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This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Biochemistry. --- Materials—Analysis. --- Imaging systems. --- Imaging Techniques. --- Radar --- Remote sensing --- Television --- Scanning systems --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Equipment and supplies --- Composition --- Bioluminescència --- Luminescència --- Fotoluminescència --- Radiació --- Fluorescència --- Fosforescència --- Luminescència animal --- Producció de llum d'organismes vius --- Fotobiologia

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This book covers applications of R to the general discipline of radiation dosimetry and to the specific areas of luminescence dosimetry, luminescence dating, and radiation protection dosimetry. It features more than 90 detailed worked examples of R code fully integrated into the text, with extensive annotations. The book shows how researchers can use available R packages to analyze their experimental data, and how to extract the various parameters describing mathematically the luminescence signals. In each chapter, the theory behind the subject is summarized, and references are given from the literature, so that researchers can look up the details of the theory and the relevant experiments. Several chapters are dedicated to Monte Carlo methods, which are used to simulate the luminescence processes during the irradiation, heating, and optical stimulation of solids, for a wide variety of materials. This book will be useful to those who use the tools of luminescence dosimetry, including physicists, geologists, archaeologists, and for all researchers who use radiation in their research.

Statistics . --- Mathematical physics. --- Statistics and Computing/Statistics Programs. --- Theoretical, Mathematical and Computational Physics. --- Statistics for Engineering, Physics, Computer Science, Chemistry and Earth Sciences. --- Physical mathematics --- Physics --- Statistical analysis --- Statistical data --- Statistical methods --- Statistical science --- Mathematics --- Econometrics --- Luminescence. --- Afterglow (Physics) --- Light --- Light sources --- Radiation --- Luminescència --- Fotoluminescència --- Radiació --- Fluorescència --- Fosforescència --- R (Computer program language). --- GNU-S (Computer program language) --- Domain-specific programming languages --- Bioluminescència --- R (Computer program language) --- Luminescència.

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This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.

Luminescència --- Tomografia --- Biotecnologia --- Biologia --- Enginyeria --- Antropometria --- Biosíntesi --- Biotecnologia agrícola --- Biotecnologia alimentària --- Biotecnologia farmacèutica --- Biotecnologia marina --- Biotecnologia microbiana --- Enginyeria bioquímica --- Enginyeria sanitària --- Ergonomia --- Indústries biotecnològiques --- Microbiologia industrial --- Reproducció assistida --- Bioreactors --- CAT --- CT (Tomografia computada) --- Estratigrafia radiològica --- Laminografía --- Planigrafia --- Radiografia per seccions --- TAC --- Tomodensitometria --- Tomografia axial computada --- Tomografia axial computada de raigs X --- Tomografia computeritzada --- TC (Tomografia computada) --- Zonografia --- Radiografia mèdica --- Tomografia de coherència òptica --- Tomografia sísmica --- Fotoluminescència --- Radiació --- Fluorescència --- Fosforescència --- Radiology. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Radiological physics --- Physics --- Radiation --- Bioluminescència

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This volume of the series Springer Briefs in Space Life Sciences explains the physics and biology of radiation in space, defines various forms of cosmic radiation and their dosimetry, and presents a range of exposure scenarios. It also discusses the effects of radiation on human health and describes the molecular mechanisms of heavy charged particles’ deleterious effects in the body. Lastly, it discusses countermeasures and addresses the vital question: Are we ready for launch? Written for researchers in the space life sciences and space biomedicine, and for master’s students in biology, physics, and medicine, the book will also benefit all non-experts endeavoring to understand and enter space.

Molecular biology. --- Space sciences. --- Aerospace engineering. --- Astronautics. --- Cancer research. --- Ophthalmology. --- Medical physics. --- Radiation. --- Molecular Medicine. --- Space Sciences (including Extraterrestrial Physics, Space Exploration and Astronautics). --- Aerospace Technology and Astronautics. --- Cancer Research. --- Medical and Radiation Physics. --- Physics --- Radiology --- Health physics --- Health radiation physics --- Medical radiation physics --- Radiotherapy physics --- Radiation therapy physics --- Biophysics --- Medicine --- Eye --- Cancer research --- Space sciences --- Aeronautics --- Astrodynamics --- Space flight --- Space vehicles --- Aeronautical engineering --- Astronautics --- Engineering --- Science and space --- Space research --- Cosmology --- Science --- Astronomy --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biomolecules --- Systems biology --- Diseases --- Extraterrestrial radiation. --- Space radiation --- Electromagnetic waves --- Radiation --- Radiació --- Medi ambient espacial --- Ambient espacial --- Medi ambient extraterrestre --- Medi espacial --- Temps espacial --- Espai exterior --- Camps magnètics (Física còsmica) --- Matèria interstel·lar --- Raigs còsmics --- Física --- Ones --- Òptica --- Absorció de calor --- Espectroscòpia --- Espectroscòpia nuclear --- Blindatge (Radiació) --- Cambres d'ionització --- Comptadors de Geiger-Müller --- Efecte de Doppler --- Efecte de la radiació sobre els materials --- Efecte de la radiació sobre els sòlids --- Efecte de la radiació sobre les plantes --- Efectes fisiològics de les radiacions --- Electrodinàmica quàntica --- Luminescència --- Ones gravitacionals --- Polarització (Física nuclear) --- Radi (Metall) --- Radiació ionitzant --- Radiació no-ionitzant --- Radioactivitat --- Radioestèsia --- Raigs alfa --- Raigs beta --- Raigs catòdics --- So --- Teoria del transport --- Transferència radiativa --- Col·lisions (Física nuclear) --- Llum --- Radiologia --- Teoria quàntica --- Biology --- Solar system. --- Cancer. --- Biomedical Research. --- Space Physics. --- Cancer Biology. --- Medical Physics. --- Research. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Biological research --- Biomedical research --- Health Workforce