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Would you change your genes if you could? As we confront the 'industrial revolution of the genome', the recent discoveries of Crispr-Cas9 technologies are offering, for the first time, cheap and effective methods for editing the human genome. This opens up startling new opportunities as well as significant ethical uncertainty. Tracing events across a fifty-year period, from the first gene splicing techniques to the present day, this is the story of gene editing - the science, the impact and the potential. Kozubek weaves together the fascinating stories of many of the scientists involved in the development of gene editing technology. Along the way, he demystifies how the technology really works and provides vivid and thought-provoking reflections on the continuing ethical debate. Ultimately, Kozubek places the debate in its historical and scientific context to consider both what drives scientific discovery and the implications of the 'commodification' of life.

Gene targeting. --- Genomes. --- Genetics --- Genomics --- Haploidy --- Targeting, Gene --- Genetic engineering

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Would you change your genes if you could? As we confront the 'industrial revolution of the genome', the recent discoveries of Crispr-Cas9 technologies are offering, for the first time, cheap and effective methods for editing the human genome. This opens up startling new opportunities as well as significant ethical uncertainty. Tracing events across a fifty-year period, from the first gene splicing techniques to the present day, this is the story of gene editing - the science, the impact and the potential. Kozubek weaves together the fascinating stories of many of the scientists involved in the development of gene editing technology. Along the way, he demystifies how the technology really works and provides vivid and thought-provoking reflections on the continuing ethical debate. Ultimately, Kozubek places the debate in its historical and scientific context to consider both what drives scientific discovery and the implications of the 'commodification' of life.

Gene targeting. --- Genomes. --- CRISPR-associated protein 9. --- Cas9 (CRISPR-associated protein 9) --- CRISPR/Cas9 --- Endonucleases --- Gene editing --- Genetics --- Genomics --- Haploidy --- Targeting, Gene --- Genetic engineering

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In Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting- one using a fusion protein to deliver a vector to a virus for possible therapeutic effect, and one entailing an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may revolutionize the entire field. Innovative and wide ranging, Gene Targeting Protocols offers today's researchers robust and proven gene-targeting techniques that are essential to understanding and regulating biological processes at the genetic level.

Electronic books. -- local. --- Gene expression. --- Gene targeting -- Laboratory manuals. --- Gene targeting --- Laboratory Manuals --- Gene Targeting --- Genetic Techniques --- Publication Formats --- Investigative Techniques --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genetics --- Biology --- Health & Biological Sciences --- Targeting, Gene --- Genetic engineering --- Genes --- Genetic regulation --- Expression --- Human genetics. --- Human Genetics. --- Heredity, Human --- Human biology --- Physical anthropology

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Gene targeting --- Gènes --- Laboratory manuals. --- Ciblage --- Manuels de laboratoire --- Laboratory manuals --- 57.088.6 --- 575.224 --- 575.113 --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- Mutations. Mutagenesis. Mutagens. --- Gene. Genetic apparatus. Genome --- 575.113 Gene. Genetic apparatus. Genome --- 575.224 Mutations. Mutagenesis. Mutagens. --- 57.088.6 Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques. --- Gènes --- Targeting, Gene --- Genetic engineering --- Methods and techniques for studying metabolism and biotransformation.Radioisotopes methods and techniques --- Mutations. Mutagenesis. Mutagens --- Gene targeting - Laboratory manuals

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The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.

Gene Transfer Techniques. --- Genetic Engineering. --- Stem Cells. --- Transgenes. --- Transgenes --- Genetic Techniques --- Genes --- Biological Science Disciplines --- Investigative Techniques --- Natural Science Disciplines --- Genome Components --- Disciplines and Occupations --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genome --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Physiology --- Gene Transfer Techniques --- Biology --- Health & Biological Sciences --- Genetics --- Biomedical Engineering --- Transgenic animals. --- Gene targeting --- Targeting, Gene --- Chemistry. --- Gene expression. --- Gene therapy. --- Medical genetics. --- Genetic engineering. --- Gene Therapy. --- Gene Function. --- Gene Expression. --- Genetic engineering --- Animals --- Transgenic organisms --- Animal genetic engineering --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Therapy, Gene --- Therapeutics --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Genetic regulation --- Genetic aspects --- Expression