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This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Human genetics. --- RNA Viruses --- Reverse Genetics --- Coronavirus --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- genetics --- methods --- genetics. --- methods.

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Pulmonary arterial hypertension is a severe and progressive disorder affecting the blood vessels in the lungs. Typically, symptoms first appear at around 30–40 years of age and, without treatment, can lead to fatal heart disease within a few years. Genetic studies over the past decade have identified numerous genes that contribute to disease progression but, for many sufferers, the underlying genetic cause remains elusive. The collection of reviews and original research articles contained within this book provide an overview of recent advancements in understanding the genetic risk factors for pulmonary arterial hypertension. We further examine the emerging interplay between genetic variants and clinical outcomes, providing a framework for new treatments and improved patient care.

Research & information: general --- Biology, life sciences --- Genetics (non-medical) --- pulmonary arterial hypertension --- massive parallel sequencing --- NGS --- digenic inheritance --- and genetics --- BMPR2 promoter --- pathogenic variant --- heritable pulmonary arterial hypertension --- genetic analysis --- NGS gene panel --- BMPR2 --- TBX4 --- GDF2 --- EIF2AK4 --- genomics --- pediatrics --- lung disease --- endothelial cells --- smooth muscle cells --- DNA damage --- DNA repair --- expression quantitative trait locus --- eQTL --- blood --- genetics --- exome sequencing --- molecular genetics --- paediatrics --- bone morphogenetic protein receptor type 2 --- heritable --- familial --- estrogen --- estradiol --- penetrance --- gender --- PAH --- forward phenotyping --- forward genetics --- reverse genetics --- reverse phenotyping --- intermediate phenotypes --- whole-genome sequencing --- epigenetic inheritance --- genetic heterogeneity --- phenotypic heterogeneity --- pulmonary hypertension --- bone morphogenetic protein receptor 2 --- signaling --- repurposed drugs --- pharmaceuticals --- miRNA --- clinical trials --- n/a

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Pulmonary arterial hypertension is a severe and progressive disorder affecting the blood vessels in the lungs. Typically, symptoms first appear at around 30–40 years of age and, without treatment, can lead to fatal heart disease within a few years. Genetic studies over the past decade have identified numerous genes that contribute to disease progression but, for many sufferers, the underlying genetic cause remains elusive. The collection of reviews and original research articles contained within this book provide an overview of recent advancements in understanding the genetic risk factors for pulmonary arterial hypertension. We further examine the emerging interplay between genetic variants and clinical outcomes, providing a framework for new treatments and improved patient care.

Research & information: general --- Biology, life sciences --- Genetics (non-medical) --- pulmonary arterial hypertension --- massive parallel sequencing --- NGS --- digenic inheritance --- and genetics --- BMPR2 promoter --- pathogenic variant --- heritable pulmonary arterial hypertension --- genetic analysis --- NGS gene panel --- BMPR2 --- TBX4 --- GDF2 --- EIF2AK4 --- genomics --- pediatrics --- lung disease --- endothelial cells --- smooth muscle cells --- DNA damage --- DNA repair --- expression quantitative trait locus --- eQTL --- blood --- genetics --- exome sequencing --- molecular genetics --- paediatrics --- bone morphogenetic protein receptor type 2 --- heritable --- familial --- estrogen --- estradiol --- penetrance --- gender --- PAH --- forward phenotyping --- forward genetics --- reverse genetics --- reverse phenotyping --- intermediate phenotypes --- whole-genome sequencing --- epigenetic inheritance --- genetic heterogeneity --- phenotypic heterogeneity --- pulmonary hypertension --- bone morphogenetic protein receptor 2 --- signaling --- repurposed drugs --- pharmaceuticals --- miRNA --- clinical trials

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This book includes a collection of eight original research articles and three reviews covering a wide range of topics in the field of kinetoplastids. In addition, readers can find a compendium of molecular biology procedures and bioinformatics tools.

trypanosomatids --- yeasts --- trypanosomatids genome --- cell cycle phases --- S-phase duration --- DNA replication --- replication origins --- neglected tropical diseases --- Leishmania --- Trypanosoma cruzi --- Trypanosoma brucei --- drug discovery --- in vitro models --- in vivo models --- genomics --- drug resistance --- Leishmania infantum --- proteome --- post-translational modifications (PTMs) --- proteogenomics --- mass spectrometry --- Retrotransposon Hot Spot (RHS) multigene family --- chromosome distribution --- recombination --- gene mosaic structure --- evolution --- nuclear protein --- Leishmania braziliensis --- reverse genetics --- CRISPR–Cas9 --- gene targeting --- phenotyping --- heat shock proteins --- Trypanosoma cruzi strain --- sequencing methods --- genome plasticity --- gene expression --- trans-sialidases --- mucins --- SENP --- Ulp2 --- SUMO --- CRISPR --- protease --- genome --- repeats --- 3′UTR --- multigenic family --- Leishmania donovani --- whole-genome sequencing (WGS) --- transcriptome --- artemisinin drug resistance --- Leishmania viruses --- phylogeny --- coevolution --- endosymbiont protozoan viruses --- transcriptome assembly --- transcriptional regulation --- ontology network --- co-expression network --- taxonomic analysis --- database contamination --- kleptoplastidy

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Biotechnology --- Genetic engineering --- Biochemical engineering --- Biomedical Engineering. --- Environmental Microbiology. --- Genetic Engineering. --- Microbiological Techniques. --- Technology. --- Génie biochimique --- Biotechnologie. --- Génie génétique. --- Annuaires. --- Biochemical engineering. --- Biotechnology. --- Genetic engineering. --- Periodicals. --- Arts, Industrial --- Industrial Arts --- Microbiologic Technic --- Microbiologic Technics --- Microbiologic Technique --- Microbiological Technics --- Technic, Microbiologic --- Technics, Microbiological --- Technique, Microbiologic --- Techniques, Microbiologic --- Microbiologic Techniques --- Microbiological Technic --- Microbiological Technique --- Technic, Microbiological --- Technics, Microbiologic --- Technique, Microbiological --- Techniques, Microbiological --- Microbiology --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Microbiology, Environmental --- Environment --- Engineering, Biomedical --- Clinical Engineering --- Engineering, Clinical --- Designed genetic change --- Gene splicing --- Genetic intervention --- Genetic surgery --- Bio-process engineering --- Bioprocess engineering --- methods --- microbiology --- Cloud Computing --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Biomedical Technology --- Genetic recombination --- Transgenic organisms --- Chemical engineering --- Biochemistry --- Génie biochimique --- Génie génétique. --- genetic manipulation --- genetic modification --- Breeding and Genetic Improvement --- molecular genetics --- antibody engineering --- cisgenesis --- directed evolution --- DNA shuffling --- gene editing --- genetically engineered traits --- intragenesis --- nuclear transplantation --- paratransgenesis --- protein engineering --- sex preselection --- transgenesis --- bioengineering --- bioethics --- biopharmaceuticals --- biotechnology --- food biotechnology --- gene therapy --- genetic improvement --- genetic techniques and protocols --- genetically modified foods --- genetically modified organisms --- genetically modified plants --- metabolic engineering --- molecular farming --- reverse genetics --- Serials --- Biomedical engineering. --- Microbial ecology. --- Biomedical Engineering --- Environmental Microbiology --- Genetic Engineering --- Microbiological Techniques --- Technology --- Biotechnologie --- Génie biomédical. --- Écologie microbienne. --- Technologie. --- biomedical engineering. --- Environmental microbiology --- Microorganisms --- Ecology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Applied science --- Arts, Useful --- Science, Applied --- Useful arts --- Science --- Industrial arts --- Material culture --- genetic engineering