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Dissertation
Thesis, COLLÉGIALITÉ
Authors: --- --- --- ---
Year: 2022 Publisher: Liège Université de Liège (ULiège)

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Lymphangiogenesis consists in the formation of new lymphatic vessels from already&#13;established ones. This complex biological process is triggered during inflammation, wound repair or cancer. A decrease or defect in lymphangiogenesis leads to a pathology called lymphedema. This condition is characterised by tissue swelling caused by the accumulation of interstitial fluids. Nowadays there is no curative therapy. Vascular Endothelial Growth Factor Receptors 2 and 3 (VEGFR 2/3) represent interesting therapeutic targets as they are known to be major lymphangiogenesis inducers. Previous work from the host laboratory has identified the endocytic receptor “urokinase plasminogen activator receptor-associated protein” (“uPARAP” encoded by MRC2 gene) as negatively regulating lymphangiogenesis. uPARAP interacts with VEGFR 2/3 and blocks their heterodimerisation and signalisation. More recently, the laboratory has identified the interaction of uPARAP with Vascular Endothelial cadherin (VE-cadherin), a transmembrane protein also known to play key roles in lymphangiogenesis.&#13;The aim of this work is to identify the binding site between uPARAP and VE-cadherin or VEGFR 2/3 in order to provide better knowledge on uPARAP’s functions in lymphatics.&#13;A collaboration with Pr. Miikka Vikkula (UCL) had previously identified 7 uPARAP variants from a primary lymphedema patient cohort. We generated and validated upon this work multiple engineered A431 cell lines to express some known uPARAP partners (VE- cadherin, VEGF-R2 and VEGF-R3). Through proximity ligation assays, we successfully identified two variants (Thr653Met, Arg680Trp) who decreased uPARAP interactions with VE- cadherin. Finally, we tried but failed to produce new lentiviral vectors containing the previously identified variants. Those vectors were though to permit the investigation of variant impacts in primary human lymphatic endothelial cells Le processus de génération de nouveaux vaisseaux sanguins à partir de vaisseaux&#13;préexistants est appelé ‘’lymphangiogenèse’’. Ce processus biologique complexe est déclenché pendant l’inflammation, lors des processus de réparation des tissus ou boen encore lors de cancers. Une diminution ou un défaut de lymphangiogenèse mène au développement d’une pathologie appelée ‘’lymphoedème’’. Cette pathologie est chartérisée par le gonflement des tissus causé par une accumulation de fluides au sein des tissus. Aucune thérapie n’existe actuellement pour soigner définitivement cette pathologie. Les récepteurs aux facteurs de croissance vasculaire endothélial (vascular endothelial growth factor receptors -VEGFRs-) de type 2 et 3 (VEGF-R2 et VEGF-R3) forment des cibles thérapeutiques intéressantes de par leurs rôles majeurs dans l’induction et la régulation de la lymphangiogenèse. De précédent travaux au sein du laboratoire d’accueil ont identifié le récepteur endocytique ‘’urokinase plasminogen activator receptor-associated protein’’ (uPARAP, produit de l’expression du gene MRC2) comme régulant négativement la lymphangiogenèse. uPARAP inhibe la formation d’hétérodimeres VEGF-R2/VEGF-R3 et bloque ainsi leur signalisation. Plus récemment, le laboratoire d’accueil a identifié l’interaction d’uPARAP avec la vascular endothelial cadherin (VE-cadherin), une protein transmembranaire également connue dans la lymphangiogenèse.&#13;Le but de ce travail est d’identifier le ou les site(s) de liaison d’uPARAP avec ses différents partenaires (VE-cadherin ou VEGF-R2/R3) afin d’obtenir une plus profonde connaissance des fonctions d’uPARAP dans le système lymphatique.&#13;En collaboration avec le Professeur Miikka Vikkula (UCL), 7 variants ont précédemment été identifiés. Au cours de ce travail, nous avons induit et validé l’expression de la VE-cadherin at des VEGF-R2 et R3 dans une lignée de cellules cancéreuses (A431). Nous avons ensuite identifié avec succès deux variants (Thr653Met, Arg680Trp) diminuant l’interaction d’uPARAP avec la VE-cadherin. Enfin, nous avons essayé mais échoué à produire de nouveau vecteurs lentiviraux contenant les différents variants. Ces vecteurs étaient destinés à l’évaluation de l’impact des variants au sein de cellules endothéliales lymphatiques humaines.


Book
Microsoft System Center 2012 service manager cookbook
Authors: --- ---
ISBN: 1283739097 1849686955 9781849686952 9781849686945 1849686947 9781283739092 Year: 2012 Publisher: Birmingham Packt Pub.

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Abstract

This book is written in practical cookbook style and it offers learning through vivid examples and. Each chapter contains step-by-step instructions about everything necessary to execute a particular task. The book is designed so that you can read it from start to end for beginners or just open up any chapter and start following the recipes as a reference for advanced users,This book will be useful to IT professionals including System Center Service Manager administrators who want to configure and administer System Center Service Manager 2012 and understand how to solve specific problems and


Book
Microsoft System Center Configuration Manager Advanced Deployment
Author:
ISBN: 1782172092 9781782172093 1322166080 9781322166087 1782172084 9781782172086 9781782172086 Year: 2014 Publisher: Birmingham Packt Publishing

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If you are an experienced Configuration Manager administrator looking to advance your career or get more from your current environment, then this book is ideal for you. Prior experience of deploying and managing a Configuration Manager site would be helpful in following the examples throughout this book.


Book
Microsoft System center configuration manager
Author:
ISBN: 1782176772 1306157897 9781306157896 9781782176770 Year: 2013 Publisher: Birmingham Packt Publishing

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Microsoft Configuration Manager can promote high reliability and performance for your computers and mobile devices, and this book will give you the skills to deploy it effectively using best practices. Deploy highly available Configuration Manager sites and roles Backup, restore, and copy Configuration Manager to other sites Get to grips with performance tuning and best practices for Configuration Manager sites In Detail Microsoft Configuration Manager is both extensive and complex, and for many, it is the primary tool for Enterprise management. With each new release, Configuration Manager continually proves itself to be the ultimate solution for managing both clients and mobile devices. This book covers in detailed and easy-to-understand steps how to set up highly available Configuration Manager roles and backend services such as SQL, DNS, and AD. You will learn how to plan for high availability, what kind of roles there are, and how they scale. The book starts by examining what needs to be taken into account when planning for high availability before moving on to focus on the different roles and how they can be set up. The book will also go through different scenarios as well as various backup and recovery procedures. You will learn how to identify bottlenecks within the different components and create sample design scenarios for high availability on Configuration Manager. The book will also look at the different high availability options and how to deploy them.


Book
System Center 2016 virtual machine manager cookbook
Authors: ---
ISBN: 1785881973 9781785881978 9781785881480 1785881485 9781785881480 Year: 2018 Publisher: Birmingham, UK

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Microsoft System Center Virtual Machine Manager (SCVMM) focuses on efficiency with multiple features to help administrators consolidate physical servers within a centrally virtualized environment. This book will allow you to implement the Microsoft System Center family of components effectively and efficiently.


Book
Microsoft System Center Virtual Machine Manager 2012 cookbook
Author:
ISBN: 9781849686334 1849686335 1849686327 9781849686327 129944105X Year: 2013 Publisher: Birmingham [England] Packt Publishing Limited

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This is a Packt Cookbook, full with over 75 recipes for VMM users to carry out vital tasks quickly and easily.This book is written for solutions architects, technical consultants, administrators, and any other virtualization lover who needs to use Microsoft System Center Virtual Machine Manager in a real world environment.


Book
System center 2012 R2 virtual machine manager cookbook
Author:
ISBN: 1782176853 9781782176855 1306902754 9781306902755 1782176845 9781782176848 Year: 2014 Publisher: Birmingham Packt Publishing

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This book is a step-by-step guide packed with recipes that cover architecture design and planning. The book is also full of deployment tips, techniques, and solutions. If you are a solutions architect, technical consultant, administrator, or any other virtualization enthusiast who needs to use Microsoft System Center Virtual Machine Manager in a real-world environment, then this is the book for you. We assume that you have previous experience with Windows 2012 R2 and Hyper-V.


Book
Thoughts on designing information
Authors: --- --- --- ---
ISBN: 9783037784365 3037784369 Year: 2014 Publisher: Zürich Lars Müller Publishers

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Sixteen internationally reputed designers were interviewed by the editors Inge Gobert and Johan Van Looveren. All are active in the broad field of information design: interactive, editorial, and environmental design, data visualization, way finding, typography, cartography. . . This book contains reflections on the field of information design and its boundaries, working methods, client-designer relations, attitudes, dreams, and frustrations. Special emphasis is placed on how future information designers can be effectively prepared to work in a world that is supposed to provide constant access to information


Book
GA Houses 52 : Project 1997.
Author:
Year: 1997 Publisher: Tokyo : A.D.A. EDITA Tokyo Co., Ltd.,


Book
Pharmacy Practice and Administration
Author:
Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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The scope of the Special Issue is research and reviews on evaluations of current practice, innovations in medication management, developments in therapeutics, and pharmaceutical science research that informs and improves practice and administration, as well as the social and administrative pharmacy. We will mainly feature original research, reviews, short reports, and clinical studies, but also case reports, descriptive/how-to, and commentary submissions for consideration.

Keywords

Medicine --- Pharmaceutical industries --- older adults --- polypharmacy --- internal medicine ward --- community pharmacy --- drug disposal --- unused medication --- environment --- awareness --- practice --- ambulatory care facilities --- health workforce --- pharmacists --- Taiwan --- Commonwealth Partnerships for Antimicrobial Stewardship (CwPAMS) --- National Health Service (NHS) --- Chief Pharmaceutical Officer’s Global Health Fellowship --- CPhOGH Fellows --- CwPAMS --- pharmacy --- fellowship --- health partnerships --- antimicrobial resistance (AMR) --- global health --- leadership --- non-prescription medicines --- emergency contraception --- community pharmacies --- information gathering --- availability --- pricing --- mystery calls --- ulipristal acetate --- levonorgestrel --- Germany --- e-prescription --- prescription writing --- Jazan --- Saudi Arabia --- prescription errors --- medicine consumption --- defined daily dose --- adolescents and young adults --- cancer patients --- fragility --- pain --- MUR --- medication review --- barriers --- pharmacist --- pregabalin --- abuse --- community pharmacists --- reproductive health --- contraception --- emergency contraceptives --- patient education --- biosensing technology --- digital medicine system --- medication adherence --- medication event monitoring system --- nonadherence --- prescription opioids --- mortality --- standardized mortality ratio --- underlying cause of death --- prescriptions --- community pharmacy services --- emergency prescribing --- pharmacy practice --- pharmacy law and regulation --- COVID-19 --- additional monitoring --- black triangle --- adverse event reporting --- pharmacovigilance --- intervention --- outcomes --- systematic review --- pharmacy education --- decision to study in pharmacy --- selecting pharmacy as a first choice --- pharmacogenomics --- learning activity --- asynchronous learning --- virtual learning --- student survey --- Program of All-Inclusive Care for the Elderly --- adverse drug events --- medication-related problems --- drug-related problems --- medication safety --- Medicare --- Medicaid --- financial management --- pharmacy management --- business --- entrepreneurship --- pharmacy students --- perception --- attitudes --- ability --- interprofessional --- simulation --- acute care --- nursing --- students --- standardized patients --- high fidelity --- SPICE-R2 --- older adults --- polypharmacy --- internal medicine ward --- community pharmacy --- drug disposal --- unused medication --- environment --- awareness --- practice --- ambulatory care facilities --- health workforce --- pharmacists --- Taiwan --- Commonwealth Partnerships for Antimicrobial Stewardship (CwPAMS) --- National Health Service (NHS) --- Chief Pharmaceutical Officer’s Global Health Fellowship --- CPhOGH Fellows --- CwPAMS --- pharmacy --- fellowship --- health partnerships --- antimicrobial resistance (AMR) --- global health --- leadership --- non-prescription medicines --- emergency contraception --- community pharmacies --- information gathering --- availability --- pricing --- mystery calls --- ulipristal acetate --- levonorgestrel --- Germany --- e-prescription --- prescription writing --- Jazan --- Saudi Arabia --- prescription errors --- medicine consumption --- defined daily dose --- adolescents and young adults --- cancer patients --- fragility --- pain --- MUR --- medication review --- barriers --- pharmacist --- pregabalin --- abuse --- community pharmacists --- reproductive health --- contraception --- emergency contraceptives --- patient education --- biosensing technology --- digital medicine system --- medication adherence --- medication event monitoring system --- nonadherence --- prescription opioids --- mortality --- standardized mortality ratio --- underlying cause of death --- prescriptions --- community pharmacy services --- emergency prescribing --- pharmacy practice --- pharmacy law and regulation --- COVID-19 --- additional monitoring --- black triangle --- adverse event reporting --- pharmacovigilance --- intervention --- outcomes --- systematic review --- pharmacy education --- decision to study in pharmacy --- selecting pharmacy as a first choice --- pharmacogenomics --- learning activity --- asynchronous learning --- virtual learning --- student survey --- Program of All-Inclusive Care for the Elderly --- adverse drug events --- medication-related problems --- drug-related problems --- medication safety --- Medicare --- Medicaid --- financial management --- pharmacy management --- business --- entrepreneurship --- pharmacy students --- perception --- attitudes --- ability --- interprofessional --- simulation --- acute care --- nursing --- students --- standardized patients --- high fidelity --- SPICE-R2

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