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This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation. The book consists of three sections:   Part I, entitled Lipid peroxidation and small molecule adducts focusses on the biochemical events that are involved in lipid peroxidation and to the formation of small molecules like singlet molecular oxygen. This part of the book also introduces the hexanoyl-lysine adduct. Part II, entitled Pathophysiological consequences covers a range of damaging physiological consequences of lipid peroxidation, ranging from atherosclerosis to neurodegenerative disorders. Finally, Part III, entitled Applications for diagnosis and development of functional food looks into potential diagnostic uses of lipid peroxidation, the possible beneficial effects that can be achieved and new assays in food safely that have been developed.

Lipids --- Lipids. --- Peroxidation. --- Lipides --- Lipins --- Lipoids --- Medicine. --- Cancer research. --- Food --- Posttranslational modification. --- Biomedicine. --- Cancer Research. --- Posttranslational Modification. --- Food Science. --- Biotechnology. --- Biomolecules --- Steroids --- Oncology. --- Post-translational modification . --- Food science. --- Science --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Tumors --- Synthesis --- Food—Biotechnology. --- Cancer research

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This book offers a broad overview of the concepts and research findings in immunometabolism. The immune system is made up of numerous different cell types, pathways, and components that must be able to respond rapidly to a pathogen or cancer, but must also remain quiescent in the absence of challenges. Immune cells rely on metabolic pathways to adapt to changing environments and stimuli. Additionally, these cells can be modified in function or fate by fluctuations in available nutrients. The chapters in this book describe ways in which immune cells utilize and are regulated by metabolic pathways. Topics include how immune-cell metabolism shapes immune homeostasis, and how dysregulation of these pathways can lead to immune disorders. In different contexts, such as a tumor microenvironment, immune-cell function and identity may be modified not only by cytokines and checkpoint molecules, but also by nutrient availability and other metabolic stimuli. Transcriptional reprogramming confers many of the changes in immune cell metabolism that are seen when a T-cell, for example, undergoes activation or functional adaptation to different environments. Lastly, immune cells can destructively or protectively participate in human metabolic homeostasis or disorders. This book summarizes immune-metabolism from a variety of different perspectives, including the ways in which metabolic cues, pathways, and requirements of immune cells change in conditions of homeostasis and activation. The exploration of the significance of metabolic checkpoints and other cues, particularly in the context of cancer and immune disorders, may form the foundation for the development of therapeutics.

Immune system. --- Cancer. --- Medicine. --- Cancer research. --- Immunology. --- Medical microbiology. --- Posttranslational modification. --- Biomedicine. --- Posttranslational Modification. --- Medical Microbiology. --- Cancer Research. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Immunobiology --- Life sciences --- Serology --- Cancer research --- Clinical sciences --- Medical profession --- Human biology --- Medical sciences --- Pathology --- Physicians --- Synthesis --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Immunological system --- Anatomy --- Immunology --- Post-translational modification . --- Microbiology. --- Oncology. --- Microbial biology --- Biology --- Microorganisms

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Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.

Biochemistry. --- Post-translational modification . --- Protein Science. --- Posttranslational Modification. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Synthesis --- Composition

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The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology™ series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Biochemistry. --- Post-translational modification . --- Protein Science. --- Posttranslational Modification. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Synthesis --- Composition

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The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

Post-translational modification . --- Biochemistry. --- Posttranslational Modification. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Composition --- Synthesis