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Breast Neoplasms --- Immunohistochemistry --- Proto-Oncogene Proteins c-bcl-2 --- Tissue Fixation --- Glutathione --- metabolism --- methods

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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.Key Features* Authored by the key leaders in the biological electron microscopy field* Illustrates both optimal

Cytodiagnosis --- Microscopy, Electron --- Electronics, Medical --- Tissue Fixation --- Electron microscopy --- Medical electronics. --- Microscopie électronique --- Electronique en médecine --- methods --- Technique. --- Technique --- Medical microscopy --- Microscopie électronique --- Electronique en médecine --- ELSEVIER-B EPUB-LIV-FT --- Microscopy, Medical --- Diagnosis, Laboratory --- Microscopy --- Electron microscopes

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Biological techniques --- Histology. Cytology --- Histocytochemistry. --- Histological Techniques. --- Tissue Preservation. --- Tissues --- -Histochemistry --- -Biochemistry --- Histology --- Organs (Anatomy) --- Preservation, Tissue --- Preservations, Tissue --- Tissue Preservations --- Tissue Transplantation --- Tissue and Organ Harvesting --- Histologic Technic --- Histologic Technics --- Histologic Technique --- Histologic Techniques --- Histological Technics --- Technic, Histologic --- Technics, Histologic --- Technique, Histologic --- Techniques, Histologic --- Histological Technic --- Histological Technique --- Technic, Histological --- Technics, Histological --- Technique, Histological --- Techniques, Histological --- Cytochemistry --- Preservation --- -Congresses --- Congresses --- methods --- Fixation (Histology) --- Histochemistry --- Congresses. --- -Preservation --- Histocytochemistry --- Histological Techniques --- Tissue Preservation --- Tissue fixation --- Cytology --- Preservation&delete& --- Fixation --- Technique

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A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.   This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Biochemistry. --- Biomedical engineering -- Moral and ethical aspects. --- Human body -- Moral and ethical aspects. --- Pathology. --- Pathology, Molecular --- Clinical Laboratory Techniques --- Genetic Techniques --- Complex Mixtures --- Tissue Preservation --- Chemicals and Drugs --- Investigative Techniques --- Histocytological Preparation Techniques --- Histological Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cytological Techniques --- Sequence Analysis --- Molecular Diagnostic Techniques --- Tissue Extracts --- Tissue Fixation --- Medicine --- Health & Biological Sciences --- Pathology --- Pharmacy, Therapeutics, & Pharmacology --- Preservation of organs, tissues, etc. --- Tissue banks. --- Banks, Organ --- Banks, Tissue --- Banks, Transplant --- Organ banks --- Transplant banks --- Organ preservation (Anatomy) --- Organs (Anatomy) --- Preservation --- Medicine. --- Molecular biology. --- Oncology. --- Medicine & Public Health. --- Molecular Medicine. --- Biobanks --- Health facilities --- Procurement of organs, tissues, etc. --- Tissue banks --- Transplantation of organs, tissues, etc. --- Oncology  . --- Tumors --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Physicians --- Disease (Pathology) --- Diseases --- Medicine, Preventive --- Health Workforce --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology