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This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.

Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Mosaicism. --- Neurons. --- Genomic Instability. --- Vertebrates --- Genome Instability --- Genome Stability --- Genomic Stability --- Genome Instabilities --- Genome Stabilities --- Genomic Instabilities --- Genomic Stabilities --- Instabilities, Genome --- Instabilities, Genomic --- Instability, Genome --- Instability, Genomic --- Stabilities, Genome --- Stabilities, Genomic --- Stability, Genome --- Stability, Genomic --- Chromothripsis --- DNA Sequence, Unstable --- Nerve Cells --- Cell, Nerve --- Cells, Nerve --- Nerve Cell --- Neuron --- Chimera --- Chimerism --- genetics.

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This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Trinucleotide Repeats. --- Genomic Instability. --- Triplet Repeats --- Repeat, Trinucleotide --- Repeat, Triplet --- Repeats, Trinucleotide --- Repeats, Triplet --- Trinucleotide Repeat --- Triplet Repeat --- Genome Instability --- Genome Stability --- Genomic Stability --- Genome Instabilities --- Genome Stabilities --- Genomic Instabilities --- Genomic Stabilities --- Instabilities, Genome --- Instabilities, Genomic --- Instability, Genome --- Instability, Genomic --- Stabilities, Genome --- Stabilities, Genomic --- Stability, Genome --- Stability, Genomic --- Chromothripsis --- DNA Sequence, Unstable

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All living things are remarkably complex, yet their DNA is unstable, undergoing countless random mutations over generations. Despite this instability, most animals do not grow two heads or die, plants continue to thrive, and bacteria continue to divide. Robustness and Evolvability in Living Systems tackles this perplexing paradox. The book explores why genetic changes do not cause organisms to fail catastrophically and how evolution shapes organisms' robustness. Andreas Wagner looks at this problem from the ground up, starting with the alphabet of DNA, the genetic code, RNA, and protein molecules, moving on to genetic networks and embryonic development, and working his way up to whole organisms. He then develops an evolutionary explanation for robustness. Wagner shows how evolution by natural selection preferentially finds and favors robust solutions to the problems organisms face in surviving and reproducing. Such robustness, he argues, also enhances the potential for future evolutionary innovation. Wagner also argues that robustness has less to do with organisms having plenty of spare parts (the redundancy theory that has been popular) and more to do with the reality that mutations can change organisms in ways that do not substantively affect their fitness. Unparalleled in its field, this book offers the most detailed analysis available of all facets of robustness within organisms. It will appeal not only to biologists but also to engineers interested in the design of robust systems and to social scientists concerned with robustness in human communities and populations.

Robust control --- Robust control. --- Genomic Instability. --- Mutation (Biology). --- Robustness (Control systems) --- Mutation. --- Evolution, Molecular. --- Biological systems --- Mutation (Biology) --- Molecular evolution. --- Genetic Evolution --- Molecular Evolution --- Evolution, Genetic --- Directed Molecular Evolution --- Genome Instability --- Genome Stability --- Genomic Stability --- Genome Instabilities --- Genome Stabilities --- Genomic Instabilities --- Genomic Stabilities --- Instabilities, Genome --- Instabilities, Genomic --- Instability, Genome --- Instability, Genomic --- Stabilities, Genome --- Stabilities, Genomic --- Stability, Genome --- Stability, Genomic --- Chromothripsis --- DNA Sequence, Unstable --- Mutations --- DNA Damage --- Polymorphism, Restriction Fragment Length --- Automatic control --- Biosystems --- Systems, Biological --- Biology --- System theory --- Systems biology --- Genetics --- Variation (Biology) --- Biochemical evolution --- Chemical evolution --- Evolution --- Life --- Molecular biology --- Stability. --- Philosophy --- Origin --- Molecular evolution --- Stability --- General biophysics --- Biological systems - Stability --- Acqui 2006