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This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the “activation barrier” when undertaking a new discipline, and provides a “toolbox” for the next generation of ADC scientists.

Immunospecificity. --- Pharmacology. --- Adaptive Immunity. --- Drug effects --- Medical pharmacology --- Medical sciences --- Chemicals --- Chemotherapy --- Drugs --- Pharmacy --- Immunological specifics --- Serological specificity --- Specificity (Immunology) --- Antibody diversity --- Antigenic determinants --- Binding sites (Biochemistry) --- Immune recognition --- Physiological effect

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Antigen-Antibody Reactions. --- Enzymes --- Immunochemistry. --- Antigen-antibody reactions --- -Immunochemistry --- Immunospecificity --- Immunological specifics --- Serological specificity --- Specificity (Immunology) --- Antibody diversity --- Antigenic determinants --- Binding sites (Biochemistry) --- Immune recognition --- Biochemistry --- Immunity --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology --- Antibody-antigen reactions --- Antigens --- Immune response --- Immunoglobulins --- Immunology --- Immunoblotting --- immunology. --- Analysis --- Immunochemistry --- Antigen-Antibody Reactions --- Antigen Antibody Reactions --- Antigen-Antibody Reaction --- Reaction, Antigen-Antibody --- Reactions, Antigen-Antibody --- AC133 Antigen --- Antibodies --- immunology

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This book provides an essential overview of the role of phosphoinositide-3-phosphate kinase (PI3K) isoforms in modulating the function of immune system cells and their involvement in disease. PI3K is a family of kinases involved in basic cellular processes such as proliferation, migration and cell death. Recent work has highlighted the multiple roles of PI3K classes and subunits and their involvement in the immune response to the body’s own and foreign antigens and diseases such as cancer and autoimmunity. This book offers a detailed introduction to the biology of the three PI3K classes, followed by an extensive discussion of the diverse roles of AKT and PI3K isoforms in immune cells. Based on this knowledge, it subsequently explains in more detail how specific isoforms are connected to immune-mediated diseases. The book concludes by highlighting the latest advances in the production of isoform-specific inhibitors and their use in various human diseases. This book is intended as a reference guide for students and researchers interested in the multifaceted aspects of PI3K biology.

Molecular immunology. --- Phosphoinositides. --- Immune response. --- Immunology --- Phosphatidylinositides --- Phosphatidylinositol phosphates --- Phospholipids --- Inositol --- Immune system --- Immunity --- Molecular biology --- Molecular aspects --- Immunology. --- Immune system. --- Cellular signal transduction. --- Cytology. --- Autoimmunity. --- Immunospecificity. --- Immune Cell Signalling. --- Cell Biology. --- Adaptive Immunity. --- Immunological specifics --- Serological specificity --- Specificity (Immunology) --- Antibody diversity --- Antigenic determinants --- Binding sites (Biochemistry) --- Immune recognition --- Immunobiology --- Life sciences --- Serology --- Autoallergy --- Autoantibodies --- Cell biology --- Cellular biology --- Biology --- Cells --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Immunological system --- Anatomy

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This volume examines all facets of the complex biology of Immunoglobulin E (IgE) antibodies, which play an essential role in the pathophysiology of allergic diseases and immunity to parasites. It highlights the unique mechanisms involved in the regulation of IgE production at both the molecular and cellular level. Furthermore, it discusses in detail novel findings on how the affinity, specificity and cross-reactivity of IgE can fine-tune mast cell responses to allergens. The book also explores the beneficial roles of IgE antibodies in immunity to helminthes and protection against tumors, and how the properties of IgE-mediated immunity are employed in the development of IgE therapeutic antibodies. All chapters were written by respected experts in their fields and will appeal to scientists and clinicians alike.

Biomedicine. --- Antibodies. --- Molecular Medicine. --- Allergology. --- Parasitology. --- Medicine. --- Monoclonal antibodies. --- Medical parasitology. --- Allergy. --- Médecine --- Anticorps monoclonaux --- Parasitologie médicale --- Allergie --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Immunoglobulin E --- Immunoglobulin G --- Receptors. --- Analysis. --- IgE --- IgG --- Molecular biology. --- Allergic diseases --- Allergies --- Hypersensitivity --- Hypersensitivity, Immediate --- Immediate allergy --- Immediate hypersensitivity --- Immunologic diseases --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Antibodies --- Immune globulins --- Immune serum globulin --- Blood proteins --- Globulins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Immunoglobulins --- Allergy --- Anti-immunoglobulin autoantibodies --- Human beings --- Human parasitology --- Parasitology --- Parasitic diseases --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Molecular cloning --- Parasites --- Health Workforce --- Immunospecificity. --- Medicine --- Adaptive Immunity. --- Biomedical Research. --- Research. --- Biological research --- Biomedical research --- Immunological specifics --- Serological specificity --- Specificity (Immunology) --- Antigenic determinants --- Binding sites (Biochemistry) --- Immune recognition

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En 2008, en France, 222 patients sont décédés faute d'avoir reçu un greffon à temps, alors que seulement 4 620 greffes avaient été réalisées pour 13 687 personnes en attente. La pénurie est particulièrement aiguë pour les malades qui ont besoin d'un rein : on compte en moyenne seulement 1 greffon disponible pour 3 malades. Cette situation est assez générale en Europe, mais moins grave dans certains pays qui ont su développer, de différentes manières, une plus grande mobilisation des deux sources de greffons : les donneurs décédés et les donneurs vivants. Le séminaire qui s'est tenu à Paris les 6 et 7 mai 2009 s'est proposé de contribuer à la réflexion des autorités en charge de la révision des lois de bioéthique ainsi que, plus généralement, des milieux médicaux et de l'opinion publique, en apportant un éclairage européen sur les solutions possibles au déficit constaté. Des personnalités faisant autorité - médecins et chirurgiens, mais aussi juristes, sociologues, philosophes et responsables administratifs venant d'Allemagne, d'Espagne, d'Italie, de Grande-Bretagne, de Norvège, des Pays-Bas et de Suède - ont été invitées à présenter leurs expériences et à débattre des aspects éthiques associés, en présence de leurs homologues français et de responsables politiques. Cet ouvrage transcrit les interventions et les débats, souvent vifs, qui les ont suivies. Penser la greffe aujourd'hui, c'est l'envisager à travers les enjeux de la société. Face aux certitudes médicales, chaque individu perçoit des incertitudes éthiques. Les défis de la science imposent la transparence, garantie d'une " nouvelle démocratie ". La greffe apparaît alors ambivalente, dans son fondement philosophique, juridique et social, comme dans sa pratique chirurgicale. Faut-il parler de don altruiste ou d'échange réciproque à propos des " éléments et produits du corps humain " ? Dans le cas d'un prélèvement sur une personne décédée, la mort devient-elle " utile " parce qu'utilisée ? S'agit-il de mutiler pour réparer, ou de reconstruire l'homme et de repousser ainsi les limites de la vie ? Sur le terrain, le nombre important des refus de prélèvements induit-il vraiment une " pénurie " d'organes ? Faut-il l'attribuer au manque d'information du public, à la méfiance à l'égard des médecins, à l'attachement à des valeurs personnelles, aux droits des familles, à l'incontournable présence de l'Etat ? Le législateur, depuis l'élaboration des lois de bioéthique jusqu'à leur application réglementaire encore inachevée, a tenté de réaliser un cadre protecteur pour la personne humaine. Y est-il parvenu ? Ces débats d'idées ainsi que les résultats inattendus de la première enquête nationale sur le don d'organes constituent le point le plus complet à ce jour sur le sujet du prélèvement et de la greffe. Ce livre est né de la volonté d'éclairer les citoyens et d'attirer l'attention des politiques sur ce constat : la greffe existe, elle se doit de rester humaine.

Transplantation of organs, tissues, etc. --- Medical ethics --- Greffe (Chirurgie) --- Ethique médicale --- Moral and ethical aspects --- Aspect moral --- Donation of organs, tissues, etc --- Transplantation of organs, tissues, etc --- Immunospecificity --- Donation of organs, tissues, etc. --- Immunospecificity. --- Transplantation --- Government Regulation --- Social Conditions --- orgaantransplantatie (allocatie van organen, donorschaarste) --- Frankrijk --- Immunological specifics --- Serological specificity --- Specificity (Immunology) --- Antibody diversity --- Antigenic determinants --- Binding sites (Biochemistry) --- Immune recognition --- Medical transplantation --- Organ transplantation --- Organ transplants --- Organs (Anatomy) --- Surgical transplantation --- Tissue transplantation --- Tissues --- Transplants, Organ --- Surgery --- Preservation of organs, tissues, etc. --- Procurement of organs, tissues, etc. --- Government Regulation and Oversight --- Government Regulations --- Regulation, Government --- Regulations, Government --- Transplantations --- Transplantation Immunology --- Transplantation Conditioning --- Anatomical gifts --- Organ donation --- Tissue donation --- ethics --- history --- transplantation d'organes (greffe d'organes, pénurie d'organes, allocation d'organes) --- France --- Donation --- Human transplant --- Socio-ethics --- Ethique médicale --- Transplant surgery --- Transplantation surgery --- Living Conditions --- Condition, Living --- Condition, Social --- Conditions, Living --- Conditions, Social --- Living Condition --- Social Condition --- Donation of organs, tissues, etc - Moral and ethical aspects - France --- Transplantation of organs, tissues, etc - Moral and ethical aspects - France