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This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. .
Recombinació genètica --- Cromosomes --- Enginyeria genètica --- ADN recombinant --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects
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Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Genetics. --- Genetics and Genomics. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Enginyeria genètica --- Regulació genètica --- Genomes --- Genoma --- Genoma humà --- Genòmica --- Biosíntesi --- Genètica molecular --- Inducció enzimàtica --- Expressió gènica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
Genetics. --- Genomics. --- Genetics and Genomics. --- Genome research --- Genomes --- Molecular genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Research --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics
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"Encompasses all aspects of chromosome biology and the application of molecular cytogenetic techniques in all areas of biomedicine."
Cytogenetics --- Chromosomes --- Molecular Biology --- Molecular genetics --- Cytogénétique --- Génétique moléculaire --- Periodicals. --- Périodiques --- Cytogenetics. --- Chromosomes. --- Biochemical Phenomena. --- Molecular genetics. --- Chromosome theory --- Biochemical Concepts --- Biochemical Phenomenon --- Biochemical Process --- Phenomena, Biochemical --- Biochemical Processes --- Biochemical Concept --- Concept, Biochemical --- Concepts, Biochemical --- Phenomenon, Biochemical --- Process, Biochemical --- Processes, Biochemical --- Chromosome --- Cytogenetic --- chromosome biology --- medical genetics --- Genetics --- Molecular biology --- Cytology --- Cell nuclei --- Crossing over (Genetics) --- Cytotaxonomy --- Karyokinesis --- Linkage (Genetics) --- Cytogenetic Analysis --- Chromosome Disorders --- Sex Chromosome Disorders --- moleculaire biologie --- Citogenètica. --- Cromosomes. --- Genètica molecular. --- Biologia molecular. --- Biofísica molecular --- Bioquímica molecular --- Biofísica --- Bioquímica --- Histoquímica --- Biologia molecular vegetal --- Codi genètic --- Diagnòstic molecular --- Endocrinologia molecular --- Evolució molecular --- Farmacologia molecular --- Genètica molecular --- Glicòmica --- Metabolòmica --- Microbiologia molecular --- Neurobiologia molecular --- Patologia molecular --- Proteòmica --- Reconeixement molecular --- Biomolècules --- Biologia molecular --- Genètica --- Clonatge molecular --- Elements genètics mòbils --- Genètica bioquímica --- Genètica molecular humana --- Genètica molecular vegetal --- Genòmica --- Gens --- Regulació genètica --- Variació en el nombre de còpies --- Herència (Biologia) --- Nuclis cel·lulars --- Anomalies cromosòmiques --- Al·lelomorfisme --- Cariotips --- Cromatina --- Cromosomes humans --- Cromosomes sexuals --- Recombinació genètica --- Telòmer --- Translocació (Genètica) --- Genètica cel·lular --- Citologia --- Citogenètica humana --- Mapatge cromosòmic
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This updated second edition covers the molecular biology, genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum. Aside from modern omics-based approaches, the authors also focus on cell physiology, including cell division, central carbon metabolic pathways, and the respiratory chain. Readers will learn how primary mechanisms like energy metabolism can be applied in processes like biorefinery. Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. These chapters will be particularly useful for those interested in the microbial production of commodity chemicals, fuels, and proteins. Corynebacteriacea are already some of the most important industrial microorganisms. Understanding the cell physiology of C. glutamicum will help manufacturers to increase their product range and productivity through efficient metabolic engineering.
Microbiology. --- Biochemistry. --- Applied Microbiology. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Microbial biology --- Microorganisms --- Composition --- Corynebacterium glutamicum. --- Corynebacterium lilium --- Micrococcus glutamicus --- Corynebacterium --- Corynebacterium glutamicum --- Bacteris --- Enginyeria genètica --- Biologia molecular --- Biofísica molecular --- Bioquímica molecular --- Biofísica --- Bioquímica --- Histoquímica --- Biologia molecular vegetal --- Codi genètic --- Diagnòstic molecular --- Endocrinologia molecular --- Evolució molecular --- Farmacologia molecular --- Genètica molecular --- Glicòmica --- Metabolòmica --- Microbiologia molecular --- Neurobiologia molecular --- Patologia molecular --- Proteòmica --- Reconeixement molecular --- Biomolècules --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Microbis --- Microorganismes --- Antígens bacterians --- Bacteris anaerobis --- Bacteris fotosintetitzadors --- Bacteris gramnegatius --- Bacteris nitrificants --- Bacteris patògens --- Lactobacils --- Listeria --- Virus
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This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules. Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and sustainable manner by extending or modifying biosynthetic pathways in a wide range of organisms. It has been successfully used to produce chemicals, drugs, enzymes, amino acids, antibiotics, biofuels, and industrially important pharmaceuticals. The book comprehensively reviews the various metabolites detection, extraction and biosensors and the metabolic engineering of microbial strains for the production of industrially useful enzymes, proteins, organic acids, vitamins and antibiotics, therapeutics, chemicals, and biofuels. It also discusses various genetic engineering and synthetic biology tools for metabolic engineering. In closing, the book discusses ethical, patenting and regulatory issues in the metabolic engineering of microbes. This book is a valuable source not only for beginners in metabolic engineering, but also students, researchers, biotechnology and metabolic engineering based company.
Biomedical engineering. --- Genetic engineering. --- Microbiology. --- Biochemical engineering. --- Bioethics. --- Computer simulation. --- Biomedical Engineering/Biotechnology. --- Genetic Engineering. --- Biochemical Engineering. --- Simulation and Modeling. --- Computer modeling --- Computer models --- Modeling, Computer --- Models, Computer --- Simulation, Computer --- Electromechanical analogies --- Mathematical models --- Simulation methods --- Model-integrated computing --- Biology --- Biomedical ethics --- Life sciences --- Life sciences ethics --- Science --- Bio-process engineering --- Bioprocess engineering --- Biochemistry --- Biotechnology --- Chemical engineering --- Microbial biology --- Microorganisms --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Moral and ethical aspects --- Enginyeria biomèdica --- Enginyeria genètica --- Microbiologia --- Biologia --- Bacteriologia --- Ecologia microbiana --- Genètica microbiana --- Microbiologia agrícola --- Microbiologia aquàtica --- Microbiologia dels sòls --- Microbiologia mèdica --- Microbiologia molecular --- Protozous --- Química microbiològica --- Virologia --- Microorganismes --- Microscòpia --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Enginyeria clínica --- Enginyeria mèdica --- Bioenginyeria --- Biofísica --- Enginyeria --- Medicina --- Electrònica mèdica --- Enginyeria de teixits --- Materials biomèdics --- Aparells i instruments mèdics
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This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.
Arròs --- Genètica vegetal --- Enginyeria genètica --- Millorament selectiu de plantes --- Millora de plantes --- Millorament de conreus --- Millorament genètic de conreus --- Millorament genètic de les plantes --- Millorament genètic de plantes conreades --- Millorament vegetal --- Selecció artificial de les plantes --- Agricultura --- Millora de les espècies --- Hibridació vegetal --- Immunitat de les plantes --- Plantes transgèniques --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Oryza sativa --- Cereals --- Cuina (Arròs) --- Botany. --- Food—Microbiology. --- Genetics. --- Plant Science. --- Food Microbiology. --- Genetics and Genomics. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Natural history --- Plants
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Systems and Synthetic Immunology focuses on the similarities between biology and engineering at the systems level, which are important for applying engineering theories to biology problems. With the advent of new genomic techniques, there are numerous systematic investigations underway in the scientific world. This volume highlights techniques that can be used to effectively combine two of the most essential biological fields - Systems Biology and Synthetic Immunology. The respective chapters discuss the role of synthetic immunology in biotechnology, production of biomaterials, and their use in vaccine delivery. Further topics include the importance of cytokines; the use of genomic engineering tools in immunotherapy; immunosensors; nanotherapeutics; and bioinformatics tools in biomedical applications. Given its scope, the book offers readers an up-to-date and comprehensive review of this unique and dynamic field of research.
Immunology. --- Systems biology. --- Computational biology --- Bioinformatics --- Biological systems --- Molecular biology --- Immunobiology --- Life sciences --- Serology --- Biomedical engineering. --- Cytokines. --- Growth factors. --- Genetic engineering. --- Systems Biology. --- Biomedical Engineering/Biotechnology. --- Cytokines and Growth Factors. --- Genetic Engineering. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cell growth factors --- Cellular growth factors --- Growth peptides --- Growth promoting substances --- Growth substances --- Peptide growth factors --- Peptide regulatory factors --- Polypeptide growth factors --- Cytokines --- Peptides --- Cellular immunity --- Immune response --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Regulation --- Sistema immunitari --- Biologia --- Enginyeria genètica --- Intervenció genètica --- Manipulació genètica --- Recombinació genètica --- ADN recombinant --- Clonatge --- Clonatge molecular --- Enginyeria de proteïnes --- Enginyeria genètica animal --- Enginyeria genètica vegetal --- Teràpia genètica --- Fecundació in vitro --- Organismes transgènics --- Ciències biològiques --- Ciències de la vida --- Adaptació (Biologia) --- Anatomia --- Biodiversitat --- Bioelectrònica --- Bioenginyeria --- Bioespeleologia --- Biofísica --- Biogeografia --- Biolingüística --- Biologia aquàtica --- Biologia computacional --- Biologia de la conservació --- Biologia de poblacions --- Biologia del desenvolupament --- Biologia econòmica --- Biologia espacial --- Biologia experimental --- Biologia humana --- Biomatemàtica --- Biometria --- Bioquímica --- Biotecnologia --- Cèl·lules --- Citologia --- Criobiologia --- Cronobiologia --- Ecologia --- Embriologia --- Evolució (Biologia) --- Exobiologia --- Fecundació --- Filogènia --- Fisiologia --- Fotobiologia --- Generació espontània --- Genètica --- Geobiologia --- Immunologia --- Metabolisme --- Microbiologia --- Mimetisme (Biologia) --- Morfogènesi --- Mort (Biologia) --- Neurobiologia --- Ontogènesi --- Parasitologia --- Partenogènesi --- Protoplasma --- Psicobiologia --- Radiobiologia --- Regeneració (Biologia) --- Reproducció --- Sexualitat (Biologia) --- Simbiosi --- Variació (Biologia) --- Vitalisme --- Biomassa --- Ciències naturals --- Evolució --- Història de la biologia --- Il·lustració biològica --- Vida (Biologia) --- Sistema immunològic --- Cèl·lules immunocompetents --- Medul·la òssia --- Sistema limfàtic
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