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Maintaining the high standards set by the successful first edition, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical industry and academia take advantage of the latest developments to provide detailed practical methods for studying receptors in silico, in vitro, and in vivo. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knock-out mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan polymerase chain reaction (PCR). These ligand binding techniques are perfect for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Receptor Binding Techniques, Second Edition offers academic and commercial researchers in the pharmaceutical and biotechnology industries a set of proven techniques for the successful characterization of receptors and the phenotyping of transgenic animals, including knock-outs.

Pharmacy. --- Chemistry --- Medicine --- Drugs --- Materia medica --- Pharmacology --- Receptor-ligand complexes --- Radioligand assay --- Cell receptors --- Research

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Ligands --- Radioligand Assay --- Signal Transduction --- Cell receptors --- Drug receptors --- Ligand binding (Biochemistry) --- Radioligand assay --- Receptor-ligand complexes --- Ligands (Biochimie) --- Complexes récepteur-ligand --- methods --- Research --- Methodology --- Fixation --- Radioligand assay. --- Receptor-ligand complexes. --- Methodology. --- Ligand binding (Biochemistry). --- Complexes récepteur-ligand --- Ligands. --- Signal Transduction. --- methods.

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In Receptor Binding Techniques, detailed experimental protocols devised by expert practitioners demonstrate the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein-coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also take into account the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations. Receptor Binding Techniques shows clearly how these techniques can be adapted to many different applications and fully utilized in drug discovery programs. It will be of high value not only to researchers new to the field of receptor binding, but also to more experienced workers, serving as an authoritative bench manual replete with optimalized practical methods and protocols. Pharmacologists, neuroscientists, cell biologists, molecular biologists, and biochemists.

Receptor-ligand complexes --- Radioligand assay --- Cell receptors --- Radioligand Assay --- Methods --- Receptors, Drug --- Binding Sites --- Diagnostic Techniques, Endocrine --- Investigative Techniques --- Laboratory Techniques and Procedures --- Molecular Structure --- Diagnostic Techniques, Radioisotope --- Proteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Diagnostic Techniques and Procedures --- Amino Acids, Peptides, and Proteins --- Biochemical Phenomena --- Diagnosis --- Chemical Phenomena --- Chemicals and Drugs --- Phenomena and Processes --- Cytology --- Biology --- Health & Biological Sciences --- Laboaratory manuals --- Research --- Laboratory manuals --- Binding Sites. --- Laboratory manuals. --- Laboaratory manuals. --- methods. --- isolation & purification. --- Methods. --- Isolation & purification. --- Medicine. --- Pharmacology. --- Biomedicine. --- Pharmacology/Toxicology. --- Drug effects --- Medical pharmacology --- Medical sciences --- Chemicals --- Chemotherapy --- Drugs --- Pharmacy --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Physiological effect --- Toxicology. --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Toxicology

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Nuclear Receptors are inducible transcription factors that mediate complex effects on development, differentiation and homeostasis. They regulate the transcription of their target genes through binding to DNA sequences.*Analysis of Nuclear Receptor Ligands*Structure/Function Analysis of Nuclear Receptors*Analysis of Nuclear Receptor Co-Factors and Chromatin Remodeling

Nuclear receptors (Biochemistry). --- Laboratory Chemicals --- Proteins --- DNA-Binding Proteins --- Investigative Techniques --- Transcription Factors --- Biochemical Phenomena --- Pharmacological Phenomena --- Physiological Phenomena --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Amino Acids, Peptides, and Proteins --- Specialty Uses of Chemicals --- Chemical Phenomena --- Phenomena and Processes --- Chemical Actions and Uses --- Chemicals and Drugs --- Receptors, Cytoplasmic and Nuclear --- Structure-Activity Relationship --- Models, Animal --- Ligands --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Experimental Animal Models --- Laboratory Animal Models --- Animal Model --- Animal Model, Experimental --- Animal Model, Laboratory --- Animal Models --- Animal Models, Experimental --- Animal Models, Laboratory --- Experimental Animal Model --- Laboratory Animal Model --- Model, Animal --- Model, Experimental Animal --- Model, Laboratory Animal --- Models, Experimental Animal --- Models, Laboratory Animal --- Animal Experimentation --- Relationship, Structure-Activity --- Relationships, Structure-Activity --- Structure Activity Relationship --- Structure-Activity Relationships --- Cytoplasmic Hormone Receptors --- Cytoplasmic Receptors --- Cytosol and Nuclear Receptors --- Intracellular Membrane Receptors --- Nuclear Hormone Receptors --- Nuclear Receptors --- Receptors, Cytoplasmic --- Receptors, Cytosol and Nuclear --- Receptors, Cytosolic and Nuclear --- Receptors, Intracellular Membrane --- Receptors, Nuclear --- Receptors, Nuclear and Cytoplasmic --- Cytoplasmic and Nuclear Receptors --- Cytosolic and Nuclear Receptors --- Hormone Receptors, Cytoplasmic --- Hormone Receptors, Nuclear --- Nuclear and Cytoplasmic Receptors --- Membrane Receptors, Intracellular --- Receptors, Cytoplasmic Hormone --- Receptors, Nuclear Hormone --- Hormones --- Chemical Phenomenon --- Chemical Process --- Physical Chemistry Phenomena --- Physical Chemistry Process --- Physicochemical Phenomenon --- Physicochemical Process --- Chemical Concepts --- Chemical Processes --- Physical Chemistry Concepts --- Physical Chemistry Processes --- Physicochemical Concepts --- Physicochemical Phenomena --- Physicochemical Processes --- Chemical Concept --- Chemistry Process, Physical --- Chemistry Processes, Physical --- Concept, Chemical --- Concept, Physical Chemistry --- Concept, Physicochemical --- Concepts, Chemical --- Concepts, Physical Chemistry --- Concepts, Physicochemical --- Phenomena, Chemical --- Phenomena, Physical Chemistry --- Phenomena, Physicochemical --- Phenomenon, Chemical --- Phenomenon, Physicochemical --- Physical Chemistry Concept --- Physicochemical Concept --- Process, Chemical --- Process, Physical Chemistry --- Process, Physicochemical --- Processes, Chemical --- Processes, Physical Chemistry --- Processes, Physicochemical --- Specialty Chemicals and Products --- Physiological Concepts --- Physiological Phenomenon --- Physiological Process --- Physiological Processes --- Concept, Physiological --- Concepts, Physiological --- Phenomena, Physiological --- Phenomenas, Physiological --- Phenomenon, Physiological --- Physiological Concept --- Process, Physiological --- Processes, Physiological --- Pharmacologic Phenomena --- Pharmacologic Phenomenon --- Pharmacologic Process --- Pharmacological Concepts --- Pharmacological Phenomenon --- Pharmacologic Processes --- Pharmacological Processes --- Concept, Pharmacological --- Concepts, Pharmacological --- Pharmacological Concept --- Phenomena, Pharmacologic --- Phenomena, Pharmacological --- Phenomenon, Pharmacologic --- Phenomenon, Pharmacological --- Process, Pharmacologic --- Processes, Pharmacologic --- Processes, Pharmacological --- Biochemical Concepts --- Biochemical Phenomenon --- Biochemical Process --- Phenomena, Biochemical --- Biochemical Processes --- Biochemical Concept --- Concept, Biochemical --- Concepts, Biochemical --- Phenomenon, Biochemical --- Process, Biochemical --- Processes, Biochemical --- Molecular Biology --- Transcription Factor --- Factor, Transcription --- Factors, Transcription --- Gene Expression Regulation --- Transcription, Genetic --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- DNA Binding Protein --- DNA Single-Stranded Binding Protein --- SS DNA BP --- Single-Stranded DNA-Binding Protein --- DNA Helix Destabilizing Proteins --- Single-Stranded DNA Binding Proteins --- Binding Protein, DNA --- DNA Binding Proteins --- DNA Single Stranded Binding Protein --- DNA-Binding Protein, Single-Stranded --- Single Stranded DNA Binding Protein --- Single Stranded DNA Binding Proteins --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- Chemicals, Laboratory --- Ligand --- Cytoplasmic Receptor --- Nuclear Hormone Receptor --- Nuclear Receptor --- Hormone Receptor, Nuclear --- Receptor, Cytoplasmic --- Receptor, Nuclear --- Receptor, Nuclear Hormone --- DNA-Binding Protein --- Protein, DNA-Binding --- Protein --- Hormone receptors. --- Nuclear receptors (Biochemistry) --- Receptor-ligand complexes.