Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume, with chapters written by experts in the field of cancerous tumors, details the key factors associated with liquid biopsies in solid tumors: blood-based diagnostics; circulating tumor cells; enumeration and molecular analysis (association with breast cancer); epithelialmesenchymal transition; detection and monitoring; circulating-free tumor DNA; CTCs and ctDNA; and the exosome. The field of blood-based diagnostics is rapidly evolving demonstrating the possibility of real-time molecular analysis of cancer cells and their phenotype and genotype. Circulating Tumor Cell (CTCs) have demonstrated prognostic and predictive value in advanced cancer and represents a source of tumor cells for transcriptome and genomic analysis. Most recently, the detection of genomic abnormalities in the peripheral blood by sensitive and selective PCR methods (liquid biopsy) opened to the option of a comprehensive blood-based tumor analysis. Similar information can be obtained by analysis of exosome, a natural packaging and messaging system being explored in advanced malignancies. The final frontier is the evaluation of immune cells determinant of innate and adaptive immunity.

Oncology. Neoplasms --- PCR (polymerase chain reaction) --- tumoren --- genotype --- fenotypen --- oncologie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.

Human biochemistry --- PCR (polymerase chain reaction) --- medische biochemie --- biochemie --- moleculaire biologie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Human biochemistry --- PCR (polymerase chain reaction) --- medische biochemie --- biochemie --- moleculaire biologie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This book deals with a potentially sight threatening complication - rupture of the posterior capsule – during cataract surgery. This handbook covers the management of this problem from 3 perspectives: 1.Identifying patients at higher risk and measures to manage such patients by surgical discipline 2.Intraoperative management of posterior capsular rupture (PCR) to minimize long-term complications 3.Post-operative care for a patient who has a PCR Cataract surgery is the most commonly performed surgical procedure in ophthalmology and despite tremendous technical and technological advancements, PCR still occurs. PCR occurs both in the hands of experienced senior surgeons and the neophyte surgeons, although with a higher frequency in the latter group. Additionally, certain types of cataracts are prone to this development. If managed properly in a timely manner the eventual outcome may be no different from that of an uncomplicated case. However, improper management may lead on to serious complications with a higher incidence of permanent visual disability. Written for experienced and not-so-experienced eye surgeons alike to understand and manage PCR. .

Ophthalmology --- Surgery --- Human medicine --- PCR (polymerase chain reaction) --- geneeskunde --- chirurgie --- oftalmologie --- cataract

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

*Molecular Forensics* offers a comprehensive coverage of the increasingly important role that molecular analysis plays within forensic science. Starting with a broad introduction of modern forensic molecular technologies, the text covers key issues from the initial scenes of crime sampling to the use of evidential material in the prosecution of legal cases. The book also explores the questions raised by the growing debate on the applications of national DNA databases and the resulting challenges of developing, maintaining and curating such vast data structures. The broader range of applications to non-human cases is also discussed, as are the statistical pitfalls of using so-called unique data such as DNA profiles, and the ethical considerations of national DNA databases.

An invaluable reference for students taking courses within the Forensic and Biomedical sciences, and also useful for practitioners in the field looking for a broad overview of the subject.

- Provides a comprehensive overview of modern forensic molecular technologies.

- Explores the growing debate on the applications of national DNA databases.

- Discusses the initial phases of investigation to the conclusion of cases involving molecular forensic analysis.

Legal medicine --- Molecular biology --- PCR (polymerase chain reaction) --- DNA (deoxyribonucleic acid) --- biometrie --- gerechtelijke geneeskunde --- moleculaire biologie