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Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.

Sirtuins. --- Proteins. --- Proteins --- Group III Histone Deacetylases --- Intracellular Signaling Peptides and Proteins --- ADP Ribose Transferases --- Histone Deacetylases --- Pentosyltransferases --- Amino Acids, Peptides, and Proteins --- Glycosyltransferases --- Amidohydrolases --- Transferases --- Chemicals and Drugs --- Hydrolases --- Enzymes --- Enzymes and Coenzymes --- Sirtuins --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Silent Mating Type Information Regulator 2-like Proteins --- Sir2-like Proteins --- Silent Mating Type Information Regulator 2 like Proteins --- Sir2 like Proteins --- Coenzymes and Enzymes --- Biocatalysts --- Transferase --- Glycoside Transferases --- Transferases, Glycoside --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Class I Histone Deacetylases --- Class II Histone Deacetylases --- HDAC Proteins --- Histone Deacetylase --- Histone Deacetylase Complexes --- Complexes, Histone Deacetylase --- Deacetylase Complexes, Histone --- Deacetylase, Histone --- Deacetylases, Histone --- ADP Ribose Transferase --- ADPRT --- ADPRTs --- ART Transferase --- ART Transferases --- ARTase --- ARTases --- Mono ADP-ribose Transferases --- Mono ADPribose Transferase --- Mono ADPribose Transferases --- Mono(ADP-Ribose) Transferase --- Mono(ADP-Ribosyl)transferase --- Mono(ADPribosyl)transferase --- Mono-ADP-Ribosyltransferase --- MonoADPribosyltransferase --- NAD ADP-Ribosyltransferase --- NAD(+)-L-arginine ADP-D-ribosyltransferase --- NAD-Agmatine ADP-Ribosyltransferase --- NAD-Arginine ADP-Ribosyltransferase --- NADP-ADPRTase --- NADP-Arginine ADP-Ribosyltransferase --- ADP-Ribosyltransferase --- Mono(ADP-Ribose) Transferases --- NAD(P)(+)-Arginine ADP-Ribosyltransferase --- NAD+ ADP-Ribosyltransferase --- ADP Ribosyltransferase --- ADP-Ribosyltransferase, NAD --- ADP-Ribosyltransferase, NAD+ --- ADP-Ribosyltransferase, NAD-Agmatine --- ADP-Ribosyltransferase, NAD-Arginine --- ADP-Ribosyltransferase, NADP-Arginine --- ADP-ribose Transferases, Mono --- ADPribose Transferase, Mono --- ADPribose Transferases, Mono --- Mono ADP Ribosyltransferase --- Mono ADP ribose Transferases --- NAD ADP Ribosyltransferase --- NAD Agmatine ADP Ribosyltransferase --- NAD Arginine ADP Ribosyltransferase --- NAD+ ADP Ribosyltransferase --- NADP ADPRTase --- NADP Arginine ADP Ribosyltransferase --- Ribose Transferase, ADP --- Ribose Transferases, ADP --- Transferase, ADP Ribose --- Transferase, ART --- Transferase, Mono ADPribose --- Transferases, ADP Ribose --- Transferases, ART --- Transferases, Mono ADP-ribose --- Transferases, Mono ADPribose --- Intracellular Signaling Peptides --- Intracellular Signaling Proteins --- Peptides, Intracellular Signaling --- Proteins, Intracellular Signaling --- Signaling Peptides, Intracellular --- Signaling Proteins, Intracellular --- Proteids --- Amidases --- NAD-Dependent Histone Deacetylases --- Sir2-like Deacetylases --- Sirtuin Histone Deacetylases --- Deacetylases, NAD-Dependent Histone --- Deacetylases, Sir2-like --- Histone Deacetylases, NAD-Dependent --- NAD Dependent Histone Deacetylases --- Sir2 like Deacetylases --- Life sciences. --- Proteomics. --- Animal genetics. --- Life Sciences. --- Animal Genetics and Genomics. --- Genetics --- Molecular biology --- Biosciences --- Sciences, Life --- Science