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Biological techniques --- General biochemistry --- Molecular biology --- Nucleic acid probes --- Histochemistry --- GENE EXPRESSION --- NUCLEIC ACID HYBRIDIZATION --- Nucleic Acid Probes --- Nucleic Acid Hybridization. --- Nucleic Acid Probes. --- -Histochemistry --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Nucleic acid hybridization --- Acid Probes, Nucleic --- Probes, Nucleic Acid --- Genomic Hybridization --- Acid Hybridization, Nucleic --- Acid Hybridizations, Nucleic --- Genomic Hybridizations --- Hybridization, Genomic --- Hybridization, Nucleic Acid --- Hybridizations, Genomic --- Hybridizations, Nucleic Acid --- Nucleic Acid Hybridizations --- Oligonucleotide Probes --- Expression, Gene --- Expressions, Gene --- Gene Expressions --- Nucleic Acid Hybridization --- Gene expression --- In situ hybridization --- Gene Expression --- Histocytochemistry --- Biochemistry --- Histology --- DNA --- Cytochemistry --- Gene Expression Profiling --- Genes --- Genetic regulation --- Research&delete& --- Methodology --- Expression --- Research --- Gene expression - Research - Methodology --- NUCLEIC ACIDS --- PROBES --- HYBRIDIZATION --- IN SITU HYBRIDIZATION HISTOCHEMISTRY --- METHODS

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Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Me

Nucleic acid probes --- Polymerase chain reaction --- Virus diseases --- -Molecular diagnosis --- 57.083 --- 616-093 --- HIV human immunodeficiency virus --- NASBA --- PCR polymerase chain reaction --- biochemie --- biologie --- blotting --- chemiluminescentie --- diagnostiek --- geneeskunde --- hybridisatie --- kwaliteitscontrole --- laboratoriumbiologie --- ligase kettingreactie --- nucleïnezuren --- virusinfecties --- virussen --- Viral diseases --- Viral infections --- Virus infections --- Communicable diseases --- Medical virology --- Pathogenic viruses --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Molecular diagnosis --- Polymerase chain reaction. --- Nucleic acid probes. --- Molecular diagnosis. --- Virus diseases - - Molecular diagnosis

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Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Nucleic acid probes --- Nucleic acids --- DNA --- DNA Probes --- Molecular Probe Techniques --- Nucleic Acid Amplification Techniques --- Analysis --- analysis --- Nucleic acid probes. --- Nucleic acids -- Analysis. --- Nucleic Acid Probes --- Investigative Techniques --- Nucleic Acids --- Genetic Techniques --- Molecular Probes --- Nucleic Acids, Nucleotides, and Nucleosides --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Laboratory Chemicals --- Chemicals and Drugs --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Analysis. --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Biochemistry. --- Human genetics. --- Cytology. --- Botany. --- Biochemistry, general. --- Human Genetics. --- Cell Biology. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Floristic botany --- Nucleic acids - Analysis --- DNA - analysis

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Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on

Analytical biochemistry --- Neoplastic Processes --- Pathologic Processes --- Neoplasms --- Pathological Conditions, Signs and Symptoms --- Diseases --- Neoplasm Metastasis --- Metastases, Neoplasm --- Metastasis, Neoplasm --- Neoplasm Metastases --- Metastase --- Metastasis --- Metastases --- Symptoms and General Pathology --- Benign Neoplasms --- Malignancy --- Malignant Neoplasms --- Neoplasia --- Neoplasm --- Neoplasms, Benign --- Cancer --- Tumors --- Benign Neoplasm --- Cancers --- Malignancies --- Malignant Neoplasm --- Neoplasias --- Neoplasm, Benign --- Neoplasm, Malignant --- Neoplasms, Malignant --- Tumor --- Medical Oncology --- Pathological Processes --- Processes, Pathologic --- Processes, Pathological --- Processes, Neoplastic --- secondary --- Inositol phosphates. --- Inositol phosphates - Laboratory manuals. --- Phospholipids - Laboratory manuals. --- Phospholipids. --- Glycerophospholipids --- Phosphotransferases (Alcohol Group Acceptor) --- Investigative Techniques --- Phosphotransferases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phosphatidic Acids --- Transferases --- Glycerophosphates --- Enzymes --- Phospholipids --- Enzymes and Coenzymes --- Membrane Lipids --- Chemicals and Drugs --- Lipids --- Phosphatidylinositol 3-Kinases --- 1-Phosphatidylinositol 4-Kinase --- Phosphatidylinositols --- Clinical Laboratory Techniques --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Clinical Laboratory Diagnoses --- Clinical Laboratory Testings --- Clinical Laboratory Tests --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Clinical Laboratory Test --- Clinical Laboratory Testing --- Diagnose, Clinical Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Laboratory Test, Clinical --- Laboratory Testing, Clinical --- Technique, Clinical Laboratory --- Test, Clinical Laboratory --- Testing, Clinical Laboratory --- Laboratories --- Medical Laboratory Science --- Clinical Laboratory Services --- Inositide Phospholipids --- Inositol Phospholipids --- Phosphatidyl Inositol --- Phosphatidylinositol --- Inositide Phospholipid --- Inositol Phosphoglyceride --- Inositol Phosphoglycerides --- Inositol Phospholipid --- Phosphoinositide --- Phosphoinositides --- PtdIns --- Inositol, Phosphatidyl --- Phosphoglyceride, Inositol --- Phosphoglycerides, Inositol --- Phospholipid, Inositide --- Phospholipid, Inositol --- Phospholipids, Inositide --- Phospholipids, Inositol --- PI 4-Kinase --- Phosphatidylinositiol Kinase --- Phosphatidylinositol Kinase Type II --- Phosphoinositide Kinase --- Phosphatidylinositol 4-Kinase --- PtdIns 4-Kinase --- 1 Phosphatidylinositol 4 Kinase --- Kinase, Phosphatidylinositiol --- Kinase, Phosphoinositide --- PI 4 Kinase --- Phosphatidylinositol 4 Kinase --- PtdIns 4 Kinase --- PI 3-Kinase --- PI-3K --- PI3 Kinases --- PI3-Kinase --- Phosphoinositide 3 Kinases --- Phosphoinositide 3-Hydroxykinase --- PtdIns 3-Kinases --- PI-3 Kinase --- Phosphatidylinositol-3-OH Kinase --- PtdIns 3-Kinase --- 3-Hydroxykinase, Phosphoinositide --- Kinase, PI-3 --- Kinase, Phosphatidylinositol-3-OH --- Kinases, PI3 --- Kinases, Phosphoinositide 3 --- PI 3 Kinase --- PI3 Kinase --- Phosphatidylinositol 3 Kinases --- Phosphatidylinositol 3 OH Kinase --- Phosphoinositide 3 Hydroxykinase --- PtdIns 3 Kinase --- PtdIns 3 Kinases --- Lipid --- Cell Membrane Lipid --- Cell Membrane Lipids --- Membrane Lipid --- Lipid, Cell Membrane --- Lipid, Membrane --- Lipids, Cell Membrane --- Lipids, Membrane --- Membrane Lipid, Cell --- Membrane Lipids, Cell --- Cell Membrane --- Membranes --- Coenzymes and Enzymes --- Phosphatides --- Phospholipid --- Biocatalysts --- Biocatalyst --- Enzyme --- Molecular Mechanisms of Pharmacological Action --- Calcium Glycerophosphate --- Glycerolphosphates --- Glycerolphosphate --- Glycerophosphate --- Glycerophosphate, Calcium --- Transferase --- Ammonium Phosphatidate --- Diacylglycerophosphates --- Phosphatidic Acid --- Acid, Phosphatidic --- Acids, Phosphatidic --- Phosphatidate, Ammonium --- Kinase --- Kinases --- Phosphotransferases, ATP --- Transphosphorylases --- ATP Phosphotransferases --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Glycerophospholipid --- Phosphoglyceride --- Phosphoglycerides --- 616-006.6 --- 616-006.6 Malignant epithelial tumours. Carcinomata. Cancer --- Malignant epithelial tumours. Carcinomata. Cancer --- Cancer metastasis --- Dissemination of cancer --- Metastatic cancer --- Neoplasm metastasis --- Spread of cancer --- Tumor dissemination --- Tumor metastasis --- Tumor spread --- Pathology --- Cancer invasiveness --- Cancer of unknown primary origin --- Research --- Methodology --- Dissemination --- 57.08 --- 57.08 Biological techniques. Experimental methods. Equipment --- Biological techniques. Experimental methods. Equipment --- Isoelectric focusing. --- Hydrogen-ion concentration. --- Hydrogen-ion activity --- pH (Chemistry) --- Acidity function --- Electrofocusing --- Focusing, Isoelectric --- Analytical chemistry --- Chemistry, Organic --- Electrophoresis --- Qualitative --- Nucleic acid hybridization. --- Nucleic acid probes. --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Cytogenetics --- Cytology --- Hybridization --- Technique --- RNA-protein interactions --- Interactions, RNA-protein --- Protein-RNA interactions --- RNA-protein binding --- Protein binding --- RNA-protein interactions. --- Nucleic Acids --- Amino Acids, Peptides, and Proteins --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemical Phenomena --- Proteins --- RNA --- Chemistry --- Physical Sciences & Mathematics --- Biochemistry --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Gene Products, Protein --- Gene Proteins --- Protein --- Protein Gene Products --- Proteins, Gene --- Chemical Phenomenon --- Chemical Process --- Physical Chemistry Phenomena --- Physical Chemistry Process --- Physicochemical Phenomenon --- Physicochemical Process --- Chemical Concepts --- Chemical Processes --- Physical Chemistry Concepts --- Physical Chemistry Processes --- Physicochemical Concepts --- Physicochemical Phenomena --- Physicochemical Processes --- Chemical Concept --- Chemistry Process, Physical --- Chemistry Processes, Physical --- Concept, Chemical --- Concept, Physical Chemistry --- Concept, Physicochemical --- Concepts, Chemical --- Concepts, Physical Chemistry --- Concepts, Physicochemical --- Phenomena, Chemical --- Phenomena, Physical Chemistry --- Phenomena, Physicochemical --- Phenomenon, Chemical --- Phenomenon, Physicochemical --- Physical Chemistry Concept --- Physicochemical Concept --- Process, Chemical --- Process, Physical Chemistry --- Process, Physicochemical --- Processes, Chemical --- Processes, Physical Chemistry --- Processes, Physicochemical --- Nucleic Acid --- Acid, Nucleic --- Acids, Nucleic --- Diagnostic Techniques and Procedures --- Analysis. --- Disease.