Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Advances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses.

HIV --- Retroviridae --- Molecular Biology --- Retroviruses --- Rétrovirus --- isolation and purification --- methods --- Laboratory manuals. --- Manuels de laboratoire --- Retroviruses -- Laboratory manuals. --- RNA Viruses --- Genetics --- Biochemistry --- Lentiviruses, Primate --- Oncogenic Viruses --- Viruses --- Vertebrate Viruses --- Lentivirus --- Biological Science Disciplines --- Chemistry --- Biology --- Natural Science Disciplines --- Organisms --- Disciplines and Occupations --- Health & Biological Sciences --- Microbiology & Immunology --- retrovirus --- virologie --- moleculaire biologie --- hiv virus --- leukemie --- t-cel --- antiretroviraal middel --- moleculair biologische technieken --- Rétrovirus --- EPUB-LIV-FT LIVBIOLO SPRINGER-B --- C-type RNA viruses --- Leukemogenic viruses --- Leukoviruses --- Oncornaviruses --- Oncoviruses --- RNA tumor viruses --- Oncogenic viruses --- RNA viruses --- Microbiology. --- Microbial biology --- Microorganisms

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Natural Hosts of SIV: Implications in AIDS thoroughly reviews the possible mechanisms by which African nonhuman primate natural hosts of lentiviruses remain essentially disease-free while other hosts exhibit disease and death. The book ultimately indicates directions for further research and potential translations of this compelling phenomenon into novel approaches to treat and prevent HIV. When Asian non-human primate non-natural hosts are experimentally infected with viruses isolated from African species, disease and death normally results. Meanwhile, these African nonhuman primate

AIDS (Disease) -- Congresses. --- AIDS (Disease). --- Natural immunity --- Simian immunodeficiency virus --- HIV infections --- Monkey Diseases --- Lentiviruses, Primate --- Retroviruses, Simian --- Immunologic Deficiency Syndromes --- Biological Processes --- Sexually Transmitted Diseases, Viral --- Slow Virus Diseases --- Lentivirus Infections --- Microbiological Processes --- Lentivirus --- Microbiological Phenomena --- Retroviridae Infections --- Retroviridae --- Biological Phenomena --- Primate Diseases --- Sexually Transmitted Diseases --- Immune System Diseases --- Virus Diseases --- RNA Viruses --- Diseases --- RNA Virus Infections --- Phenomena and Processes --- Animal Diseases --- Viruses --- Vertebrate Viruses --- Organisms --- HIV Infections --- Simian Acquired Immunodeficiency Syndrome --- HIV --- Host-Pathogen Interactions --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Immunology --- AIDS (Disease) --- Prevention.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Parkinson's disease, are topical and are presented in the methods-oriented style popularized by this series.This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Pa

Gene therapy. --- Retroviridae --- Adenoviridae --- RNA Viruses --- Oncogenic Viruses --- DNA Viruses --- Vertebrate Viruses --- Viruses --- Organisms --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Genetic Therapy. --- 575.08:577.21 --- 577.15 --- DNA Therapy --- Gene Therapy, Somatic --- Genetic Therapy, Gametic --- Genetic Therapy, Somatic --- Therapy, DNA --- Therapy, Gene --- Therapy, Somatic Gene --- Gene Therapy --- Somatic Gene Therapy --- Gametic Genetic Therapies --- Gametic Genetic Therapy --- Genetic Therapies --- Genetic Therapies, Gametic --- Genetic Therapies, Somatic --- Somatic Genetic Therapies --- Somatic Genetic Therapy --- Therapies, Gametic Genetic --- Therapies, Genetic --- Therapies, Somatic Genetic --- Therapy, Gametic Genetic --- Therapy, Genetic --- Therapy, Somatic Genetic --- Gene Transfer Techniques --- Genetic Services --- Genes, Transgenic, Suicide --- Genetic engineering, genetic manipulation, recombinant DNA --- Enzymes. Catalysts of biological reactions. Enzymology --- 577.15 Enzymes. Catalysts of biological reactions. Enzymology --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Animal Viruses --- Zoophaginae --- Animal Virus --- Virus --- Virus, Animal --- Viruses, Animal --- DNA Virus --- Virus, DNA --- Viruses, DNA --- Tumor Viruses --- Oncogenic Virus --- Tumor Virus --- Virus, Oncogenic --- Virus, Tumor --- Viruses, Oncogenic --- Viruses, Tumor --- RNA Rodent Viruses --- RNA Rodent Virus --- RNA Virus --- Rodent Virus, RNA --- Rodent Viruses, RNA --- Virus, RNA --- Virus, RNA Rodent --- Viruses, RNA --- Viruses, RNA Rodent --- Adenoviruses --- Adenovirus --- Leukemogenic Viruses --- Leukoviruses --- Oncornaviruses --- Oncovirinae --- Oncoviruses --- Oncoviruses, Type C --- RNA Tumor Viruses --- Retroviruses --- Type C Oncoviruses --- C Oncovirus, Type --- C Oncoviruses, Type --- Leukemogenic Virus --- Leukovirus --- Oncornavirus --- Oncovirus --- Oncovirus, Type C --- RNA Tumor Virus --- Retrovirus --- Tumor Virus, RNA --- Tumor Viruses, RNA --- Type C Oncovirus --- Virus, Leukemogenic --- Virus, RNA Tumor --- Viruses, Leukemogenic --- Viruses, RNA Tumor --- Genetic Vectors --- Microbiology --- Carcinogens --- Ichtadenovirus --- Ichtadenoviruses --- Gene Therapy. --- Adenoviruses. --- Feline immunodeficiency virus. --- Retroviruses. --- Lentiviruses. --- Viruses. --- Genetic Therapy --- Retroviridae. --- Genetic vectors --- Microorganisms --- Mobile genetic elements --- Extrachromosomal DNA --- Lentivirinae --- Lentivirus --- C-type RNA viruses --- Leukemogenic viruses --- RNA tumor viruses --- Oncogenic viruses --- RNA viruses --- Feline T-lymphotropic lentivirus --- FIV (Virus) --- FTLV (Virus) --- Immunodeficiency virus, Feline --- Lentiviruses --- DNA viruses --- Genetic engineering --- Therapeutics --- methods. --- Genetic methods