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Biochemical Phenomena --- Genetic Processes --- Genetic Structures --- Molecular biology --- Biochemical Phenomena. --- Genetic Phenomena. --- Genetic Structures. --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetics --- Molecular Biology --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Biochemical Concepts --- Biochemical Phenomenon --- Biochemical Process --- Phenomena, Biochemical --- Biochemical Processes --- Biochemical Concept --- Concept, Biochemical --- Concepts, Biochemical --- Phenomenon, Biochemical --- Process, Biochemical --- Processes, Biochemical --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Biologie moléculaire
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Genetic Processes. --- Genetic Structures. --- Genetic Techniques. --- Molecular Biology --- 577.2 --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Phenomena --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- methods. --- Molecular bases of life. Molecular biology --- methods --- 577.2 Molecular bases of life. Molecular biology --- PXL-Tech 2018 --- moleculaire biologie --- genen --- Genetic Structures --- Genetic Techniques
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The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.
Antisense DNA --- Publication Formats --- Genetic Structures --- Genetic Phenomena --- Publication Characteristics --- Phenomena and Processes --- Laboratory Manuals --- Gene Library --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- DNA, Complementary --- Antisense nucleic acids --- Acides nucléiques antisens --- DNA Library --- cDNA Library --- DNA Libraries --- Gene Libraries --- Libraries, DNA --- Libraries, Gene --- Libraries, cDNA --- Library, DNA --- Library, Gene --- Library, cDNA --- cDNA Libraries --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Complementary DNA --- DNA, Complementary. --- Human genetics. --- Gene expression. --- Human Genetics. --- Gene Expression. --- Genes --- Genetic regulation --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Expression
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The first edition of Jim Watson's classic textbook Molecular Biology of the Gene appeared in 1965. This new edition, written with five new authors, has been brought fully up to date, and incorporates insights very recently derived from genome sequencing in a variety of organisms. The book is an authoritative and comprehensive survey of the fundamentals of molecular biology, from basic mechanisms to the elaborate role of gene regulation in embryonic development and evolution. Although updated, the twentyone chapters of the new edition retain the distinctive and celebrated features of the original work, including introductory chapters on the history of genetics and molecular biology and an emphasis throughout on the chemical underpinnings of molecular biology. The new team of authors brings to this edition awardwinning teaching experience and outstanding research achievements. By revealing the intellectual framework and experimental approaches that made new discoveries in the field possible, the new edition highlights the significance of the molecular approach for all of biology.
Molecular biology --- Molecular genetics --- Textbooks. --- Moleculaire biologie --- Moleculaire genetica --- Genetic Processes. --- Genetic Structures. --- Genetic Techniques. --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetic Counseling --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Processes --- Genetic Structures --- Genetic Techniques --- Molecular Biology --- Genetics --- Genetic Phenomena --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- methods --- 575/12 --- Cytogenetics --- genetica --- moleculaire biologie --- Biologie moléculaire --- Manuels d'enseignement supérieur
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DNA Viruses --- Genetic Structures --- Genetic Phenomena --- Vertebrate Viruses --- Viruses --- Organisms --- Phenomena and Processes --- Adenoviridae --- Genetic Vectors --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Bacteriophages --- DNA, Recombinant --- Plasmids --- Adenoviruses --- Ichtadenovirus --- Adenovirus --- Ichtadenoviruses --- Animal Viruses --- Zoophaginae --- Animal Virus --- Virus --- Virus, Animal --- Viruses, Animal --- Microbiology --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Molecular Biology --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetics --- DNA Virus --- Virus, DNA --- Viruses, DNA --- Genetic Therapy --- Genetic Vectors. --- methods. --- genetics.
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This monograph series is commissioned by the Phytochemical Society of North America (PSNA). The volumes in this series contain articles on developing topics of interest to scientists, students and individuals interested in recent developments in the biochemistry, chemistry and molecular biology of plants. Volume 36 centers on the role of phytochemistry in the rapid developments in biology brought about by the application of large-scale genomics approaches. Several functional genomic approaches discussed in this volume address plant gene function on a large scale. Plants are combinatori
Botanical chemistry. --- Botanical chemistry--Congresses. Plant genetics--Congresses. --- Chemistry. --- Congresses. --- Genetics. --- Genome, Plant. --- Plant biochemical genetics. --- Plant genetics. --- Plants. --- Eukaryota --- Genome --- Genetic Structures --- Organisms --- Genetic Phenomena --- Phenomena and Processes --- Plants --- Genome, Plant --- Botany --- Earth & Environmental Sciences --- Plant Physiology --- Plant Genome --- Genomes, Plant --- Plant Genomes --- Genes, Plant --- Plant --- Plant Proteins --- Ethnopharmacology --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Molecular Biology --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetics --- Genomes --- Genes --- Proteome --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Analog electronic systems. --- Electronic circuits.
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The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.
Medical microbiology, virology, parasitology --- Molecular biology --- Gene therapy --- Genetic vectors --- Transfection --- Viral genetics --- Virus-vector relationships --- Organisms --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Viruses --- Genetic Vectors --- Medicine --- Health & Biological Sciences --- Pathology --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Animal Viruses --- Zoophaginae --- Animal Virus --- Virus --- Virus, Animal --- Viruses, Animal --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Vector-virus relationships --- Virus genetics --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Therapy, Gene --- Genetics --- Human genetics. --- Medical virology. --- Gene therapy. --- Human Genetics. --- Virology. --- Gene Therapy. --- Medical microbiology --- Virology --- Virus diseases --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology
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This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.
Plant molecular genetics --- Plant gene expression --- Genomics --- Genome --- Eukaryota --- Gene Expression Regulation --- Organisms --- Genetic Processes --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Gene Expression Regulation, Plant --- Plants --- Genome, Plant --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Plant molecular genetics. --- Plant gene expression. --- Genomics. --- Génétique moléculaire végétale --- Plantes --- Génomique --- Expression génique --- Plant Genome --- Genomes, Plant --- Plant Genomes --- Plant --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Genomes --- Genome research --- Research --- Life sciences. --- Plant science. --- Botany. --- Life Sciences. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Biosciences --- Sciences, Life --- Science --- Floristic botany
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Gene Therapy --- Genetic Vectors --- Gene therapy --- Genetic vectors --- Thérapie génique --- Vecteurs de clonage --- Biochemistry --- Biotechnology --- Human genetics --- Medicine --- Microbiology --- Chemistry. --- Human genetics. --- Microbiology. --- Medicine. --- Biotechnology. --- Biochemistry. --- Human Genetics. --- Molecular Medicine. --- Medical Microbiology. --- Biochemistry, general. --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Biomedical Engineering --- Bioengineering --- Health & Biological Sciences --- Mechanical Engineering --- Engineering & Applied Sciences --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Clinical sciences --- Medical profession --- Microbial biology --- Composition --- Medical microbiology. --- Molecular biology. --- Biology --- Chemistry --- Medical sciences --- Chemical engineering --- Genetic engineering --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Physical sciences --- Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Microorganisms --- Life sciences --- Pathology --- Physicians --- Health Workforce
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In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.
Gene therapy --- Gene Transfer Techniques --- Genetic Vectors --- Genetic Techniques --- Genetic Structures --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Pathology --- Medicine --- Health & Biological Sciences --- Methodology --- Genetic Therapy --- Thérapie génique --- methods. --- Methodology. --- Méthodologie --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Therapy, Gene --- methods --- Human genetics. --- Animal genetics. --- Gene therapy. --- Human Genetics. --- Animal Genetics and Genomics. --- Gene Therapy. --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology
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