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This text gives a broad, but concise, coverage of gene cloning and manipulation, suitable for undergraduates and beginning graduate students. Assuming only general biochemical knowledge, it stresses the concepts underlying particular types of cloning vector, and uses examples to illustrate them, rather than simply presenting a mass of detailed lists and vector maps. The book starts by describing the principles behind cloning DNA in E. coli, the enzymes used, the range of cloning vectors available, and how to screen libraries to find particular clones. The author shows how PCR can be used as an alternative, or complementary, approach. He then goes on to describe how sequences can be exploited, after cloning and identification, by site-directed mutagenesis and over-expression. The book finishes with a detailed presentation of the genetic manipulation of other organisms, including other bacteria, yeast, plants, insects, and mammals.

genetische engineering (manipulatie) --- kloneren (klonen) --- genetica (genen) --- génie génétique (ingénierie génétique) --- clonage --- génétique (gènes) --- PCR polymerase chain reaction --- bacteriofagen --- blotting --- clonen --- gastheerorganismen --- gelelektroforese --- laboratoriumbiologie --- plasmiden --- rDNA recombinant DNA --- restrictie enzymen --- vectoren biologie --- biologische technieken, experimentele methoden en uitrusting in het algemeen --- Molecular cloning --- 57.08 --- 577.21 --- biochemie --- biologie --- genetica --- genetische manipulatie --- modificatie --- moleculaire biologie --- mutagenen --- #ABIB:aimm --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.

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de prentenset voor de methode wereldoriëntatie wouw.Deze methode wereldoriëntatie is geschikt voor het eerste leerjaar.

Lager onderwijs --- Basisonderwijs --- 1e leerjaar lager onderwijs --- Wereldoriëntatie --- Biologie --- 6e leerjaar secundair onderwijs --- Voortplanting --- Menskunde --- Erfelijkheidsleer --- Evolutie --- Biotechnologie --- Voortplanting (biologie) --- #BSCH: vak: biologie --- #BSCH: vak: wetenschappen --- 371.67 --- 373.5 --- 372.857 --- biologie --- leerboeken --- secundair onderwijs --- 485.43 --- schoolboeken --- 570.12 --- Chromosomen --- Genetica --- Leermiddelen --- Moleculaire genetica --- Mutaties --- 6e leerjaar s.o --- 6e leerjaar t.s.o --- Handboek/cursus --- biotechnologie --- celdifferentiatie --- evolutietheorie --- genetica --- handboek --- mutagenen --- mutatie --- stamceltherapie --- voortplanting --- 373.5 Middelbaar onderwijs --- 373.5 Middelbare scholen. Voortgezet onderwijs --- Middelbaar onderwijs --- Middelbare scholen. Voortgezet onderwijs --- 371.67 Leerboeken. Wetenschappelijk instrumentarium. Cartografische hulpmiddelen --- Leerboeken. Wetenschappelijk instrumentarium. Cartografische hulpmiddelen --- 372.857 Biologie --(niet-universitair onderwijs) --- Biologie --(niet-universitair onderwijs) --- didactiek secundair onderwijs - biologie --- Schoolbooks - Didactic material --- Contains audio-visual material

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toxicologie --- Human genetics --- Toxicology --- Pharmacology. Therapy --- Oncology. Neoplasms --- pharmacology --- farmacologie --- biologie --- toxicology --- mutaties --- Hygiene. Public health. Protection --- Environmental protection. Environmental technology --- aangeboren afwijkingen --- antropogenetica --- Ecologie --- Erfelijkheidsleer --- Hérédité --- 575.224 --- 614.7 --- 504.75 --- Genetic toxicology --- Health risk assessment --- #KVIV --- #ECO:01.13:economie sociaal duurzaam milieu ethiek --- $?$88/12 --- 575.17 --- 575.224.46.044 --- 612.6.05 --- 575 --- 502.5:575.2 --- ecologie --- genetica --- genotoxiciteit --- milieubeheer --- 504 --- 600.2 --- 605.99 --- 614.2 --- 614.62 --- DNA --- chromosomen --- geneeskunde --- geneesmiddelen --- genetisch risico --- gezondheidszorg --- leefmilieu --- metalen --- milieu --- mutagenen --- oncologie --- roken --- Gezondheidszorg --- Milieuhygiëne --- Reproduktietoxicologie --- 504.054 --- 575.1 --- 614.3 --- Milieuvervuiling : erfelijkheid --- carcinogenen --- koolwaterstoffen --- metaalintoxicaties --- milieuhygiëne --- pesticiden --- tabak --- Assessment, Health risk --- Health hazard appraisal --- Health hazard assessment --- Health risk appraisal --- HRA (Public health) --- Human risk assessment --- Medicine, Preventive --- Public health --- Risk assessment --- Environmental health --- Genotoxicity --- Genotoxicology --- Biochemical genetics --- Medical genetics --- Carcinogenesis --- Chemical mutagenesis --- Mutations. Mutagenesis. Mutagens. --- Pollutie van lucht, water, grond--(openbare gezondheidszorg) --- Human ecology and human environment --- erfelijkheid --- milieu - leefmilieu - milieubeleid (zie ook 712) - duurzame ontwikkeling --- Milieuwetenschappen --- Andere speciale ziekten - Genetologie - Aangeboren en erfelijke afwijkingen - Chronische ziekten --- Maatschappelijke gezondheidszorg voor afzonderlijke groepen --- Milieuverontreiniging --- (zie ook: beroepskanker) --- Genetic aspects --- Genetic toxicology. --- Health risk assessment. --- Gevaarlijke stoffen --- Risico-analyse --- Gevaarlijke stoffen. --- Risico-analyse. --- 575.224 Mutations. Mutagenesis. Mutagens. --- 504.75 Human ecology and human environment --- Mutations. Mutagenesis. Mutagens --- Genetica --- Génétique --- Verontreiniging. Toxicologie. --- Verontreiniging. Genetische aspecten. --- Pollution. Toxicologie. --- Pollution. Aspects génétiques.