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This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.

Coronavirus --- Coronaviridae --- Orthocoronavirinae --- Nidovirales --- Betacoronavirus --- Virology. --- Microbiology

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This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.

Virology. --- Veterinary microbiology. --- Veterinary Microbiology. --- Agricultural microbiology --- Medical microbiology --- Communicable diseases in animals --- Microbiology --- Coronavirus --- Microbiologia veterinària --- Microbiologia animal --- Microbiologia mèdica --- Bacteriologia veterinària --- Micologia veterinària --- Coronaviridae --- Orthocoronavirinae --- Nidovirales --- Betacoronavirus

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Where did this frightening disease come from? How did it spread? And will the world be any better prepared if it returns? For the first time, these and other questions are answered in this remarkable inside account of what really took place in those fateful months of 2003 when severe acute respiratory syndrome threatened to engulf the world.

Communicable diseases -- Prevention. --- Communicable Diseases, Emerging. --- Communicable diseases. --- Epidemics -- Prevention. --- Public health -- United States. --- SARS (Disease) -- Epidemiology. --- SARS (Disease) -- Prevention -- Government policy -- United States. --- SARS (Disease) -- Research -- United States. --- Severe acute respiratory syndrome-related coronavirus. --- Severe Acute Respiratory Syndrome --- Disease Outbreaks --- Communicable Diseases, Emerging --- Coronavirus Infections --- Coronavirus --- Public Health --- Respiratory Tract Infections --- Communicable Diseases --- Coronaviridae Infections --- Coronaviridae --- Respiratory Tract Diseases --- Environment and Public Health --- Infections. --- Disease. --- Nidovirales Infections --- Nidovirales --- Delivery of Health Care. --- Bacterial Infections and Mycoses --- RNA Viruses --- RNA Virus Infections --- Vertebrate Viruses --- Viruses --- Virus Diseases --- Organisms

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SARS was the ?rst new plague of the twenty-?rst century. Within months, it spread worldwide from its “birthplace” in Guangdong Province, China, affecting over 8,000 people in 25 countries and territories across ?ve continents. SARS exposed the vulnerability of our modern globalised world to the spread of a new emerging infection. SARS (or a similar new emerging disease) could neither have spread so rapidly nor had such a great global impact even 50 years ago, and arguably, it was itself a product of our global inter-connectedness. Increasing af?uence and a demand for wild-game as exotic food led to the development of large trade of live animal and game animal markets where many species of wild and domestic animals were co-housed, providing the ideal opportunities for inter-species tra- mission of viruses and other microbes. Once such a virus jumped species and attacked humans, the increased human mobility allowed the virus the opportunity for rapid spread. An infected patient from Guangdong who stayed for one day at a hotel in Hong Kong led to the transmission of the disease to 16 other guests who travelled on to seed outbreaks of the disease in Toronto, Singapore, and Vietnam, as well as within Hong Kong itself. The virus exploited the practices used in modern intensive care of patients with severe respiratory disease and the weakness in infection control practices within our health care systems to cause outbreaks within hospitals, further amplifying the spread of the disease. Health-care itself has become a two-edged sword.

Molecular Biology -- methods. --- SARS (Disease) -- Molecular aspects. --- SARS Virus. --- Genetics --- Biochemistry --- Coronavirus --- Molecular Biology --- SARS Virus --- Biological Science Disciplines --- Coronaviridae --- Chemistry --- Biology --- Nidovirales --- Natural Science Disciplines --- Disciplines and Occupations --- RNA Viruses --- Viruses --- Vertebrate Viruses --- Organisms --- Medicine --- Respiratory System Diseases --- Health & Biological Sciences --- Coronavirus infections. --- SARS (Disease) --- Epidemiology. --- Acute respiratory syndrome, Severe --- Respiratory syndrome, Severe acute --- Severe acute respiratory syndrome --- Coronavirus diseases --- Life sciences. --- Medical microbiology. --- Virology. --- Infectious diseases. --- Cell biology. --- Microbiology. --- Microbial genetics. --- Microbial genomics. --- Life Sciences. --- Microbial Genetics and Genomics. --- Medical Microbiology. --- Cell Biology. --- Infectious Diseases. --- Coronavirus infections --- Respiratory infections --- Syndromes --- Virus diseases --- Cytology. --- Medical virology. --- Emerging infectious diseases. --- Medical microbiology --- Virology --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Emerging infections --- New infectious diseases --- Re-emerging infectious diseases --- Reemerging infectious diseases --- Communicable diseases --- Microbial biology --- Microorganisms --- Genomics --- Microbial genetics --- Microbiology --- methods --- SARS Coronavirus --- SARS-Related Coronavirus --- Severe acute respiratory syndrome-related coronavirus --- Urbani SARS-Associated Coronavirus --- SARS-Associated Coronavirus --- SARS-CoV --- Severe Acute Respiratory Syndrome Virus --- Coronavirus, SARS --- Coronavirus, SARS-Associated --- Coronavirus, SARS-Related --- Coronavirus, Urbani SARS-Associated --- SARS Associated Coronavirus --- SARS Related Coronavirus --- SARS-Associated Coronavirus, Urbani --- Severe acute respiratory syndrome related coronavirus --- Urbani SARS Associated Coronavirus