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Kluyveromyces --- Saccharomycetales --- growth & development

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Saccharomycetales --- Clinical Laboratory Techniques --- Saccharomycétales --- Laboratory Manuals. --- methods --- Laboratory Manuals --- Manuels de laboratoire --- Gisten --- Fermentatie --- Schimmels

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This volume describes recent advances in the bioconversion of lignocellulosics. It starts with two articles on genetics and properties of cellulases and their re- tion kinetics and mechanisms. The cost of cellulases has been a hindrance to large scale use of enzymatic hydrolysis. Two articles on cellulase production by submerged fermentation and by solid state fementation are included to describe the state of the art in this area. Dilute acid hydrolysis of cellulose continues to be of interest as well as potentially useful. The most recent advances in this area is also covered. A great deal of progress has been made in genetic engineering for improved regulation of xylose fermentation by yeasts. An article on genetically engineered Saccharomyces for simulteaneous fermentation of glucose and xylose describes the importance advances made in production of fuel ethanol from lignocellulosic biomass. In recent years, there has been increasing interests in recycling and the reuse of scrap paper as well as environment considerations. A contribution is presented which describes the research perspectives in that area. Finally, recent advances in the use of lignocellulosic biomass for the p- duction of ethanol and organic acids are presented in two articles. Renewable resources are inevitably of great importance in the years to come. There is a never-ending search for better living conditions for human beings. The more resource materials can be recycled, the richer we will be.

biomassa --- fermentatie --- General biochemistry --- biotechnologie --- hydrolyse --- recyclage --- Biochemical engineering --- Biotechnology --- biochemistry --- biochemie --- recycling --- probiotics --- cellulose --- bioengineering --- Biocarburants --- Bioenergy --- Biofuels --- Biological fuels --- Biomass energy --- Biomassa-energie --- Carburants verts --- Energie de la biomasse --- Energy [Biomass ] --- Energy conversion [Microbial ] --- Microbial energy conversion --- Chemistry --- Microbiology --- Lignocellulose--Biodégradation --- Lignocellulose--Biotechnologie --- 547.458.84 --- 577.152.321 --- 579.66 --- Lignocellulose. Other crude natural celluloses --- Glycoside hydrolases: glucosidases, amylase, cellulase --- Industrial and chemical microbiology --- Fermentation --- Cellulase --- Xylose --- Saccharomyces cerevisiae --- Genetic Engineering --- Carbohydrate Metabolism --- Genetic Techniques --- Pentoses --- Cellulases --- Saccharomyces --- Metabolism --- Yeasts --- Saccharomycetales --- Biochemical Processes --- Glucosidases --- Monosaccharides --- Investigative Techniques --- Chemical Processes --- Carbohydrates --- Metabolic Phenomena --- Fungi --- Biochemical Phenomena --- Ascomycota --- Glycoside Hydrolases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Hydrolases --- Eukaryota --- Phenomena and Processes --- Chemical Phenomena --- Enzymes --- Organisms --- Enzymes and Coenzymes --- 579.66 Industrial and chemical microbiology --- 577.152.321 Glycoside hydrolases: glucosidases, amylase, cellulase --- 547.458.84 Lignocellulose. Other crude natural celluloses --- Lignocellulose. --- Lignocellulose --- Biodegradation --- Biotechnology. --- Chemical engineering. --- Microbiology. --- Industrial Chemistry/Chemical Engineering. --- Microbial biology --- Biology --- Microorganisms --- Chemistry, Industrial --- Engineering, Chemical --- Industrial chemistry --- Engineering --- Chemistry, Technical --- Metallurgy --- Chemical engineering --- Genetic engineering --- Biodegradation. --- Genetics. --- Endo-1,4-beta-glucanase --- Glucanase, Endo-1,4-beta --- Glycosidases --- Wood

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In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Pichia --- Recombinant Proteins --- Recombinant proteins --- Saccharomyces cerevisiae --- Yeast fungi --- Yeasts --- biosynthesis --- Biotechnology --- Fungi --- Proteins --- Publication Formats --- Saccharomyces --- Saccharomycetales --- Publication Characteristics --- Ascomycota --- Eukaryota --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Organisms --- Laboratory Manuals --- Health & Biological Sciences --- Biomedical Engineering --- Baker Yeast --- S cerevisiae --- Baker's Yeast --- Brewer's Yeast --- Yeast, Baker's --- Yeast, Brewer's --- Baker Yeasts --- Baker's Yeasts --- Bakers Yeast --- Brewer Yeast --- Brewer's Yeasts --- Brewers Yeast --- Yeast, Baker --- Yeast, Bakers --- Yeast, Brewer --- Yeast, Brewers --- Yeasts, Baker --- Yeasts, Baker's --- Yeasts, Brewer's --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Hansenula --- Hansenulas --- Pichias --- Yeast --- Genetically engineered proteins --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Ascomycetes --- Cochliobolus --- Sclerotinia --- Ascomycete --- Ascomycotas --- Sclerotinias --- Budding Yeast --- Endomycetales --- Endomycopsis --- Yeast, Budding --- Budding Yeasts --- Endomycetale --- Endomycopses --- Saccharomycetale --- Yeasts, Budding --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Fungi, Filamentous --- Molds --- Filamentous Fungi --- Filamentous Fungus --- Fungus --- Fungus, Filamentous --- Mold --- Genetic engineering --- Saccharomyce --- Medicine. --- Human genetics. --- Biomedicine. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Pathology --- Physicians