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Bioconjugates --- Biochemistry --- Proteins --- Peptides --- Nucleic Acids --- Synthesis. --- methods. --- chemistry. --- Basic Sciences. Chemistry --- Organic Chemistry. --- #WDIR:vfm2 --- ELSEVIER-B EPUB-LIV-FT --- Bioconjugate chemistry --- Conjugated biomolecules --- Conjugation biochemistry --- Conjugation chemistry --- Biomolecules --- Metabolic conjugation --- Synthesis --- Methods. --- Chemistry. --- Bioconjugates - Synthesis. --- Biochemistry - methods. --- Proteins - chemistry. --- Peptides - chemistry. --- Nucleic Acids - chemistry.

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Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.

Protein binding --- Ligands --- Proteins --- Chemistry Techniques, Analytical --- Protein Binding --- chemistry --- methods --- Chemistry, Analytic -- Laboratory manuals. --- Protein binding -- Laboratory manuals. --- Laboratory Chemicals --- Specialty Uses of Chemicals --- Binding, Protein --- Chemistry, Analytic --- Biochemistry --- Allosteric proteins --- Radioligand assay --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Protein binding - Laboratory manuals --- Ligands - laboratory manuals --- Proteins - chemistry - Laboratory Manuals --- Chemistry Techniques, Analytical - methods - laboratory manuals --- Protein Binding - Laboratory Manuals

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Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors. Authoritative and cutting-edge, Protein Design: Methods and Applications will be of major interest to protein scientists, biochemists, and all experimentalists selecting the strategy most adaptable for their design problem.

Protein engineering --- Protein Engineering --- Proteins --- Protein Conformation --- Protein Folding --- methods --- chemistry --- Protein engineering. --- Biophysical Processes --- Biophysical Phenomena --- Engineering, Protein --- Protein design --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biochemical engineering --- Genetic engineering --- Design --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- 577.122 --- 577.122 Protein metabolism --- Protein metabolism --- Protein Folding, Globular --- Folding, Globular Protein --- Folding, Protein --- Foldings, Globular Protein --- Foldings, Protein --- Globular Protein Folding --- Globular Protein Foldings --- Protein Foldings --- Protein Foldings, Globular --- Proteostasis --- Protein Multimerization --- Intrinsically Disordered Proteins --- Conformation, Protein --- Conformations, Protein --- Protein Conformations --- Protein Engineering - methods --- Proteins - chemistry

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Molecular and Cellular Enzymology addresses not only experienced enzymologists but also students, teachers and academic and industrial researchers who are confronted with enzymological problems during their fundamental or applied research. In this field there is an urgent need for training in order to meet the requirements of both research and industrial endeavours. This book consists of several levels. Practical aspects and elementary explanations are given for the benefit of non-specialists’ and students’ understanding. In order to facilitate the task of students, two typographies have been adopted. The main text corresponds to basic knowledge, whereas text in a smaller font provides more specialised information. Specialists will also find topics more deeply expounded with the principal bibliographic references cited. The bibliography, however, is not exhaustive; the choice includes general books and review articles as well as some specialised articles. In this book, for the first time, the different molecular and cellular aspects of enzymology are presented together. Until now, there has been no book available in which these different aspects are treated in the same volume. In addition, besides the theoretical developments, this book provides a wealth of practical information for experimentalists.

Cells -- Enzymology. --- Cells. --- Enzymes -- Metabolism. --- Enzymes. --- Molecular biology. --- Proteins -- Chemistry. --- Enzymes --- Enzymology --- Molecular biology --- Chemistry --- Human Anatomy & Physiology --- Physical Sciences & Mathematics --- Health & Biological Sciences --- Animal Biochemistry --- Biochemistry --- Enzymology. --- Cytology. --- Cell biology --- Cellular biology --- Molecular biochemistry --- Molecular biophysics --- Biocatalysts --- Ferments --- Soluble ferments --- Life sciences. --- Physical chemistry. --- Medicinal chemistry. --- Medical biochemistry. --- Life Sciences. --- Physical Chemistry. --- Medical Biochemistry. --- Medicinal Chemistry. --- Biology --- Cells --- Cytologists --- Biophysics --- Biomolecules --- Systems biology --- Catalysts --- Proteins --- Chemistry, Physical organic. --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Composition --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Medical biochemistry --- Pathobiochemistry --- Pathological biochemistry --- Pathology --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry