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In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.

Molecular biology --- Vertebrates --- Xenopus --- Zebra danio --- Technique. --- Embryology --- Embryos. --- Vertebrata --- Brachydanio rerio --- Danio, Indian --- Danio, Zebra --- Danio rerio --- Indian danio --- Indian zebra fish --- Zebra fish, Indian --- Zebrafish (Zebra danio) --- African clawed frog --- Dactylethra --- Silurana --- Chordata --- Brachydanio --- Pipidae --- Developmental biology. --- Developmental Biology. --- Development (Biology) --- Biology --- Growth --- Ontogeny

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The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.

Zebra danio. --- Optogenetics. --- Genetic engineering --- Optical engineering --- Brachydanio rerio --- Danio, Indian --- Danio, Zebra --- Danio rerio --- Indian danio --- Indian zebra fish --- Zebra fish, Indian --- Zebrafish (Zebra danio) --- Brachydanio --- Neurobiology. --- Optics, Lasers, Photonics, Optical Devices. --- Biological and Medical Physics, Biophysics. --- Neurosciences --- Barilius rerio --- Brachydanio frankei --- Cyprinus chapalio --- Cyprinus rerio --- Danio frankei --- Danio lineatus --- Leopard danio --- Nuria rerio --- Perilampus striatus --- Rerio --- Striped danio --- Zebra fish (Danio) --- Danio --- Lasers. --- Photonics. --- Biophysics. --- Biological physics. --- Biological physics --- Biology --- Medical sciences --- Physics --- New optics --- Optics --- Light amplification by stimulated emission of radiation --- Masers, Optical --- Optical masers --- Light amplifiers --- Light sources --- Optoelectronic devices --- Nonlinear optics --- Optical parametric oscillators