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Viral load --- Polymerase chain reaction --- Serologic tests --- Human --- Methods --- Viral load --- Polymerase chain reaction --- Serologic tests --- Human --- Methods
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Microfluidics and lab-on-a-chip have, in recent years, come to the forefront in diagnostics and detection. At point-of-care, in the emergency room, and at the hospital bed or GP clinic, lab-on-a-chip offers the potential to rapidly detect time-critical and life-threatening diseases such as sepsis and bacterial meningitis. Furthermore, portable and user-friendly diagnostic platforms can enable disease diagnostics and detection in resource-poor settings where centralised laboratory facilities may not be available. At point-of-use, microfluidics and lab-on-chip can be applied in the field to rapidly identify plant pathogens, thus reducing the need for damaging broad spectrum pesticides while also reducing food losses. Microfluidics can also be applied to the continuous monitoring of water quality and can support policy-makers and protection agencies in protecting the environment. Perhaps most excitingly, microfluidics also offers the potential to enable entirely new diagnostic tests that cannot be implemented using conventional laboratory tools. Examples of microfluidics at the frontier of new medical diagnostic tests include early detection of cancers through circulating tumour cells (CTCs) and highly sensitive genetic tests using droplet-based digital PCR.This Special Issue on “Advances in Microfluidics Technology for Diagnostics and Detection” aims to gather outstanding research and to carry out comprehensive coverage of all aspects related to microfluidics in diagnostics and detection.
biosensors --- LoaD platforms --- microfluidics --- centrifugal microfluidics --- PoC devices --- SARS-CoV-2 --- COVID-19 --- nano-qPCR --- ultra-sensitive --- viral RNA --- viral load --- detection --- LabDisk --- vector-borne diseases --- malaria --- arboviruses --- insecticide resistances --- mosquito monitoring --- SAW --- Pirani --- compact --- wireless --- vacuum --- sensing --- digital droplet polymerase chain reaction (ddPCR) --- multiplexing --- centrifugal step emulsification --- droplet stability --- droplet fluorescence evaluation --- nanoparticle --- lipoplex --- polyplex --- raspberry pi --- siRNA --- python --- n/a
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Microfluidics and lab-on-a-chip have, in recent years, come to the forefront in diagnostics and detection. At point-of-care, in the emergency room, and at the hospital bed or GP clinic, lab-on-a-chip offers the potential to rapidly detect time-critical and life-threatening diseases such as sepsis and bacterial meningitis. Furthermore, portable and user-friendly diagnostic platforms can enable disease diagnostics and detection in resource-poor settings where centralised laboratory facilities may not be available. At point-of-use, microfluidics and lab-on-chip can be applied in the field to rapidly identify plant pathogens, thus reducing the need for damaging broad spectrum pesticides while also reducing food losses. Microfluidics can also be applied to the continuous monitoring of water quality and can support policy-makers and protection agencies in protecting the environment. Perhaps most excitingly, microfluidics also offers the potential to enable entirely new diagnostic tests that cannot be implemented using conventional laboratory tools. Examples of microfluidics at the frontier of new medical diagnostic tests include early detection of cancers through circulating tumour cells (CTCs) and highly sensitive genetic tests using droplet-based digital PCR.This Special Issue on “Advances in Microfluidics Technology for Diagnostics and Detection” aims to gather outstanding research and to carry out comprehensive coverage of all aspects related to microfluidics in diagnostics and detection.
Medicine --- biosensors --- LoaD platforms --- microfluidics --- centrifugal microfluidics --- PoC devices --- SARS-CoV-2 --- COVID-19 --- nano-qPCR --- ultra-sensitive --- viral RNA --- viral load --- detection --- LabDisk --- vector-borne diseases --- malaria --- arboviruses --- insecticide resistances --- mosquito monitoring --- SAW --- Pirani --- compact --- wireless --- vacuum --- sensing --- digital droplet polymerase chain reaction (ddPCR) --- multiplexing --- centrifugal step emulsification --- droplet stability --- droplet fluorescence evaluation --- nanoparticle --- lipoplex --- polyplex --- raspberry pi --- siRNA --- python --- biosensors --- LoaD platforms --- microfluidics --- centrifugal microfluidics --- PoC devices --- SARS-CoV-2 --- COVID-19 --- nano-qPCR --- ultra-sensitive --- viral RNA --- viral load --- detection --- LabDisk --- vector-borne diseases --- malaria --- arboviruses --- insecticide resistances --- mosquito monitoring --- SAW --- Pirani --- compact --- wireless --- vacuum --- sensing --- digital droplet polymerase chain reaction (ddPCR) --- multiplexing --- centrifugal step emulsification --- droplet stability --- droplet fluorescence evaluation --- nanoparticle --- lipoplex --- polyplex --- raspberry pi --- siRNA --- python
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Plant viruses cause many of the most important diseases threatening crops worldwide. Over the last quarter of a century, an increasing number of plant viruses have emerged in various parts of the world, especially in the tropics and subtropics. As is generally observed for plant viruses, most of the emerging viruses are transmitted horizontally by biological vectors, mainly insects. Reverse genetics using infectious clones—available for many plant viruses—has been used for identification of viral determinants involved in virus–host and virus–vector interactions. Although many studies have identified a number of factors involved in disease development and transmission, the precise mechanisms are unknown for most of the virus–plant–vector combinations. In most cases, the diverse outcomes resulting from virus–virus interactions are poorly understood. Although significant advances have been made towards understand the mechanisms involved in plant resistance to viruses, we are far from being able to apply this knowledge to protect cultivated plants from the all viral threats.The aim of this Special Issue was to provide a platform for researchers interested in plant virology to share their recent results. To achieve this, we invited the plant virology community to submit research articles, short communications and reviews related to the various aspects of plant virology: ecology, virus–plant host interactions, virus–vector interactions, virus–virus interactions, and control strategies. This issue contains some of the best current research in plant virology.
whitefly --- begomovirus --- Vta1 --- virus transmission --- coat proteins --- membrane association --- topology --- cilevirus --- movement protein --- p29 capsid protein --- barley yellow dwarf virus --- BYDV --- wheat --- barley --- yield loss --- vectors --- aphids --- persistent virus --- Amalgaviridae --- synergism --- antagonism --- vsiRNAs --- miRNAs --- mixed-infections --- Arabidopsis thaliana --- Cucumber mosaic virus --- genome-wide association studies --- plant–virus interaction --- seed transmission --- virulence --- callose --- coat protein --- plasmodesmata --- triple gene block --- viral suppressor --- virus movement --- virus replication complex --- TYLCD --- TYLCV --- tomato --- Solanum lycopersicum --- disease resistance --- plant breeding --- PAMP-triggered immunity --- effector-triggered immunity --- RNA silencing --- viral suppressors --- NIK1 --- PTI --- ETI --- geminiviruses --- host jumping --- viral evolution --- trade-off --- plant virus --- RNA virus --- potyvirus --- Plum pox virus --- VPg --- eIF4E --- high-throughput sequencing --- bioinformatics --- detection --- discovery --- MinION --- nanopore sequencing --- rolling circle amplification --- viral metagenomics --- CRESS DNA --- capulavirus --- homopolymer --- Begomovirus --- cucumber --- mechanical inoculation --- real-time PCR --- viral load --- QTLs --- resistance --- Geminiviridae --- sweepoviruses --- DNA satellites --- Deltasatellite --- helper virus range --- transreplication --- high-throughput sequencing (HTS) --- virus --- dsRNA --- total RNA --- OLV1 --- LRNV --- ToFBV --- ASGV --- host adaptation --- virus evolution --- n/a --- plant-virus interaction
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Plant viruses cause many of the most important diseases threatening crops worldwide. Over the last quarter of a century, an increasing number of plant viruses have emerged in various parts of the world, especially in the tropics and subtropics. As is generally observed for plant viruses, most of the emerging viruses are transmitted horizontally by biological vectors, mainly insects. Reverse genetics using infectious clones—available for many plant viruses—has been used for identification of viral determinants involved in virus–host and virus–vector interactions. Although many studies have identified a number of factors involved in disease development and transmission, the precise mechanisms are unknown for most of the virus–plant–vector combinations. In most cases, the diverse outcomes resulting from virus–virus interactions are poorly understood. Although significant advances have been made towards understand the mechanisms involved in plant resistance to viruses, we are far from being able to apply this knowledge to protect cultivated plants from the all viral threats.The aim of this Special Issue was to provide a platform for researchers interested in plant virology to share their recent results. To achieve this, we invited the plant virology community to submit research articles, short communications and reviews related to the various aspects of plant virology: ecology, virus–plant host interactions, virus–vector interactions, virus–virus interactions, and control strategies. This issue contains some of the best current research in plant virology.
Research & information: general --- Biology, life sciences --- whitefly --- begomovirus --- Vta1 --- virus transmission --- coat proteins --- membrane association --- topology --- cilevirus --- movement protein --- p29 capsid protein --- barley yellow dwarf virus --- BYDV --- wheat --- barley --- yield loss --- vectors --- aphids --- persistent virus --- Amalgaviridae --- synergism --- antagonism --- vsiRNAs --- miRNAs --- mixed-infections --- Arabidopsis thaliana --- Cucumber mosaic virus --- genome-wide association studies --- plant-virus interaction --- seed transmission --- virulence --- callose --- coat protein --- plasmodesmata --- triple gene block --- viral suppressor --- virus movement --- virus replication complex --- TYLCD --- TYLCV --- tomato --- Solanum lycopersicum --- disease resistance --- plant breeding --- PAMP-triggered immunity --- effector-triggered immunity --- RNA silencing --- viral suppressors --- NIK1 --- PTI --- ETI --- geminiviruses --- host jumping --- viral evolution --- trade-off --- plant virus --- RNA virus --- potyvirus --- Plum pox virus --- VPg --- eIF4E --- high-throughput sequencing --- bioinformatics --- detection --- discovery --- MinION --- nanopore sequencing --- rolling circle amplification --- viral metagenomics --- CRESS DNA --- capulavirus --- homopolymer --- Begomovirus --- cucumber --- mechanical inoculation --- real-time PCR --- viral load --- QTLs --- resistance --- Geminiviridae --- sweepoviruses --- DNA satellites --- Deltasatellite --- helper virus range --- transreplication --- high-throughput sequencing (HTS) --- virus --- dsRNA --- total RNA --- OLV1 --- LRNV --- ToFBV --- ASGV --- host adaptation --- virus evolution --- whitefly --- begomovirus --- Vta1 --- virus transmission --- coat proteins --- membrane association --- topology --- cilevirus --- movement protein --- p29 capsid protein --- barley yellow dwarf virus --- BYDV --- wheat --- barley --- yield loss --- vectors --- aphids --- persistent virus --- Amalgaviridae --- synergism --- antagonism --- vsiRNAs --- miRNAs --- mixed-infections --- Arabidopsis thaliana --- Cucumber mosaic virus --- genome-wide association studies --- plant-virus interaction --- seed transmission --- virulence --- callose --- coat protein --- plasmodesmata --- triple gene block --- viral suppressor --- virus movement --- virus replication complex --- TYLCD --- TYLCV --- tomato --- Solanum lycopersicum --- disease resistance --- plant breeding --- PAMP-triggered immunity --- effector-triggered immunity --- RNA silencing --- viral suppressors --- NIK1 --- PTI --- ETI --- geminiviruses --- host jumping --- viral evolution --- trade-off --- plant virus --- RNA virus --- potyvirus --- Plum pox virus --- VPg --- eIF4E --- high-throughput sequencing --- bioinformatics --- detection --- discovery --- MinION --- nanopore sequencing --- rolling circle amplification --- viral metagenomics --- CRESS DNA --- capulavirus --- homopolymer --- Begomovirus --- cucumber --- mechanical inoculation --- real-time PCR --- viral load --- QTLs --- resistance --- Geminiviridae --- sweepoviruses --- DNA satellites --- Deltasatellite --- helper virus range --- transreplication --- high-throughput sequencing (HTS) --- virus --- dsRNA --- total RNA --- OLV1 --- LRNV --- ToFBV --- ASGV --- host adaptation --- virus evolution
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Ranaviruses and other viruses within the family Iridoviridae, infect a wide range of ecologically and commercially important ectothermic vertebrates, i.e., bony fish, amphibians, and reptiles, and invertebrates, including agricultural and medical pests and cultured shrimp and crayfish, and are responsible for considerable morbidity and mortality. Understanding the impact of these various agents on diverse host species requires the combined efforts of ecologists, veterinarians, pathologists, comparative immunologists and molecular virologists. Unfortunately, investigators involved in these studies often work in discipline-specific silos that preclude interaction with others whose insights and approaches are required to comprehensively address problems related to ranavirus/iridovirus disease. Our intent here is to breakdown these silos and provide a forum where diverse researchers with a common interest in ranavirus/iridovirus biology can profitably interact. As a colleague once quipped, “Three people make a genius.” We are hoping to do something along those lines by presenting a collection of research articles dealing with issues of anti-viral immunity, identification of a potentially novel viral genus exemplified by erythrocytic necrosis virus, viral inhibition of innate immunity, identification of novel hosts for lymphocystivirus and invertebrate iridoviruses, and modelling studies of ranavirus transmission. Collectively these and others will exemplify the breadth of ongoing studies focused on this virus family.
risk assessment --- n/a --- CQIV --- mathematical models --- amphibian --- iridovirus --- ISDL --- Exopalaemon carinicauda --- viral load --- virus isolation --- European chub --- outbreak --- Unconventional T cell --- early detection --- susceptible species --- viral immune evasion --- DNA virus --- Rana grylio virus --- antibody --- intracellular localization --- Rana grylio virus (RGV) --- British Columbia --- Iridoviridae --- Andrias davidianus ranavirus --- viral infection --- susceptible-infected (SI) models --- yeast two-hybrid (Y2H) --- prevalence --- host-pathogen interactions --- Pacific herring --- Procambarus clarkii --- Bayesian inference --- eDNA --- amphibians --- Artemia spp. --- ranavirosis --- cross-species transmission --- FV3 --- SHIV --- Gryllus bimaculatus --- Pacific salmon --- NF-?B --- cricket --- IIV-6 --- virus binding --- erythrocytic necrosis virus (ENV) --- envelope protein --- iridovirus core proteins --- emerging infection --- host --- Ranavirus --- white head --- Rana temporaria --- Imd --- biosecurity --- antiviral immunity --- Decapodiridovirus --- endemic disease --- Macrobrachium rosenbergii --- co-immunoprecipitation (Co-IP) --- Common frog --- aquatic animals --- virus surveillance --- immunomodulators --- frog virus 3 --- ELISA --- DIV1 --- megalocytivirus --- Lymphocystis disease virus --- bearded dragon --- susceptibility --- protein interaction --- Pogona vitticeps --- viral erythrocytic necrosis (VEN) --- histopathology --- epidemiology --- native-fish conservation --- viral transmission --- Sparus aurata --- immunohistochemistry --- lizard --- disease dynamics --- immunofluorescence --- transmission modelling --- Macrobrachium nipponense --- interferon --- nonclassical MHC --- heparan sulfate --- ranavirus --- Mexico
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