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Protein blotting techniques have become common laboratory procedures in the past few years. This text is written by scientists with expertise in these techniques. The versatility of the methods utilizing these procedures have brought about the development of different solid support matrices, as well as a wide variety of protein detection methods. While the most commonly used method in protein blotting is the use of antibodies to detect protein antigens, this technology has been expanded to examine a number of different interactions between proteins and other proteins, as well as other molecules such as carbohydrates and DNA. These methods have further been adapted for amino acid sequencing and purification of proteins for use as immunogens. This book outlines, in detail, numerous protocols and procedures which should help investigators design methods which will be optimal for their specific use.

Western immunoblotting --- Blotting, Western. --- Proteins --- 577.112 --- 57.088.1 --- 57.088.1 Methods for analysis and estimation --- Methods for analysis and estimation --- 577.112 Proteins --- Blot, Western --- Immunoblot, Western --- Western Blot --- Western Immunoblot --- Immunoblotting, Western --- Western Blotting --- Western Immunoblotting --- Blots, Western --- Blottings, Western --- Immunoblots, Western --- Immunoblottings, Western --- Western Blots --- Western Blottings --- Western Immunoblots --- Western Immunoblottings --- Protein blotting --- Western blot analysis --- Western blotting --- Immunoblotting --- Laboratory manuals. --- analysis. --- Analysis --- Blotting, Western --- Laboratory manuals --- analysis --- Analysis. --- Proteins - analysis --- Western immunoblotting - Laboratory manuals