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Molecular Methods in Developmental Biology : Xenopus and Zebrafish
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ISBN: 0896037908 9786610831531 1280831537 1592596789 Year: 1999 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.


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Xenopus Protocols : Post-Genomic Approaches
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ISBN: 1617799920 1617799912 9781617799914 Year: 2012 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  .

Keywords

Xenopus --- Investigative Techniques --- Pipidae --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anura --- Amphibians --- Vertebrates --- Chordata --- Animals --- Eukaryota --- Organisms --- Genetic Techniques --- Zoology --- Health & Biological Sciences --- Embryology --- Laboratory manuals --- Embriologie --- Manuels de laboratoire --- Bombina --- Frogs and Toads --- Salientia --- Toad, Fire-Bellied --- Toads and Frogs --- Anuras --- Fire-Bellied Toad --- Fire-Bellied Toads --- Salientias --- Toad, Fire Bellied --- Toads, Fire-Bellied --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Chordates --- Chordate --- Vertebrate --- Amphibia --- Amphibian --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- African clawed frog --- Dactylethra --- Silurana --- methods --- Medicine. --- Human genetics. --- Animal genetics. --- Animal models in research. --- Biomedicine. --- Human Genetics. --- Animal Genetics and Genomics. --- Animal Models. --- Animal experimentation --- Biological models --- Laboratory animals --- Research --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Laboratory animals. --- Animals, Experimental --- Animals, Laboratory --- Animals in research --- Experimental animals --- Lab animals --- Animal culture --- Laboratory organisms --- Working animals

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