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Catalysis. --- Membrane proteins. --- Mitochondrial membranes. --- Multienzyme complexes.

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Antigen Presentation --- Antigens --- ATP-Binding Cassette Transporters --- Proteins --- Multienzyme Complexes --- metabolism --- metabolism --- metabolism --- metabolism

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The targeted addition of ubiquitin to proteins is now known to regulate many cellular events, with the enzymes involved in protein ubiquitination linked to a variety of clinical problems, such as cancer, heart disease, and immune dysfunction. In Ubiquitin-Proteasome Protocols, hands-on leaders in the field, many of whom originally developed the methods they describe, detail cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome- dependent functions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.

Electronic books. -- local. --- Proteolytic enzymes -- Laboratory manuals. --- Ubiquitin -- Laboratory manuals. --- Multienzyme Complexes --- Ubiquitin --- Cysteine Endopeptidases --- Multiprotein Complexes --- Enzymes --- Cysteine Proteases --- Ubiquitins --- Endopeptidases --- Peptide Hydrolases --- Macromolecular Substances --- Proteins --- Enzymes and Coenzymes --- Hydrolases --- Chemicals and Drugs --- Amino Acids, Peptides, and Proteins --- Proteolytic enzymes --- Cysteine Endopeptidases. --- Multienzyme Complexes. --- Ubiquitine --- Enzymes protéolytiques --- metabolism. --- Laboratory manuals --- Manuels de laboratoire --- EPUB-LIV-FT SPRINGER-B LIVBIOLO --- Peptide hydrolases --- Proteases --- High mobility protein 20 --- HMP-20 --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Laboratory manuals.

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Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been

Anti-Bacterial agents -- Biosynthesis. --- Biological products -- Biosynthesis. --- Carbohydrates -- Biosynthesis. --- Peptide Biosynthesis --- Biological Products --- Anti-Bacterial Agents --- Polyketide Synthases --- Enzymes --- Carbohydrates --- Complex Mixtures --- Enzymes and Coenzymes --- Anti-Infective Agents --- Biochemical Processes --- Multienzyme Complexes --- Metabolism --- Chemicals and Drugs --- Carbon-Carbon Ligases --- Biochemical Phenomena --- Ligases --- Therapeutic Uses --- Metabolic Phenomena --- Chemical Processes --- Phenomena and Processes --- Chemical Phenomena --- Pharmacologic Actions --- Chemical Actions and Uses --- Biosynthesis. --- Peptides --- Polyketides --- Synthesis. --- Microbial biotechnology. --- Carbs (Carbohydrates) --- Peptide synthesis --- Acetogenins --- Biological synthesis --- Synthesis, Biological --- Microorganisms --- Biotechnology --- Biomolecules --- Organic compounds --- Glycomics --- Ketenes --- Polymers --- Biochemical engineering --- Biochemistry --- Synthetic biology --- Biochemical templates --- Industrial microbiology --- Biotechnological microorganisms --- Synthesis

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This book will describe the nuclear encoded genes and their expressed proteins of mitochondrial oxidative phosphorylation. Most of these genes occur in eukaryotic cells, but not in bacteria or archaea. The main function of mitochondria, the synthesis of ATP, is performed at subunits of proton pumps (complexes I, III, IV and V), which are encoded on mitochondrial DNA. The nuclear encoded subunits have mostly a regulatory function. However, the specific physiological functions of the nuclear encoded subunits of complexes I, III, IV, and V are mostly unknown. New data indicates that they are essential for life of higher organisms, which is characterized by an adult life without cell division (postmeiotic stage) in most tissues, after the juvenile growth. For complex IV (cytochrome c oxidase) some of these subunits occur in tissue-specific (subunits IV, VIa, VIb, VIIa, VIII), developmental-specific (subunits IV, VIa, and VIIa) as well as species-specific isoforms. Defective genes of some subunits were shown to induce mitochondrial diseases. Mitochondrial genes and human diseases will also be covered.

DNA, Mitochrondrial. --- Eukaryotic cells. --- Mitochondrial DNA. --- Mitochondrial DNA --- Mitochondrial pathology --- Eukaryotic cells --- Cytochrome c Group --- Subcellular Fractions --- Multienzyme Complexes --- Phosphorylation --- Metabolic Phenomena --- Energy Metabolism --- Organelles --- Phenomena and Processes --- Cytoplasmic Structures --- Biochemical Processes --- Cytochromes --- Cellular Structures --- Enzymes --- Chemical Processes --- Hemeproteins --- Enzymes and Coenzymes --- Biochemical Phenomena --- Cytoplasm --- Cells --- Proteins --- Chemicals and Drugs --- Intracellular Space --- Chemical Phenomena --- Anatomy --- Amino Acids, Peptides, and Proteins --- Oxidative Phosphorylation --- Electron Transport Chain Complex Proteins --- Metabolism --- Mitochondria --- Cytochromes c --- Biology --- Medicine --- Human Anatomy & Physiology --- Health & Biological Sciences --- Pathology --- Animal Biochemistry --- Genetics --- Mitochondria. --- Mitochondrial pathology. --- Phosphorylation. --- Mitochondrial disorders --- Chondriosomes --- Diseases --- Medicine. --- Human genetics. --- Gene expression. --- Cell biology. --- Biomedicine. --- Human Genetics. --- Gene Expression. --- Cell Biology. --- Cell biology --- Cellular biology --- Cytologists --- Genes --- Genetic regulation --- Heredity, Human --- Human biology --- Physical anthropology --- Clinical sciences --- Medical profession --- Life sciences --- Medical sciences --- Physicians --- Expression --- Chemical reactions --- Pathology, Cellular --- Cell organelles --- Protoplasm --- Disorders --- Cytology.