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Molecular biology --- DNA Replication --- DNA --- Biosynthesis --- DNA replication --- biosynthesis --- DNA Replication. --- 577.213.3 --- 577.21 --- Biochemie : DNA --- DNA-replicatie --- Chromosomal DNA replication --- Replication of DNA --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- biosynthesis. --- Enzymatic synthesis in vitro of DNA, of RNA. DNA-polymerase, RNA-replicase systems --- Replication --- Synthesis --- DNA replication. --- ADN --- Réplication --- 577.213.3 Enzymatic synthesis in vitro of DNA, of RNA. DNA-polymerase, RNA-replicase systems --- Réplication --- DNA - biosynthesis --- ENZYMES --- GENES --- MOLECULAR STRUCTURE --- VIRUS --- STRUCTURES
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DNA --- Synthesis --- Synthesis. --- DNA Replication --- DNA replication --- Réplication --- DNA Replication. --- 577.213.3 --- Chromosomal DNA replication --- Replication of DNA --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- biosynthesis. --- Enzymatic synthesis in vitro of DNA, of RNA. DNA-polymerase, RNA-replicase systems --- Replication --- Nucleic Acids --- Nucleic Acids. --- 577.213.3 Enzymatic synthesis in vitro of DNA, of RNA. DNA-polymerase, RNA-replicase systems --- Réplication --- Deoxyribonucleic acid synthesis --- biosynthesis --- Molecular biology --- Basic Sciences. Molecular Biology --- ADN --- Synthèse --- DNA - Synthesis. --- DNA Repair. --- DNA - Synthesis --- DNA Repair --- Deoxyribonucleoproteins --- Enzymes --- Nucleotidyltransferase, deoxyribonucleate --- Plasmids --- Recombination, genetic
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Virus Replication. --- DNA Replication. --- Neoplasms. --- Oncogenic viruses --- -Viral genetics --- -Viruses --- -Genetic vectors --- Microorganisms --- Mobile genetic elements --- Extrachromosomal DNA --- Virus genetics --- Viruses --- Microbial genetics --- Cancer-causing viruses --- Cancer viruses --- Oncoviruses (Oncogenic viruses) --- Tumor viruses --- Tumorigenic viruses --- Viral carcinogenesis --- Benign Neoplasms --- Neoplasia --- Neoplasm --- Neoplasms, Benign --- Cancer --- Tumors --- Benign Neoplasm --- Cancers --- Neoplasm, Benign --- Tumor --- Medical Oncology --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Replication, Virus --- Replications, Virus --- Virus Replications --- DNA Replication --- Congresses --- Reproduction --- -Congresses --- Genetics --- Viral genetics --- DNA replication --- NEOPLASMS --- VIRUS REPLICATION --- Congresses. --- Malignancy --- Malignancies --- Neoplasias --- Dna replication --- Neoplasms --- Virus replication --- -Benign Neoplasms --- Malignant Neoplasms --- Malignant Neoplasm --- Neoplasm, Malignant --- Neoplasms, Malignant --- Genetic vectors --- Reproduction&delete& --- Viral Replication --- Replication, Viral --- Replications, Viral --- Viral Replications --- Viral Replication Compartments
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Genetic Engineering --- Recombination, Genetic --- DNA Replication --- Neoplasms --- Oncogenic viruses --- Genetic engineering --- Cancer --- Virus cancérogènes --- Génie génétique --- Genetic aspects --- Aspect génétique --- DNA, Recombinant. --- Genetic Engineering. --- Neoplasms. --- Recombination, Genetic. --- DNA Replication. --- -Genetic engineering --- -Oncogenic viruses --- -Cancer-causing viruses --- Cancer viruses --- Oncoviruses (Oncogenic viruses) --- Tumor viruses --- Tumorigenic viruses --- Viruses --- Viral carcinogenesis --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Recombination --- Genetic Recombination --- Genetic Recombinations --- Recombinations --- Recombinations, Genetic --- Benign Neoplasms --- Neoplasia --- Neoplasm --- Neoplasms, Benign --- Benign Neoplasm --- Neoplasm, Benign --- Tumor --- Medical Oncology --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- Recombinant DNA --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- DNA Transposable Elements --- Genetic Vectors --- Genomic Library --- Gene Library --- -Congresses --- Congresses --- DNA replication --- Congresses. --- Malignancy --- Malignancies --- Neoplasias --- -Genetic aspects --- Dna replication --- Recombination, genetic --- Virus cancérogènes --- Génie génétique --- Aspect génétique --- Malignant Neoplasms --- Malignant Neoplasm --- Neoplasm, Malignant --- Neoplasms, Malignant --- Genetic aspects&delete&
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PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis. The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls. This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.
General biochemistry --- DNA (deoxyribonucleic acid) --- biochemie --- PCR (polymerase chain reaction) --- genetische informatie --- Molecular biology --- Analytical biochemistry --- biologie --- moleculaire biologie --- polymeren --- Macromolecules --- Gene Amplification --- Réaction en chaîne de la polymérase --- Technique des traceurs --- Tracer techniques --- État physique --- physical states --- Polymerase Chain Reaction. --- -Chain reaction, Polymerase --- PCR (Biochemistry) --- DNA polymerases --- Amplification, Gene --- Anchored Polymerase Chain Reaction --- Inverse Polymerase Chain Reaction --- Nested Polymerase Chain Reaction --- Anchored PCR --- Inverse PCR --- Nested PCR --- PCR, Anchored --- PCR, Inverse --- PCR, Nested --- Polymerase Chain Reactions --- Reaction, Polymerase Chain --- Reactions, Polymerase Chain --- Ligase Chain Reaction --- -Molecular bases of life. Molecular biology --- -577.2 Molecular bases of life. Molecular biology --- Chain reaction, Polymerase --- Réaction en chaîne de la polymérase --- Polymerase chain reaction --- DNA Replication --- Polymerase Chain Reaction --- 577.2 --- 577.2 Molecular bases of life. Molecular biology --- Molecular bases of life. Molecular biology --- PCR --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Laboratory manuals --- #WSCH:WBIO --- Polymerization --- methods --- Laboratory manuals. --- Polymerase chain reaction. --- Dna replication. --- Gene amplification. --- DNA Replication. --- Gene Amplification. --- klonen --- enzymen --- Polymerase chain reaction - Laboratory manuals. --- Genes
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The suggestion for this collection of essays originated in part from a course given to graduate students at the University of Pennsylvania School of Medicine. In sections of this course, the conceptual developments in the fields of membrane transport and cellular respiration were traced to illustrate general aspects of the development of ideas in a scientific field. Discussions with peers on the topic also greatly enhanced the development of the project as it is reflected in this book. The volume reflects the breadth and scope of this rapidly developing field, and is an excellent treatise of a
Cell membranes --- Cell membranes. --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Amino acids --- Biological transport, active. --- Biochemistry --- Biochemistry. --- Biophysics. --- Dna repair. --- Dna replication. --- Dna --- Energy transfer. --- Energy metabolism --- Genetic code. --- Proteins --- Rna --- Sulfur --- Metabolism --- Disorders. --- Metabolism. --- History. --- Biosynthesis. --- Research --- 577.1 --- Biochimie --- History --- Histoire --- DNA --- DNA Repair. --- DNA Replication. --- Genetic Code. --- Protein Biosynthesis. --- RNA --- biosynthesis. --- Molecular biology --- General biochemistry --- History of natural sciences --- Philosophy --- Physics, chemistry, biology. --- General. --- history --- Vitamins --- Trace elements --- Accessory elements --- Microelements --- Micronutrients --- Minerals, Trace --- Minor elements --- Trace metals --- Trace minerals --- Agricultural chemicals --- Chemical elements --- Nutrition --- Avitaminosis --- DNA Repair --- DNA Replication --- Genetic Code --- Protein Biosynthesis --- Protein Biosynthesis, Ribosomal --- Protein Synthesis, Ribosomal --- Ribosomal Peptide Biosynthesis --- mRNA Translation --- Genetic Translation --- Peptide Biosynthesis, Ribosomal --- Protein Translation --- Translation, Genetic --- Biosynthesis, Protein --- Biosynthesis, Ribosomal Peptide --- Biosynthesis, Ribosomal Protein --- Genetic Translations --- Ribosomal Protein Biosynthesis --- Ribosomal Protein Synthesis --- Synthesis, Ribosomal Protein --- Translation, Protein --- Translation, mRNA --- mRNA Translations --- Peptide Biosynthesis --- Codon, Initiator --- Codon, Terminator --- Code, Genetic --- Codes, Genetic --- Genetic Codes --- Codon --- Histone Code --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Base Excision Repair --- Excision Repair --- Nucleotide Excision Repair --- Base Excision Repairs --- Excision Repair, Base --- Excision Repair, Nucleotide --- Excision Repairs --- Nucleotide Excision Repairs --- Repair, Base Excision --- Repair, Excision --- Repairs, Base Excision --- DNA-Formamidopyrimidine Glycosylase --- DNA Repair Enzymes --- Targeted Gene Repair --- biosynthesis --- Issue --- 577.122 --- 577.122 Protein metabolism --- Protein metabolism --- metabolism --- Amino Acid Metabolism, Inborn Errors --- Amino Acids --- 577.1 <035> --- Metabolism&delete& --- Disorders --- Amino Acid Metabolism Disorders, Inborn --- Amino Acid Metabolism, Inborn Error --- Amino Acid Metabolism, Inherited Disorders --- Amino Acidopathies, Inborn --- Congenital Amino Acidopathies --- Inborn Errors, Amino Acid Metabolism --- Inherited Errors of Amino Acid Metabolism --- Amino Acidopathies, Congenital --- Amino Acidopathy, Congenital --- Amino Acidopathy, Inborn --- Congenital Amino Acidopathy --- Inborn Amino Acidopathies --- Inborn Amino Acidopathy --- Amino compounds --- Organic acids --- Peptides --- 577.1 <035> Chemical bases of life. Biochemistry and bio-organic chemistry generally--Grote handboeken. Compendia --- Chemical bases of life. Biochemistry and bio-organic chemistry generally--Grote handboeken. Compendia --- Amino acid metabolism --- Amino acid metabolism, Inborn errors of --- Aminoacidopathies --- Molécules --- Molécules --- Sulphur - Metabolism --- Amino acids - Metabolism - Disorders --- Amino acids - Metabolism --- Amino Acids - metabolism --- Sulfur - metabolism --- Acides aminés --- metabolism. --- Métabolisme --- Bioenergetics --- Microbial respiration --- Carbohydrates --- Carbohydrate metabolism --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Biochemistry - history --- Energy metabolism - Research - History --- Structure moléculaire --- Alchemy [not cas]
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