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Book
Dictyostelium discoideum Protocols
Authors: ---
ISBN: 1627033017 1627033025 Year: 2013 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.   The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.   Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.

Keywords

Dictyostelium discoideum --- Models, Theoretical --- Publication Formats --- Dictyosteliida --- Biology --- Investigative Techniques --- Biological Science Disciplines --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Mycetozoa --- Natural Science Disciplines --- Amoebozoa --- Disciplines and Occupations --- Eukaryota --- Organisms --- Laboratory Manuals --- Models, Biological --- Dictyostelium --- Genetic Techniques --- Models, Genetic --- Genetics --- Health & Biological Sciences --- Microbiology & Immunology --- Cytogenetics --- Biologic Sciences --- Biological Science --- Science, Biological --- Sciences, Biological --- Biological Sciences --- Life Sciences --- Biologic Science --- Biological Science Discipline --- Discipline, Biological Science --- Disciplines, Biological Science --- Life Science --- Science Discipline, Biological --- Science Disciplines, Biological --- Science, Biologic --- Science, Life --- Sciences, Biologic --- Sciences, Life --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Dictyostelid Cellular Slime Molds --- Slime Molds, Cellular --- Cellular Slime Mold --- Cellular Slime Molds --- Dictyosteliidas --- Mold, Cellular Slime --- Molds, Cellular Slime --- Slime Mold, Cellular --- Experimental Model --- Experimental Models --- Mathematical Model --- Model, Experimental --- Models (Theoretical) --- Models, Experimental --- Models, Theoretic --- Theoretical Study --- Mathematical Models --- Model (Theoretical) --- Model, Mathematical --- Model, Theoretical --- Models, Mathematical --- Studies, Theoretical --- Study, Theoretical --- Theoretical Model --- Theoretical Models --- Theoretical Studies --- Genetic Models --- Genetic Model --- Model, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Dictyostelium discoideums --- Dictyosteliums --- discoideum, Dictyostelium --- Natural Sciences --- Physical Sciences --- Discipline, Natural Science --- Disciplines, Natural Science --- Natural Science --- Natural Science Discipline --- Physical Science --- Science, Natural --- Science, Physical --- Sciences, Natural --- Sciences, Physical --- Biological Model --- Biological Models --- Model, Biological --- Models, Biologic --- Biologic Model --- Biologic Models --- Model, Biologic --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- methods --- Eumycetozoa --- Eumycetozoas --- Mycetozoas --- Life sciences. --- Microbiology. --- Life Sciences. --- Eukaryotic Microbiology. --- Microbial biology --- Microorganisms --- Biosciences --- Science


Book
The internet is not what you think it is : a history, a philosophy, a warning
Author:
ISBN: 9780691229683 0691229686 Year: 2022 Publisher: Princeton, New Jersey : Princeton University Press,

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Abstract

An original deep history of the internet that tells the story of the centuries-old utopian dreams behind it--and explains why they have died today. Many think of the internet as an unprecedented and overwhelmingly positive achievement of modern human technology. But is it? In The Internet Is Not What You Think It Is, Justin Smith offers an original deep history of the internet, from the ancient to the modern world--uncovering its surprising origins in nature and centuries-old dreams of radically improving human life by outsourcing thinking to machines and communicating across vast distances. Yet, despite the internet's continuing potential, Smith argues, the utopian hopes behind it have finally died today, killed by the harsh realities of social media, the global information economy, and the attention-destroying nature of networked technology. Ranging over centuries of the history and philosophy of science and technology, Smith shows how the "internet" has been with us much longer than we usually think. He draws fascinating connections between internet user experience, artificial intelligence, the invention of the printing press, communication between trees, and the origins of computing in the machine-driven looms of the silk industry. At the same time, he reveals how the internet's organic structure and development root it in the natural world in unexpected ways that challenge efforts to draw an easy line between technology and nature. Combining the sweep of intellectual history with the incisiveness of philosophy, The Internet Is Not What You Think It Is cuts through our daily digital lives to give a clear-sighted picture of what the internet is, where it came from, and where it might be taking us in the coming decades.

Keywords

Internet. --- Technology --- Technology and civilization. --- Civilization and machinery --- Civilization and technology --- Machinery and civilization --- Civilization --- Social history --- DARPA Internet --- Internet (Computer network) --- Wide area networks (Computer networks) --- World Wide Web --- Social aspects. --- Philosophy --- Abstraction. --- Analogy. --- Analytical Engine. --- Anthropomorphism. --- Antithesis. --- Artificial general intelligence. --- Artificial intelligence. --- Artificial language. --- Artificial life. --- Artificial stupidity. --- Artificiality. --- Attention deficit hyperactivity disorder. --- Automaton. --- Binary code. --- Bullying. --- Caricature. --- Causality. --- Columnist. --- Computer science. --- Computer virus. --- Computer. --- Computing. --- Consciousness. --- Conspiracy theory. --- Controversy. --- Copyright. --- Counterfeit. --- Criticism. --- Curtailment. --- Cyberculture. --- Cybernetics. --- Declamation. --- Detriment (astrology). --- Dichotomy. --- Disputation. --- Distraction. --- Doubt. --- Dystopia. --- Evocation. --- Explanation. --- External storage. --- Fantasy literature. --- Fungus. --- Heresy. --- Hypothesis. --- Imposition. --- Indictment. --- Information overload. --- Information revolution. --- Instance (computer science). --- Intellectual property. --- Internet troll. --- Irony. --- Lie. --- LinkedIn. --- Materialism. --- Naked eye. --- Negation. --- Obesity. --- Ontology (information science). --- Opportunism. --- Outsourcing. --- Overlay network. --- Perception. --- Perversion. --- Pessimism. --- Philosophy. --- Police brutality in the United States. --- Police brutality. --- Printing. --- Processing (programming language). --- Protest. --- Punched card. --- Racism. --- Radicalization. --- Reason. --- Resentment. --- Robbery. --- Scarcity (social psychology). --- Sentience. --- Simulation hypothesis. --- Simulation. --- Slang. --- Slavery. --- Slime mold. --- State of nature. --- Subject (philosophy). --- Syllogism. --- Technology. --- Telecommunication. --- Terminology. --- Theory. --- Thought. --- Tinder (app). --- Twitter. --- Uncertainty. --- Understanding. --- Vandalism. --- Virtual world. --- Writing. --- Internet --- Artificial intelligence --- history. --- History. --- PHILOSOPHY / Ethics & Moral Philosophy. --- PHILOSOPHY / History & Surveys / Modern. --- PHILOSOPHY / Social. --- COMPUTERS / Internet / Online Safety & Privacy. --- COMPUTERS / Internet / Social Media.

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