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Molecular biology techniques : a classroom laboratory manual
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ISBN: 128331097X 9786613310972 0123855454 0123855446 9780123855459 9780123855442 6613310972 9781283310970 Year: 2012 Publisher: Amsterdam ; Boston : Elsevier /Academic Press,

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Abstract

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester,


Book
Essential molecular biology : a practical approach
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ISBN: 019963114X 0199631158 0199631115 0199631107 0199631123 0199631131 Year: 1991 Publisher: Oxford : IRL Press,

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The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Volume II covers the procedures needed for identification of specific genes and the study of their structure and cellular expression. Topics covered include preparation of gene libraries, hybridization methods, nucleic acid sequencing, DNA mapping, and DNA amplification by the polymerase chain reaction. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book thus brings powerful techniques at the forefront of molecular biology research to the researcher. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.


Periodical
Bioengineered.
ISSN: 21655987 21655979 Year: 2012 Publisher: Austin, TX : Landes Bioscience,

Recombinant DNA
Authors: --- ---
ISBN: 0716719940 0716722828 9780716722823 Year: 1992 Publisher: New York: Scientific American Books,


Book
Genetic engineering: an introduction to gene analysis and exploitation in eukaryotes
Authors: ---
ISBN: 0632015217 0632015195 9780632015214 Year: 1988 Publisher: Oxford: Blackwell,

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Keywords

Biological techniques --- Molecular biology --- Biotechnology --- Eukaryotic cells. --- Genetic engineering. --- Cellules eucaryotes --- Génie génétique --- Genetic Engineering --- DNA, Recombinant --- Transformation, Genetic --- Cells --- Genetic engineering --- Eukaryotic cells --- genetic engineering --- Localisation de gène --- gene location --- Transfert de gène --- Gene transfer --- Cellule d'eucaryote --- eukaryotic cells --- Cells. --- DNA, Recombinant. --- Genetic Engineering. --- Transformation, Genetic. --- Genetic Transformation --- Genetic Transformations --- Transformations, Genetic --- Crosses, Genetic --- Transduction, Genetic --- Transfection --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Cloning, Molecular --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- Recombinant DNA --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- DNA Transposable Elements --- Genetic Vectors --- Genomic Library --- Gene Library --- Cell --- Cell Biology --- Génie génétique --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Eucaryotic cells --- Protista


Book
An introduction to recombinant DNA techniques : basic experiments in gene manipulation
Authors: --- ---
ISBN: 0805301380 9780805301380 Year: 1988 Publisher: Menlo Park, Calif.: Benjamin/Cummings,

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