaces. Cell adhesion. Cell contacts --- Cytology. --- Séparation --- Adhésivité --- Plant cell differentiation --- Plant cellular control mechanisms --- Cellular control mechanisms --- Cell differentiation --- Plant cells and tissues --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Adherence, Cell --- Cell adherence --- Adhesion --- Cell interaction --- Membrane fusion --- Junctional complexes (Epithelium) --- Separation
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