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Ribozyme Protocols brings together a broad spectrum of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA molecules and to inactivate gene expression in vivo. The methods collected here range from theoretical design to clinical application and include the synthesis and assay of ribozymes, as well as their use both in vitro and in vivo. Each method is presented by an expert with substantial hands-on experience and in far greater detail than is generally to be found in the research literature. Ribozyme Protocols will help all investigators perform meaningful and successful experiments using ribozymes in both clinical and basic research, as well as launch fresh application initiatives. It is certain to be found indispensable not only by those workers new to the field, but also by experienced researchers who wish to expand their range of expertise.

Catalytic RNA -- Laboratory manuals. --- Electronic books. -- local. --- Nucleic acids -- Laboratory manuals. --- Catalytic RNA --- Genetic Techniques --- RNA, Catalytic --- RNA, Untranslated --- Investigative Techniques --- Enzymes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Enzymes and Coenzymes --- RNA --- Chemicals and Drugs --- Nucleic Acids --- Nucleic Acids, Nucleotides, and Nucleosides --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals --- RNA, Catalytic. --- Genetic Techniques. --- Laboratory manuals. --- Catalytic rna --- Genetic techniques. --- Rna, catalytic. --- Nucleic acids --- Polynucleotides --- Biomolecules --- Ribonucleic acid enzymes --- Ribozymes --- RNA enzymes --- Non-coding RNA --- Nucleases --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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In this book, seven chapters describe studies aimed at understanding and exploiting the key features of such molecular RNA and DNA devices. In the first section of the book, four chapters are devoted to artificial nucleic acid switches and sensors. These chapters introduce the concept of allosteric ribozymes as molecular switches and sensors; describe nucleic acid enzymes that are switched by oligonucleotides and other nucleic acid enzymes that are switched by proteins; and illustrate how switching elements can be integrated ration-ally into fluorescently signaling molecular sensors made out of nucleic acids. In the second section of the book, three chapters show that nature has been as crafty a molecular-scale engineer as any modern scientist via evolution of natural nucleic acid switches and sensors. RNAs have been found whose activities are modulated either by proteins or by small-molecule metabolites, and both kinds of system are described. Finally, the notion of exploiting naturally occurring RNA switches for drug development is discussed.

Biomedicine. --- Human Genetics. --- Medical Microbiology. --- Medicine. --- Human genetics. --- Microbiology. --- Médecine --- Génétique humaine --- Microbiologie --- Biosensors. --- Catalytic RNA. --- RNA -- Biotechnology. --- Catalytic RNA --- Biosensors --- RNA --- Enzymes --- Nucleic Acids, Nucleotides, and Nucleosides --- RNA, Untranslated --- Genes, Regulator --- Molecular Probe Techniques --- Metabolic Phenomena --- RNA, Catalytic --- Biosensing Techniques --- Metabolism --- Genes, Switch --- Nucleic Acids --- Investigative Techniques --- Genes --- Chemicals and Drugs --- Enzymes and Coenzymes --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genome Components --- Genome --- Genetic Structures --- Genetic Phenomena --- Human Anatomy & Physiology --- Biology --- Medicine --- Pathology --- Animal Biochemistry --- Genetics --- Health & Biological Sciences --- Biotechnology --- Biotechnology. --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Ribonucleic acid enzymes --- Ribozymes --- RNA enzymes --- Medical microbiology. --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Non-coding RNA --- Nucleases