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This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Biochemistry. --- Materials—Analysis. --- Imaging systems. --- Imaging Techniques. --- Radar --- Remote sensing --- Television --- Scanning systems --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Equipment and supplies --- Composition --- Bioluminescència --- Luminescència --- Fotoluminescència --- Radiació --- Fluorescència --- Fosforescència --- Luminescència animal --- Producció de llum d'organismes vius --- Fotobiologia

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This book highlights and investigates novel solid-state luminescent properties of crystals with stimuli-responsive behavior. Several novel molecular designs for controlling crystal structures with photo-physical properties are described, with a special focus on external stimuli-responsive properties. The major goal of the material design concept was to capitalize on the chirality of crystals with stimuli-responsive properties. To allow crystals’ chirality to be controlled and modified by means of external stimulation, the axial chirality of biaryl moiety was employed and, interestingly, produced several novel mechano- and vapo-responsive luminescent properties based on crystal-to-crystal or single-crystal-to-single-crystal phase transitions. In addition, the book details how the molecular rotation of luminophores in the solid phase can be used to achieve corresponding thermal-responsive phosphorescence. The reports presented here illustrate how the author has succeeded in controlling structural factors in a bulk environment by using molecular design with linking to photo-physical properties. The content will be of great interest to researchers in the field, and to members of chemical and material science societies. .

Chemiluminescence. --- Chemoluminescence --- Luminescence --- Organometallic chemistry . --- Optical materials. --- Electronic materials. --- Physical chemistry. --- Inorganic chemistry. --- Organometallic Chemistry. --- Optical and Electronic Materials. --- Physical Chemistry. --- Inorganic Chemistry. --- Inorganic chemistry --- Chemistry --- Inorganic compounds --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Electronic materials --- Optics --- Materials --- Chemistry, Organometallic --- Metallo-organic chemistry --- Chemistry, Organic --- Luminescència --- Fotoluminescència --- Radiació --- Bioluminescència --- Fluorescència --- Fosforescència

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This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Luminescència --- Tomografia --- Biotecnologia --- Biologia --- Enginyeria --- Antropometria --- Biosíntesi --- Biotecnologia agrícola --- Biotecnologia alimentària --- Biotecnologia farmacèutica --- Biotecnologia marina --- Biotecnologia microbiana --- Enginyeria bioquímica --- Enginyeria sanitària --- Ergonomia --- Indústries biotecnològiques --- Microbiologia industrial --- Reproducció assistida --- Bioreactors --- CAT --- CT (Tomografia computada) --- Estratigrafia radiològica --- Laminografía --- Planigrafia --- Radiografia per seccions --- TAC --- Tomodensitometria --- Tomografia axial computada --- Tomografia axial computada de raigs X --- Tomografia computeritzada --- TC (Tomografia computada) --- Zonografia --- Radiografia mèdica --- Tomografia de coherència òptica --- Tomografia sísmica --- Fotoluminescència --- Radiació --- Fluorescència --- Fosforescència --- Radiology. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Radiological physics --- Physics --- Radiation --- Bioluminescència

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This volume of the series Springer Briefs in Space Life Sciences explains the physics and biology of radiation in space, defines various forms of cosmic radiation and their dosimetry, and presents a range of exposure scenarios. It also discusses the effects of radiation on human health and describes the molecular mechanisms of heavy charged particles’ deleterious effects in the body. Lastly, it discusses countermeasures and addresses the vital question: Are we ready for launch? Written for researchers in the space life sciences and space biomedicine, and for master’s students in biology, physics, and medicine, the book will also benefit all non-experts endeavoring to understand and enter space.

Molecular biology. --- Space sciences. --- Aerospace engineering. --- Astronautics. --- Cancer research. --- Ophthalmology. --- Medical physics. --- Radiation. --- Molecular Medicine. --- Space Sciences (including Extraterrestrial Physics, Space Exploration and Astronautics). --- Aerospace Technology and Astronautics. --- Cancer Research. --- Medical and Radiation Physics. --- Physics --- Radiology --- Health physics --- Health radiation physics --- Medical radiation physics --- Radiotherapy physics --- Radiation therapy physics --- Biophysics --- Medicine --- Eye --- Cancer research --- Space sciences --- Aeronautics --- Astrodynamics --- Space flight --- Space vehicles --- Aeronautical engineering --- Astronautics --- Engineering --- Science and space --- Space research --- Cosmology --- Science --- Astronomy --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biomolecules --- Systems biology --- Diseases --- Extraterrestrial radiation. --- Space radiation --- Electromagnetic waves --- Radiation --- Radiació --- Medi ambient espacial --- Ambient espacial --- Medi ambient extraterrestre --- Medi espacial --- Temps espacial --- Espai exterior --- Camps magnètics (Física còsmica) --- Matèria interstel·lar --- Raigs còsmics --- Física --- Ones --- Òptica --- Absorció de calor --- Espectroscòpia --- Espectroscòpia nuclear --- Blindatge (Radiació) --- Cambres d'ionització --- Comptadors de Geiger-Müller --- Efecte de Doppler --- Efecte de la radiació sobre els materials --- Efecte de la radiació sobre els sòlids --- Efecte de la radiació sobre les plantes --- Efectes fisiològics de les radiacions --- Electrodinàmica quàntica --- Luminescència --- Ones gravitacionals --- Polarització (Física nuclear) --- Radi (Metall) --- Radiació ionitzant --- Radiació no-ionitzant --- Radioactivitat --- Radioestèsia --- Raigs alfa --- Raigs beta --- Raigs catòdics --- So --- Teoria del transport --- Transferència radiativa --- Col·lisions (Física nuclear) --- Llum --- Radiologia --- Teoria quàntica --- Biology --- Solar system. --- Cancer. --- Biomedical Research. --- Space Physics. --- Cancer Biology. --- Medical Physics. --- Research. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Biological research --- Biomedical research --- Health Workforce