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Dissertation
Peut-on établir une relation entre la qualité du sperme et l'hétérozygotie afin d'améliorer notre approche dans la conservation des mammifères sauvages menacés ? Exemple par l'analyse de quatre canidés
Authors: --- --- --- --- --- et al.
Year: 2019 Publisher: Liège Université de Liège (ULiège)

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Abstract

La conservation de la biodiversité et l’impact de l’être humain sur celle-ci est au centre de nos préoccupations depuis quelques années. La génomique est une arme puissante qui, associée à de nombreux domaines, permet des avancées considérables. La compréhension du déclin de certaines espèces sauvages et ses répercussions sur le génome et la reproduction, font l’objet de recherches. Dans ce travail, les principes de l’analyse de la qualité de la semence et les principes de la génomique seront abordés. Par la suite, un lien entre ces deux grands domaines sera établi afin de l’appliquer à la problématique de la conservation animale. En effet, si la consanguinité est bien négativement corrélée avec le succès de la reproduction, les causes et les conséquences exactes régissant ce lien ne sont pas claires. C’est pourquoi, à travers l’hypothèse que le degré d’hétérozygotie influence certains paramètres de qualité du sperme, le lien établi entre consanguinité et reproduction peut potentiellement être décodé. Cette hypothèse sera testée sur quarante-six espèces menacées et cinq espèces de références. Enfin, quatre espèces de canidés seront ensuite prises pour exemple. Les résultats obtenus permettent de confirmer statistiquement le lien entre l’hétérozygotie et le pourcentage de spermatozoïdes normaux mais réfutent le lien entre hétérozygotie et motilité. Dans cette étude, un lien est également établi entre l’hétérozygotie et la concentration en spermatozoïdes de l’éjaculat. Il serait donc nécessaire de poursuivre et approfondir les études avant de pouvoir tirer certaines conclusions. The conservation of biodiversity and the impact of the human being on it has been at the center of our concerns for a few years now. Genomics is a powerful weapon that, combined with many disciplinary fields, allows for significant advances. Understanding the decline of some wildlife species and its implications on the genome and reproduction are being studied. In this work, the principles of semen quality analysis and the principles of genomics will be discussed. Subsequently, a link between these two main areas will be established in order to apply it to the issue of animal conservation. Indeed, if consanguinity is correlated with the success of reproduction, the exact causes and consequences governing this link are unclear. Therefore, assuming that the degree of heterozygosity influences certain sperm quality parameters, the link established between consanguinity and reproduction can potentially be decoded. This hypothesis will be tested on forty-six threatened species and five reference species. Finally, four species of canids will then be taken as an example. The results obtained statistically confirm the link between heterozygosity and the percentage of normal spermatozoa but refute the link between heterozygosity and motility. In this study, a link is also established between heterozygosity and the concentration of spermatozoa in the ejaculate. It would therefore be necessary to continue and deepen the studies before drawing certain conclusions.


Book
A practical guide to basic laboratory andrology
Authors: --- --- --- ---
ISBN: 1009181645 1009181637 1009191241 100919125X Year: 2022 Publisher: Cambridge : Cambridge University Press,

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This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes quality control considerations and the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. The interpretation of laboratory results in the clinical context is highlighted throughout, and safe laboratory practice is emphasized. Fully revised, incorporating the new ISO TS 23162 on basic human semen analysis throughout, this is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.


Book
Challenges and New Strategies on Rabbit Breeding
Authors: ---
Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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Rabbit livestock is especially important in the Mediterranean area and in the semi-arid zones of Africa, Asia, and America, representing a valuable alternative source of good animal protein of high quality (low fat and low cholesterol). However, rabbit husbandry should adapt to be a competitive and sustainable sector. In addition, it must maintain a balance between rabbit welfare, customer demands, and breeder requirements. Then, improvement in breeding systems is needed and may involve avoiding hormones and antibiotics in rabbit management; enhancing the efficiency of reproductive procedures such as artificial insemination systems and freezing of semen and embryos; extending the productive lifespan of does; improving genetic selection systems; and adjusting nutritional systems to optimize the physiological status of animals and the immune response. This Special Issue concerns all these challenges in increasing rabbit productivity and sustainability.


Book
Challenges and New Strategies on Rabbit Breeding
Authors: ---
Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Rabbit livestock is especially important in the Mediterranean area and in the semi-arid zones of Africa, Asia, and America, representing a valuable alternative source of good animal protein of high quality (low fat and low cholesterol). However, rabbit husbandry should adapt to be a competitive and sustainable sector. In addition, it must maintain a balance between rabbit welfare, customer demands, and breeder requirements. Then, improvement in breeding systems is needed and may involve avoiding hormones and antibiotics in rabbit management; enhancing the efficiency of reproductive procedures such as artificial insemination systems and freezing of semen and embryos; extending the productive lifespan of does; improving genetic selection systems; and adjusting nutritional systems to optimize the physiological status of animals and the immune response. This Special Issue concerns all these challenges in increasing rabbit productivity and sustainability.


Book
Challenges and New Strategies on Rabbit Breeding
Authors: ---
Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Rabbit livestock is especially important in the Mediterranean area and in the semi-arid zones of Africa, Asia, and America, representing a valuable alternative source of good animal protein of high quality (low fat and low cholesterol). However, rabbit husbandry should adapt to be a competitive and sustainable sector. In addition, it must maintain a balance between rabbit welfare, customer demands, and breeder requirements. Then, improvement in breeding systems is needed and may involve avoiding hormones and antibiotics in rabbit management; enhancing the efficiency of reproductive procedures such as artificial insemination systems and freezing of semen and embryos; extending the productive lifespan of does; improving genetic selection systems; and adjusting nutritional systems to optimize the physiological status of animals and the immune response. This Special Issue concerns all these challenges in increasing rabbit productivity and sustainability.


Book
The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports
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Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Nowadays, assisted reproductive technologies (ARTs) have a pivotal role not only in achieving fertilization in subfertile animals, but they are also involved in the management of the herd, decreasing disease spread and even allowing offspring sex selection. Nonetheless, there are differences between species or even within species that have led researchers worldwide to focus on those differences in order to bypass these specific difficulties. This Special Issue, titled “The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports” and published in Animals, is composed of 12 original manuscripts and three reviews that offer an overview of current and future ARTs used to improve reproductive outcomes, mainly focused on farm animals, such as horse, pig, bovine, rabbit and ovine species. Thus, the Special Issue covers information from the classical point of view, including comparative studies of different semen extenders, to the most advanced technologies of sperm selection by thermotaxis or chemoattractants, as well as the improvement of sperm features by red light irradiation. The female and embryo contributions to ART outcomes are also covered, for instance, with a study that improves our knowledge by the metabolomic description of follicular fluid composition or the description of better culture conditions of oocytes. In brief, this Special Issue provides a balanced overview of emerging techniques and technologies used to preserve, improve, rescue or even create fertility for domestic farm animals with high economic impact.


Book
The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports
Author:
Year: 2021 Publisher: Basel, Switzerland MDPI - Multidisciplinary Digital Publishing Institute

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Abstract

Nowadays, assisted reproductive technologies (ARTs) have a pivotal role not only in achieving fertilization in subfertile animals, but they are also involved in the management of the herd, decreasing disease spread and even allowing offspring sex selection. Nonetheless, there are differences between species or even within species that have led researchers worldwide to focus on those differences in order to bypass these specific difficulties. This Special Issue, titled “The Era of Assisted Reproductive Technologies Tailored to the Specific Necessities of Species, Industry and Case Reports” and published in Animals, is composed of 12 original manuscripts and three reviews that offer an overview of current and future ARTs used to improve reproductive outcomes, mainly focused on farm animals, such as horse, pig, bovine, rabbit and ovine species. Thus, the Special Issue covers information from the classical point of view, including comparative studies of different semen extenders, to the most advanced technologies of sperm selection by thermotaxis or chemoattractants, as well as the improvement of sperm features by red light irradiation. The female and embryo contributions to ART outcomes are also covered, for instance, with a study that improves our knowledge by the metabolomic description of follicular fluid composition or the description of better culture conditions of oocytes. In brief, this Special Issue provides a balanced overview of emerging techniques and technologies used to preserve, improve, rescue or even create fertility for domestic farm animals with high economic impact.

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