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Proteins --- Crystallization --- Separation --- Proteins - Separation --- PROTEINS --- CRYSTALLIZATION --- LIGHT SCATTERING --- PROPERTIES
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-Proteins --- -Proteids --- -Technique --- Analytical biochemistry --- Proteins --- Biochemistry --- Separation --- Technique --- Eiwitten. --- Elektroforese. --- Biomolecules --- Polypeptides --- Proteomics --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Proteins - Separation --- Biochemistry - Technique
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Capillary electrophoresis (CE) is one of the premier methods for the rapid analysis of pharmaceutical proteins and peptides, and thus now even more important with the sequencing of the human genome and the subsequent advent of proteomics. In Capillary Electrophoresis of Proteins and Peptides, leading academic scientists and expert laboratorians working in the pharmaceutical industry describe their best CE techniques in step-by-step detail for easy reproduction. The authors present cutting-edge techniques for both capillary coatings and analytical detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Additional chapters discuss CE and capillary isoelectric focusing, combined with electrospray mass spectrometry detection, for performing proteomic studies. Areas of special interest covered include proteomic applications, as well as applications in the field of protein-ligand binding. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Capillary Electrophoresis of Proteins and Peptides will prove essential to both new and experienced investigators carrying out significant protein and peptide studies in biotechnology, drug discovery, and pharmaceutical research and development.
Capillary electrophoresis --- Proteins --- Peptides --- Electrophoresis, Capillary --- Separation --- methods --- analysis --- Capillary electrophoresis -- Laboratory manuals. --- Peptides -- Separation -- Laboratory manuals. --- Proteins -- Separation -- Laboratory manuals. --- Electrophoresis --- Amino Acids, Peptides, and Proteins --- Investigative Techniques --- Chemistry Techniques, Analytical --- Chemicals and Drugs --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Electrochemical Techniques --- Methods --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Organic compounds --- Amino acids --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Capillary electrophoresis - Laboratory manuals --- Proteins - Separation - Laboratory manuals --- Peptides - Separation - Laboratory manuals --- Electrophoresis, Capillary - methods --- Proteins - analysis
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All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.
Proteins --- Separation --- Handbooks, manuals, etc --- Amino acids --- Peptides --- High performance liquid chromatography --- Chromatography, High pressure liquid. --- Handbooks, manuals, etc. --- Analysis. --- Amino Acids --- Chromatography, High Pressure Liquid --- isolation & purification. --- methods. --- High performance liquid chromatography. --- Amino acids - Separation - Handbooks, manuals, etc --- Peptides - Separation - Handbooks, manuals, etc --- Proteins - Separation - Handbooks, manuals, etc --- High performance liquid chromatography - Handbooks, manuals, etc --- HPLC --- AMINO-ACIDES --- PEPTIDES --- PROTEINES --- BUTOPROZINE --- H.P.L.C.
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