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This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Periodonci --- Microorganismes patògens --- Patògens --- Microorganismes --- Bacteris patògens --- Fongs patògens --- Microorganismes fitopatògens --- Protozous patògens --- Microbiologia mèdica --- Virulència (Microbiologia) --- Membrana periodòntica --- Pericement --- Perident --- Periosti alveolar --- Teixit periapical --- Teixits (Histologia) --- Fixació epitelial --- Lligament periodontal --- Microbiology. --- Dentistry. --- Medicine—Research. --- Biology—Research. --- Biomedical Research. --- Dental surgery --- Odontology --- Surgery, Dental --- Medicine --- Oral medicine --- Teeth --- Microbial biology --- Biology --- Microorganisms

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In the pursuit of technological advancement in the field of biotechnology and pharmaceutical industries to counteract health issues, bacterial infections remain a major cause of morbidity and mortality. The ability of bacterial pathogens to form biofilms further agglomerates the situation by showing resistance to conventional antibiotics. To overcome this serious issue, bioactive metabolites and other natural products were exploited to combat bacterial infections and biofilm-related health consequences. Natural products exhibited promising results in vitro, however; their efficacy in in vivo conditions remain obscured due to their low-solubility, bioavailability, and biocompatibility issues. In this scenario, nanotechnological interventions provide a multifaceted platform for targeted delivery of bioactive compounds by slow and sustained release of drug-like compounds. The unique physico-chemical properties, biocompatibility and eco-friendly nature of bioinspired nanostructures has revolutionized the field of biology to eradicate microbial infections and biofilm-related complications. The green-nanotechnology based metal and metal oxide nanoparticles and polymeric nanoparticles have been regularly employed for antimicrobial and antibiofilm applications without causing damage to host tissues. The implications of these nanoparticles toward achieving sustainability in agriculture by providing systemic resistance against a variety of phytopathogens therefore plays crucial role in growth and crop productivity. Also the advent of smart and hybrid nanomaterials such as metal-based polymer nanocomposites, lipid-based nanomaterials and liposomes have the inherent potential to eradicate bacterial biofilm-related infections in an efficient manner. The recent development of carbon-based nanomaterials such as carbon nanotubes (CNTs) and silica based nanomaterials such as mesoporous silica nanoparticles (MSNs) also exploit a target of dreadful healthcare conditions such as cancer, immunomodulatory diseases, and microbial infections, as well as biofilm-related issues owing to their stability profile, biocompatibility, and unique physio-chemical properties. Recently novel physical approaches such as photothermal therapy (PTT) and antimicrobial photodynamic therapy (aPDT) also revolutionized conventional strategies and are engaged in eradicating microbial biofilm-related infections and related health consequences. These promising advancements in the development of novel strategies to treat microbial infections and biofilm-related multidrug resistance (MDR) phenomenon may provide new avenues and aid to conventional antimicrobial therapeutics.

Microbiology. --- Nanotechnology. --- Plant breeding. --- Plant genetics. --- Microbial genetics. --- Microbial genomics. --- Plant systematics. --- Plant taxonomy. --- Plant Breeding/Biotechnology. --- Plant Genetics and Genomics. --- Microbial Genetics and Genomics. --- Plant Systematics/Taxonomy/Biogeography. --- Botanical classification --- Botanical systematics --- Botanical taxonomy --- Botany --- Classification --- Plant biosystematics --- Plant classification --- Plant systematics --- Plant taxonomy --- Systematic botany --- Systematics (Botany) --- Taxonomy, Plant --- Plant taxonomists --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Plants --- Crops --- Agriculture --- Breeding --- Molecular technology --- Nanoscale technology --- High technology --- Microbial biology --- Biology --- Nanotechnology --- Research. --- Microbiological research --- Microbiologia agrícola --- Microorganismes patògens --- Nanotecnologia --- Enginyeria molecular --- Tecnologia molecular --- Alta tecnologia --- Electrònica molecular --- Espintrònica --- Materials nanoestructurats --- Nanoelectrònica --- Nanolitografia --- Nanomedicina --- Nanoquímica --- Patògens --- Microorganismes --- Bacteris patògens --- Fongs patògens --- Microorganismes fitopatògens --- Protozous patògens --- Microbiologia mèdica --- Virulència (Microbiologia) --- Microbiologia --- Bacteriologia agrícola --- Plant biotechnology. --- Plant Biotechnology. --- Plant Genetics. --- Microbial Genetics. --- Plant Evolution. --- Evolution. --- Plant evolution --- Evolution (Biology) --- Crop biotechnology --- Agricultural biotechnology --- Phylogeny --- Biotechnology

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Actinobacteria --- Microbiologia mèdica --- Microbiologia clínica --- Ciències de la salut --- Microbiologia --- Bacteriologia mèdica --- Diagnòstic microbiològic --- Infeccions --- Malalties infeccioses --- Micologia mèdica --- Microbiologia oral --- Microbiologia sanitària --- Microbiologia veterinària --- Virologia mèdica --- Microorganismes patògens --- Actinomycetes --- Gram-positive bacteria --- Actinobacteria.

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Mycobacteria --- Mycobactériacées --- Periodicals --- Périodiques --- Mycobacterium Infections. --- Mycobacteria. --- Life Sciences --- Micro and Molecular Biology --- epidemiology of mycobacterium --- molecular biology of mycobacterium --- clinical features of mycobacterium --- TB drug resistance --- infection control --- preventional strategies --- Mycobacteriaceae --- Infections, Mycobacterium --- Infection, Mycobacterium --- Mycobacterium Infection --- Actinomycetales --- General microbiology --- tb drug resistance --- Micobacteris. --- Microbiologia mèdica. --- Bacteriologia mèdica. --- Microbiologia mèdica --- Bacteris patògens --- Malalties bacterianes --- Microbiologia clínica --- Ciències de la salut --- Microbiologia --- Bacteriologia mèdica --- Diagnòstic microbiològic --- Infeccions --- Malalties infeccioses --- Micologia mèdica --- Microbiologia oral --- Microbiologia sanitària --- Microbiologia veterinària --- Virologia mèdica --- Microorganismes patògens --- Micobacterium --- Mycobacterium tuberculosis

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One of the most exciting developments in the field of bacterial pathogenesis in recent years is the discovery that many pathogens utilize complex nanomachines to deliver bacterially encoded effector proteins into eukaryotic and prokaryotic target cells to modulate a variety of cellular functions for the pathogen’s benefit. These protein-delivery machines include the type III secretion system (T3SS), which is widespread in nature and encoded not only by bacteria pathogenic to vertebrates or plants, but also by bacteria that are symbiotic to plants or insects. Because they are essential virulence factors for many important human pathogens, these systems are emerging as a prime target for the development of new-generation, anti-infective drugs. This book reviews our current understanding of these intriguing injection machines as well as of the closely related T3SS that serves in flagella assembly. Individual chapters focus on regulation, assembly, structure, and function of the type III secretion machine and on the evolution of the secreted effector proteins. Given its scope, this book will appeal to a broad readership, including researchers and teachers in the fields of infectious diseases, host pathogen interactions, plant and animal pathogenesis, and symbiosis.

Medical microbiology. --- Infectious diseases. --- Bacteriology. --- Medical Microbiology. --- Infectious Diseases. --- Microbiology --- Pathogenic bacteria. --- Bacteriologia --- Microbiologia mèdica --- Microbiologia clínica --- Ciències de la salut --- Microbiologia --- Bacteriologia mèdica --- Diagnòstic microbiològic --- Infeccions --- Malalties infeccioses --- Micologia mèdica --- Microbiologia oral --- Microbiologia sanitària --- Microbiologia veterinària --- Virologia mèdica --- Microorganismes patògens --- Gèrmens --- Microbis --- Medicina --- Patologia --- Antisèptics --- Depuració d'aigües residuals --- Genètica bacteriana --- Productes biològics --- Bacteria --- Physiology.