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The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Medical microbiology, virology, parasitology --- Molecular biology --- Gene therapy --- Genetic vectors --- Transfection --- Viral genetics --- Virus-vector relationships --- Organisms --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Viruses --- Genetic Vectors --- Medicine --- Health & Biological Sciences --- Pathology --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Animal Viruses --- Zoophaginae --- Animal Virus --- Virus --- Virus, Animal --- Viruses, Animal --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Vector-virus relationships --- Virus genetics --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Therapy, Gene --- Genetics --- Human genetics. --- Medical virology. --- Gene therapy. --- Human Genetics. --- Virology. --- Gene Therapy. --- Medical microbiology --- Virology --- Virus diseases --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology

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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Gene therapy --- Gene Transfer Techniques --- Genetic Vectors --- Genetic Techniques --- Genetic Structures --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Pathology --- Medicine --- Health & Biological Sciences --- Methodology --- Genetic Therapy --- Thérapie génique --- methods. --- Methodology. --- Méthodologie --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Therapy, Gene --- methods --- Human genetics. --- Animal genetics. --- Gene therapy. --- Human Genetics. --- Animal Genetics and Genomics. --- Gene Therapy. --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology