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This third edition expands upon the previous edition with current, detailed methods on different aspects, and landmarks advancing knowledge on Salmonella research. With new chapters on molecular assays for detection, identification and serotyping of Salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Salmonella: Methods and Protocols, Third Edition aims to provide methods that will have an impacted Salmonella research.

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This volume discusses the latest protocols used by scientists to understand ZIKV and how to mitigate its presence. These studies will benefit in the detection, diagnosis, management, treatment, and prevention of this infection. The chapters in this book cover techniques in virus isolation, purification, and ways to titrate the virus; recent advancements made in the development of modified molecular techniques; and a discussion on Zika virus itself to help readers better understand this pathogen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic. .

Virology. --- Diseases. --- Human beings --- Illness --- Illnesses --- Morbidity --- Sickness --- Sicknesses --- Medicine --- Epidemiology --- Health --- Pathology --- Sick --- Microbiology --- Diseases

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This textbook is specifically written for clinicians involved in managing patients with dyspepsia. It is a practical guide with up-to-date suggestions on evaluation, diagnosis, and management from experts from around the world. Each chapter is a succinct review of current topics that play a role in the pathogenesis and management of this disorder. Special populations such as pediatrics, those with cardiovascular disease and womens health are specifically examined.

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Diseases --- Human beings --- Illness --- Illnesses --- Morbidity --- Sickness --- Sicknesses --- Medicine --- Epidemiology --- Health --- Pathology --- Sick --- Treatment. --- Treatment

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This volume details the phenotypic characterization of Staphylococcus aureus, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Staphylococcus aureus: Methods and Protocols is a valuable resource for anyone interested in this fascinating and developing field.

Diseases. --- Human beings --- Illness --- Illnesses --- Morbidity --- Sickness --- Sicknesses --- Medicine --- Epidemiology --- Health --- Pathology --- Sick --- Diseases