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Die Industrielle Mikrobiologie gewinnt sowohl in der Lebensmittel- und Pharmaindustrie als auch in der chemischen Industrie zunehmend an Bedeutung. Sie vereint das Fachwissen von Naturwissenschaftlern und Ingenieuren über die Nutzung von Bakterien und Pilzen. Diese Mikroorganismen sind in der Lage, effizient nachwachsende Rohstoffe zu verwerten und in nachhaltige Produkte umzuwandeln. So konnten in der letzten Zeit mit Hilfe der Gentechnik mikrobielle Produktionsstämme gezielt entwickelt werden, die bestimmte Metabolite oder Pharmaproteine in großen Mengen produzieren. Als innovative Querschnittsdisziplin bietet die Industrielle Mikrobiologie also wichtige Voraussetzungen für die Entwicklung konkurrenzfähiger Produkte auf der Basis umweltschonender Verfahren. In dieser Branche besteht außerdem ein zunehmender Bedarf an gut ausgebildeten Fachkräften. Dieses neue Lehrbuch wurde von erfahrenen Wissenschaftlern aus Hochschulen und der Industrie verfasst. Es soll Studierende aus Life Science-Bachelorstudiengängen sowie fortgeschrittene Studierende der Chemie oder der Ingenieurwissenschaften in die Industrielle Mikrobiologie einführen. Es vermittelt die Grundlagen der Entwicklung von Produktionsstämmen und erklärt spezielle Verfahren zur Herstellung mikrobieller Produkte. Dabei wird aufgezeigt, wie das Potential der Mikroorganismen optimal genutzt werden kann. Zunächst wird ein Überblick über die geschichtliche Entwicklung der Industrielle Mikrobiologie und eine Einführung in die Bioverfahrenstechnik gegeben. Anschließend werden in 10 Kapiteln ausgewählte mikrobielle Verfahren zur Herstellung von Lebensmitteln, organischen Säuren, Alkoholen, Aminosäuren, Vitaminen, Antibiotika, Pharmaproteinen, Enzymen, Biopolymeren sowie Steroiden und Aromastoffen beschrieben. Im letzten Kapitel wird am Beispiel der biologischen Abwasserreinigung aufgezeigt, dass die Mikroorganismen nicht nur ein enormes Synthese-, sondern auch ein großes Abbaupotential besitzen, mit dem sie einen Beitrag zu den Stoffwechselkreisläufen auf unserer Erde leisten. Die Autoren wünschen sich, dass dieses Lehrbuch das Interesse vieler Studierender an diesem spannenden Lehr- und Forschungsgebiet weckt und sie daraus Nutzen ziehen können, um dann selbst zur weiteren Entwicklung der Industriellen Mikrobiologie beizutragen.  .

Microbiology. --- Biotechnology. --- Microbial genetics. --- Microbial genomics. --- Applied Microbiology. --- Microbial Genetics and Genomics.

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This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Microbial genetics. --- Microbial genomics. --- Microbiology. --- Microbial Genetics and Genomics. --- Microbial biology --- Biology --- Microorganisms --- Genomics --- Microbial genetics --- Genetics --- Microbiology

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This book provides a comprehensive and complete overview of biomarkers in clinical practice for inflammatory bowel disease (IBD) bringing together the literature in a clear and concise manner. The book bridges the gap between growing knowledge at the bench and current and future applications of biomarkers in clinical practice. The central structure of the book focuses on prognostic and predictive biomarkers in IBD with an emphasis on the fields of research and scientific techniques (genomics, proteomics and metabonomics) that have led to biomarker discovery and places these biomarkers within a clinical context to help understand their utility in clinical practice. This book will be of use to clinicians who have an interest in using biomarkers in clinical practice as well as clinician researchers and scientists involved in the biomarker research pipeline. The author team comprises experts from around the world in order to bring together the literature in an effort to inform clinicians and researchers about the current state-of-the art in biomarker discovery. It is intended to assist future research efforts and indicate how biomarkers might be best applied to clinical practice both at present and in the future.

Gastroenterology. --- Microbial genetics. --- Microbial genomics. --- Proteomics. --- Microbial Genetics and Genomics. --- Molecular biology --- Proteins --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Internal medicine --- Digestive organs --- Diseases --- Gastroenterology .

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Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube.  Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.

Human genetics. --- Microbial genetics. --- Microbial genomics. --- Human Genetics. --- Microbial Genetics and Genomics. --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Heredity, Human --- Human biology --- Physical anthropology

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This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

Human genetics. --- Microbial genetics. --- Microbial genomics. --- Human Genetics. --- Microbial Genetics and Genomics. --- Genomics --- Microbial genetics --- Microorganisms --- Genetics --- Microbiology --- Heredity, Human --- Human biology --- Physical anthropology