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In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.

Immunology. --- Immunobiology --- Life sciences --- Serology --- Small interfering RNA --- Immune system --- Immune system - Physiology - Laboratory manuals

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Biological techniques --- Biomembranes --- Bioenergetics --- Biological transport --- CELL MEMBRANE, physiology, laboratory manuals. --- ENERGY METABOLISM, laboratory manuals. --- Membranes (Biology) --- Laboratory manuals. --- Cell membrane, physiology, laboratory manuals. --- Energy metabolism, laboratory manuals. --- Membranes (biology) --- Biological Transport. --- Cell Membrane --- Energy Metabolism. --- 577.35 --- 577.23 --- 577.23 Energy bases of life. Molecular bioenergetics --- Energy bases of life. Molecular bioenergetics --- 577.35 Biophysics of living systems --- Biophysics of living systems --- Energy Expenditure --- Bioenergetic --- Energy Expenditures --- Energy Metabolisms --- Expenditure, Energy --- Expenditures, Energy --- Metabolism, Energy --- Metabolisms, Energy --- Biologic Transport --- Transport, Biological --- Transport, Biologic --- Transport Vesicles --- Membrane Transport Proteins --- physiology. --- Biological Transport --- Energy Metabolism --- physiology

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Biochemistry --- Plants --- Botanical chemistry --- Plant physiology --- Physiologie végétale --- laboratory manuals --- physiology --- Laboratory manuals --- Manuels de laboratoire --- Basic Sciences. Molecular Biology --- Laboratory manuals. --- Plant Molecular Biology --- Plant Molecular Biology. --- Physiologie végétale --- laboratory manuals. --- Botanical chemistry - Laboratory manuals --- Plant physiology - Laboratory manuals

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptorsContains chapters

Cell receptors -- Research -- Methodology. --- Cellular signal transduction -- Research -- Methodology. --- G proteins -- Receptors. --- Signal transduction -- Physiology -- Laboratory manuals. --- Metabolism --- Biological Transport --- Receptors, Cell Surface --- Biochemical Processes --- Membrane Proteins --- Biochemical Phenomena --- Chemical Processes --- Metabolic Phenomena --- Phenomena and Processes --- Chemical Phenomena --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Receptors, G-Protein-Coupled --- Protein Transport --- Protein Binding --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- G proteins --- Receptors. --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Enzymology.

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Drs. Peng Liang and Arthur B. Pardee have in Differential Display Methods and Protocols assembled for the first time a comprehensive review of the state of the art of their powerful new methodology and its practical applications. The book's pioneering contributors describe all the major elements of this novel technology, including both RAP-PCR and DD using fluorescence detection, as well as their powerful strategy for identifying and cloning family-specific genes. Also provided are numerous examples-along with detailed experimental procedures-in which differentially expressed genes have been successfully identified in diverse biological systems ranging from plants to songbirds to humans. Comprehensive and on the cutting-edge, Differential Display Methods and Protocols provides readers with precise new tools for studying how gene expression is exactly regulated throughout the development of a living organism, and how the failure of this intricate control mechanism leads to pathological complications. This novel and productive methodology, fully detailed here, is already playing a major role in the development of selective antagonists and inhibitors for treating cancer, cardiovascular disease, CNS disorders, and inflammation and tissue repair. Now scientists everywhere can successfully apply this powerful set of techniques using the step-by-step procedures of its innovators.

DNA fingerprinting. --- Gene expression --- Messenger RNA --- Polymerase chain reaction. --- Research --- Methodology. --- Laboratory manuals. --- 57.088.5 --- 577.212.3 --- 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Methods and techniques for studying structures and properties of biological molecules --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Polymerase chain reaction --- DNA fingerprinting --- Cloning, Molecular --- Gene Expression --- Molecular Biology --- Polymerase Chain Reaction --- Gene Expression Regulation. --- Methodology --- methods --- physiology --- methods. --- Cytology. --- Messenger RNA - Laboratory manuals --- Gene expression - Research - Methodology --- Cloning, Molecular - methods - laboratory manuals --- Gene Expression - physiology - laboratory manuals --- Molecular Biology - methods - laboratory manuals --- Polymerase Chain Reaction - methods - Laboratory Manuals --- Molecular Biology - methods. --- Cloning, Molecular - methods. --- Polymerase Chain Reaction - methods.