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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptorsContains chapters
Cell receptors -- Research -- Methodology. --- Cellular signal transduction -- Research -- Methodology. --- G proteins -- Receptors. --- Signal transduction -- Physiology -- Laboratory manuals. --- Metabolism --- Biological Transport --- Receptors, Cell Surface --- Biochemical Processes --- Membrane Proteins --- Biochemical Phenomena --- Chemical Processes --- Metabolic Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Receptors, G-Protein-Coupled --- Protein Transport --- Protein Binding --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- G proteins --- Receptors. --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Enzymology.
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Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.
Cellular signal transduction --- G proteins --- Cell receptors --- GTP-Binding Proteins --- Signal Transduction --- analysis --- physiology --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cellular signal transduction - Laboratory manuals --- G proteins - Laboratory manuals --- Cell receptors - Laboratory manuals --- GTP-Binding Proteins - analysis - laboratory manuals --- Signal Transduction - physiology - Laboratory Manuals
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